Comparison between Rose Bengal and indirect ELISA tests for detection of the antibrucella antibodies in serum of sheep in Mosul city
The aim of the present study was to determine seroprevalence of brucellosis in non vaccinated sheep in different areas inMosul city, and make a comparison between Rose Bengal and indirect ELISA tests for the diagnosis of the Brucellosis insheep. The study included examination of 228 serum samples re...
Main Authors: | , , , |
---|---|
Format: | Article |
Language: | Arabic |
Published: |
University of Mosul, College of Veterinary Medicine
2010-01-01
|
Series: | Iraqi Journal of Veterinary Sciences |
Online Access: | http://www.vetmedmosul.org/ijvs/media/10-2-4a.pdf |
_version_ | 1828520876032655360 |
---|---|
author | S. H. Arslan Kh. J. Hussain S. A. Asmaeel S. D. Hassan |
author_facet | S. H. Arslan Kh. J. Hussain S. A. Asmaeel S. D. Hassan |
author_sort | S. H. Arslan |
collection | DOAJ |
description | The aim of the present study was to determine seroprevalence of brucellosis in non vaccinated sheep in different areas inMosul city, and make a comparison between Rose Bengal and indirect ELISA tests for the diagnosis of the Brucellosis insheep. The study included examination of 228 serum samples representing 1906 sheep distributed in ten flocks.The results ofRose Bengal test showed that total seroprevalence of brucellosis was (8.7%), being highest in Talkaf (40%), while noseroprevalence of Brucellosis was recorded in Al-Rashedia, Bazwaya and Al-Chaban areas. Using indirect ELISA test the totalseroprevalence was (23.6%) and the highest percentage (100%) was in Al-Namrood area, but lowest (5.2%) in Bazwaya area.The results showed that the compatibility between the two tests was (0.380) on Kappa value which indicated that the indirectELISA test is more sensitive when compared with Rose Bengal test in detection of antibodies of Brucellosis in serum sheep. |
first_indexed | 2024-12-11T19:38:45Z |
format | Article |
id | doaj.art-938a52c4a38140e1bac6445df4f7e37d |
institution | Directory Open Access Journal |
issn | 1607-3894 2071-1255 |
language | Arabic |
last_indexed | 2024-12-11T19:38:45Z |
publishDate | 2010-01-01 |
publisher | University of Mosul, College of Veterinary Medicine |
record_format | Article |
series | Iraqi Journal of Veterinary Sciences |
spelling | doaj.art-938a52c4a38140e1bac6445df4f7e37d2022-12-22T00:53:04ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552010-01-0124 (Arabic)28992Comparison between Rose Bengal and indirect ELISA tests for detection of the antibrucella antibodies in serum of sheep in Mosul cityS. H. ArslanKh. J. HussainS. A. AsmaeelS. D. HassanThe aim of the present study was to determine seroprevalence of brucellosis in non vaccinated sheep in different areas inMosul city, and make a comparison between Rose Bengal and indirect ELISA tests for the diagnosis of the Brucellosis insheep. The study included examination of 228 serum samples representing 1906 sheep distributed in ten flocks.The results ofRose Bengal test showed that total seroprevalence of brucellosis was (8.7%), being highest in Talkaf (40%), while noseroprevalence of Brucellosis was recorded in Al-Rashedia, Bazwaya and Al-Chaban areas. Using indirect ELISA test the totalseroprevalence was (23.6%) and the highest percentage (100%) was in Al-Namrood area, but lowest (5.2%) in Bazwaya area.The results showed that the compatibility between the two tests was (0.380) on Kappa value which indicated that the indirectELISA test is more sensitive when compared with Rose Bengal test in detection of antibodies of Brucellosis in serum sheep.http://www.vetmedmosul.org/ijvs/media/10-2-4a.pdf |
spellingShingle | S. H. Arslan Kh. J. Hussain S. A. Asmaeel S. D. Hassan Comparison between Rose Bengal and indirect ELISA tests for detection of the antibrucella antibodies in serum of sheep in Mosul city Iraqi Journal of Veterinary Sciences |
title | Comparison between Rose Bengal and indirect ELISA tests for detection of the antibrucella antibodies in serum of sheep in Mosul city |
title_full | Comparison between Rose Bengal and indirect ELISA tests for detection of the antibrucella antibodies in serum of sheep in Mosul city |
title_fullStr | Comparison between Rose Bengal and indirect ELISA tests for detection of the antibrucella antibodies in serum of sheep in Mosul city |
title_full_unstemmed | Comparison between Rose Bengal and indirect ELISA tests for detection of the antibrucella antibodies in serum of sheep in Mosul city |
title_short | Comparison between Rose Bengal and indirect ELISA tests for detection of the antibrucella antibodies in serum of sheep in Mosul city |
title_sort | comparison between rose bengal and indirect elisa tests for detection of the antibrucella antibodies in serum of sheep in mosul city |
url | http://www.vetmedmosul.org/ijvs/media/10-2-4a.pdf |
work_keys_str_mv | AT sharslan comparisonbetweenrosebengalandindirectelisatestsfordetectionoftheantibrucellaantibodiesinserumofsheepinmosulcity AT khjhussain comparisonbetweenrosebengalandindirectelisatestsfordetectionoftheantibrucellaantibodiesinserumofsheepinmosulcity AT saasmaeel comparisonbetweenrosebengalandindirectelisatestsfordetectionoftheantibrucellaantibodiesinserumofsheepinmosulcity AT sdhassan comparisonbetweenrosebengalandindirectelisatestsfordetectionoftheantibrucellaantibodiesinserumofsheepinmosulcity |