Comparison between Rose Bengal and indirect ELISA tests for detection of the antibrucella antibodies in serum of sheep in Mosul city

The aim of the present study was to determine seroprevalence of brucellosis in non vaccinated sheep in different areas inMosul city, and make a comparison between Rose Bengal and indirect ELISA tests for the diagnosis of the Brucellosis insheep. The study included examination of 228 serum samples re...

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Main Authors: S. H. Arslan, Kh. J. Hussain, S. A. Asmaeel, S. D. Hassan
Format: Article
Language:Arabic
Published: University of Mosul, College of Veterinary Medicine 2010-01-01
Series:Iraqi Journal of Veterinary Sciences
Online Access:http://www.vetmedmosul.org/ijvs/media/10-2-4a.pdf
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author S. H. Arslan
Kh. J. Hussain
S. A. Asmaeel
S. D. Hassan
author_facet S. H. Arslan
Kh. J. Hussain
S. A. Asmaeel
S. D. Hassan
author_sort S. H. Arslan
collection DOAJ
description The aim of the present study was to determine seroprevalence of brucellosis in non vaccinated sheep in different areas inMosul city, and make a comparison between Rose Bengal and indirect ELISA tests for the diagnosis of the Brucellosis insheep. The study included examination of 228 serum samples representing 1906 sheep distributed in ten flocks.The results ofRose Bengal test showed that total seroprevalence of brucellosis was (8.7%), being highest in Talkaf (40%), while noseroprevalence of Brucellosis was recorded in Al-Rashedia, Bazwaya and Al-Chaban areas. Using indirect ELISA test the totalseroprevalence was (23.6%) and the highest percentage (100%) was in Al-Namrood area, but lowest (5.2%) in Bazwaya area.The results showed that the compatibility between the two tests was (0.380) on Kappa value which indicated that the indirectELISA test is more sensitive when compared with Rose Bengal test in detection of antibodies of Brucellosis in serum sheep.
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spelling doaj.art-938a52c4a38140e1bac6445df4f7e37d2022-12-22T00:53:04ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552010-01-0124 (Arabic)28992Comparison between Rose Bengal and indirect ELISA tests for detection of the antibrucella antibodies in serum of sheep in Mosul cityS. H. ArslanKh. J. HussainS. A. AsmaeelS. D. HassanThe aim of the present study was to determine seroprevalence of brucellosis in non vaccinated sheep in different areas inMosul city, and make a comparison between Rose Bengal and indirect ELISA tests for the diagnosis of the Brucellosis insheep. The study included examination of 228 serum samples representing 1906 sheep distributed in ten flocks.The results ofRose Bengal test showed that total seroprevalence of brucellosis was (8.7%), being highest in Talkaf (40%), while noseroprevalence of Brucellosis was recorded in Al-Rashedia, Bazwaya and Al-Chaban areas. Using indirect ELISA test the totalseroprevalence was (23.6%) and the highest percentage (100%) was in Al-Namrood area, but lowest (5.2%) in Bazwaya area.The results showed that the compatibility between the two tests was (0.380) on Kappa value which indicated that the indirectELISA test is more sensitive when compared with Rose Bengal test in detection of antibodies of Brucellosis in serum sheep.http://www.vetmedmosul.org/ijvs/media/10-2-4a.pdf
spellingShingle S. H. Arslan
Kh. J. Hussain
S. A. Asmaeel
S. D. Hassan
Comparison between Rose Bengal and indirect ELISA tests for detection of the antibrucella antibodies in serum of sheep in Mosul city
Iraqi Journal of Veterinary Sciences
title Comparison between Rose Bengal and indirect ELISA tests for detection of the antibrucella antibodies in serum of sheep in Mosul city
title_full Comparison between Rose Bengal and indirect ELISA tests for detection of the antibrucella antibodies in serum of sheep in Mosul city
title_fullStr Comparison between Rose Bengal and indirect ELISA tests for detection of the antibrucella antibodies in serum of sheep in Mosul city
title_full_unstemmed Comparison between Rose Bengal and indirect ELISA tests for detection of the antibrucella antibodies in serum of sheep in Mosul city
title_short Comparison between Rose Bengal and indirect ELISA tests for detection of the antibrucella antibodies in serum of sheep in Mosul city
title_sort comparison between rose bengal and indirect elisa tests for detection of the antibrucella antibodies in serum of sheep in mosul city
url http://www.vetmedmosul.org/ijvs/media/10-2-4a.pdf
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