Terminal complement complex C5b-9 and C5b assay in sera of COVID-19 patients with different disease severities
Objective: The aim of this study is to find out the link between the involvement of complement activation in the inflammatory reactions in COVID-19 patients, the deterioration of the clinical status and development of sever COVID-19 in those patients. Methodology: The study included 274 COVID-19 pat...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Zagazig University, Faculty of Medicine
2022-10-01
|
| Series: | Microbes and Infectious Diseases |
| Subjects: | |
| Online Access: | https://mid.journals.ekb.eg/article_261883_a3edd5e4a63df2c2e4e6c70bfc4b4951.pdf |
| _version_ | 1797989256090615808 |
|---|---|
| author | Asmaa Nasr El-Din Kamal Ata |
| author_facet | Asmaa Nasr El-Din Kamal Ata |
| author_sort | Asmaa Nasr El-Din |
| collection | DOAJ |
| description | Objective: The aim of this study is to find out the link between the involvement of complement activation in the inflammatory reactions in COVID-19 patients, the deterioration of the clinical status and development of sever COVID-19 in those patients. Methodology: The study included 274 COVID-19 patients, divided into three groups; group1: severe COVID-19 patients (n=37), group 2; moderate COVID-19 severity patients (n=78), group 3; mild COVID-19 severity patients (n=159). Serum levels of C-reactive protein, D-dimer, and ferritin were measured in the three patient groups, and the patients were subjected to CT chest imaging. Serum levels of the tested biomarkers were measured by ELISA at diagnosis. Results: Sever COVID-19 patients had higher serum levels of ferritin and D-dimer in comparison to patients with moderate and mild severity COVID-19 with statistically significant difference (p < /em> value 0.01 and 0.02 respectively). There was a significant elevation in the serum levels IL-6 and TNF-α in severe COVID-19 patients (488.5±112.2 and 159.6±38.3 respectively) versus moderate (206.07±53.3 and 93.5±39.5) and mild group (200.9± 52.27 and 52.9±23) respectively, (p < /em> value < 0.001). There was also a significant elevation of C5b and C5b-9 serum levels in severe COVID-19 patients (18.6±10.3 and 73.25±7.35) compared to moderate (14.11±15.6 and 143.6±170) and mild COVID-19 groups (76± 11.32 and 3.9± 3.03) respectively. Conclusion: We conclude that the severity of inflammation presented in elevated neutrophil counts and serum levels of inflammatory cytokines IL-6 and TNF in association with sever complement activation are implicated in severity and bad prognosis of COVID-19. |
| first_indexed | 2024-04-11T08:17:04Z |
| format | Article |
| id | doaj.art-938d80382e8644cf8d1cadde2c20b4ab |
| institution | Directory Open Access Journal |
| issn | 2682-4132 2682-4140 |
| language | English |
| last_indexed | 2024-04-11T08:17:04Z |
| publishDate | 2022-10-01 |
| publisher | Zagazig University, Faculty of Medicine |
| record_format | Article |
| series | Microbes and Infectious Diseases |
| spelling | doaj.art-938d80382e8644cf8d1cadde2c20b4ab2022-12-22T04:35:06ZengZagazig University, Faculty of MedicineMicrobes and Infectious Diseases2682-41322682-41402022-10-013483084110.21608/mid.2022.160620.1379261883Terminal complement complex C5b-9 and C5b assay in sera of COVID-19 patients with different disease severitiesAsmaa Nasr El-Din0Kamal Ata1Microbiology and Immunology Department, Faculty of Medicine, Sohag University, Egypt.Chest Diseases and Tuberculosis Department, Faculty of Medicine, Sohag University, Egypt.Objective: The aim of this study is to find out the link between the involvement of complement activation in the inflammatory reactions in COVID-19 patients, the deterioration of the clinical status and development of sever COVID-19 in those patients. Methodology: The study included 274 COVID-19 patients, divided into three groups; group1: severe COVID-19 patients (n=37), group 2; moderate COVID-19 severity patients (n=78), group 3; mild COVID-19 severity patients (n=159). Serum levels of C-reactive protein, D-dimer, and ferritin were measured in the three patient groups, and the patients were subjected to CT chest imaging. Serum levels of the tested biomarkers were measured by ELISA at diagnosis. Results: Sever COVID-19 patients had higher serum levels of ferritin and D-dimer in comparison to patients with moderate and mild severity COVID-19 with statistically significant difference (p < /em> value 0.01 and 0.02 respectively). There was a significant elevation in the serum levels IL-6 and TNF-α in severe COVID-19 patients (488.5±112.2 and 159.6±38.3 respectively) versus moderate (206.07±53.3 and 93.5±39.5) and mild group (200.9± 52.27 and 52.9±23) respectively, (p < /em> value < 0.001). There was also a significant elevation of C5b and C5b-9 serum levels in severe COVID-19 patients (18.6±10.3 and 73.25±7.35) compared to moderate (14.11±15.6 and 143.6±170) and mild COVID-19 groups (76± 11.32 and 3.9± 3.03) respectively. Conclusion: We conclude that the severity of inflammation presented in elevated neutrophil counts and serum levels of inflammatory cytokines IL-6 and TNF in association with sever complement activation are implicated in severity and bad prognosis of COVID-19.https://mid.journals.ekb.eg/article_261883_a3edd5e4a63df2c2e4e6c70bfc4b4951.pdfkey words: complementcovid-19c5b. c5b-9 |
| spellingShingle | Asmaa Nasr El-Din Kamal Ata Terminal complement complex C5b-9 and C5b assay in sera of COVID-19 patients with different disease severities Microbes and Infectious Diseases key words: complement covid-19 c5b. c5b-9 |
| title | Terminal complement complex C5b-9 and C5b assay in sera of COVID-19 patients with different disease severities |
| title_full | Terminal complement complex C5b-9 and C5b assay in sera of COVID-19 patients with different disease severities |
| title_fullStr | Terminal complement complex C5b-9 and C5b assay in sera of COVID-19 patients with different disease severities |
| title_full_unstemmed | Terminal complement complex C5b-9 and C5b assay in sera of COVID-19 patients with different disease severities |
| title_short | Terminal complement complex C5b-9 and C5b assay in sera of COVID-19 patients with different disease severities |
| title_sort | terminal complement complex c5b 9 and c5b assay in sera of covid 19 patients with different disease severities |
| topic | key words: complement covid-19 c5b. c5b-9 |
| url | https://mid.journals.ekb.eg/article_261883_a3edd5e4a63df2c2e4e6c70bfc4b4951.pdf |
| work_keys_str_mv | AT asmaanasreldin terminalcomplementcomplexc5b9andc5bassayinseraofcovid19patientswithdifferentdiseaseseverities AT kamalata terminalcomplementcomplexc5b9andc5bassayinseraofcovid19patientswithdifferentdiseaseseverities |