Irisin Gene Delivery Ameliorates Burn-Induced Sensory and Motor Neuropathy
Burn-related neuropathy is common and often involves pain, paresthesia, or muscle weakness. Irisin, an exercise-induced myokine after cleavage from its membrane precursor fibronectin type III domain-containing 5 (FNDC5), exhibits neuroprotective and anti-inflammatory activities. A rat model of third...
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MDPI AG
2020-10-01
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author | Shu-Hung Huang Shih-Ming Yang Jing-Jou Lo Sheng-Hua Wu Ming-Hong Tai |
author_facet | Shu-Hung Huang Shih-Ming Yang Jing-Jou Lo Sheng-Hua Wu Ming-Hong Tai |
author_sort | Shu-Hung Huang |
collection | DOAJ |
description | Burn-related neuropathy is common and often involves pain, paresthesia, or muscle weakness. Irisin, an exercise-induced myokine after cleavage from its membrane precursor fibronectin type III domain-containing 5 (FNDC5), exhibits neuroprotective and anti-inflammatory activities. A rat model of third-degree burn on the right hind paw was used to investigate the therapeutic role of irisin/FNDC5. Rats received burn injury and were treated with intrathecal recombinant adenovirus containing the irisin sequence (Ad-irisin) at 3 weeks postburn. One week later, mechanical allodynia was examined. The expression of irisin in cerebrospinal fluid (CSF) was detected. Ipsilateral gastrocnemius muscle and lumbar spinal cord were also obtained for further investigation. Furthermore, the anti-apoptotic effect of recombinant irisin in SH-SY5Y cells was evaluated through tumor necrosis factor alpha (TNFα) stimulus to mimic burn injury. We noted intrathecal Ad-irisin attenuated pain sensitization and gastrocnemius muscle atrophy by modulating the level of irisin in CSF, and the expression of neuronal FNDC5/irisin and TNFα in the spinal cord. Ad-irisin also ameliorated neuronal apoptosis in both dorsal and ventral horns. Furthermore, recombinant irisin attenuated TNFα-induced SH-SY5Y cell apoptosis. In summary, irisin attenuated allodynia and muscle wasting by ameliorating neuroinflammation-induced neuronal apoptosis. |
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issn | 1661-6596 1422-0067 |
language | English |
last_indexed | 2024-03-10T15:26:43Z |
publishDate | 2020-10-01 |
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spelling | doaj.art-939276939146418695a8601870aa72eb2023-11-20T17:59:16ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-10-012120779810.3390/ijms21207798Irisin Gene Delivery Ameliorates Burn-Induced Sensory and Motor NeuropathyShu-Hung Huang0Shih-Ming Yang1Jing-Jou Lo2Sheng-Hua Wu3Ming-Hong Tai4Division of Plastic Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung 807, TaiwanInstitute of Biomedical Sciences, National Sun Yat-Sun University, Kaohsiung 804, TaiwanDepartment of General Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 807, TaiwanDepartment of Anesthesiology, Kaohsiung Medical University Hospital, Kaohsiung 807, TaiwanInstitute of Biomedical Sciences, National Sun Yat-Sun University, Kaohsiung 804, TaiwanBurn-related neuropathy is common and often involves pain, paresthesia, or muscle weakness. Irisin, an exercise-induced myokine after cleavage from its membrane precursor fibronectin type III domain-containing 5 (FNDC5), exhibits neuroprotective and anti-inflammatory activities. A rat model of third-degree burn on the right hind paw was used to investigate the therapeutic role of irisin/FNDC5. Rats received burn injury and were treated with intrathecal recombinant adenovirus containing the irisin sequence (Ad-irisin) at 3 weeks postburn. One week later, mechanical allodynia was examined. The expression of irisin in cerebrospinal fluid (CSF) was detected. Ipsilateral gastrocnemius muscle and lumbar spinal cord were also obtained for further investigation. Furthermore, the anti-apoptotic effect of recombinant irisin in SH-SY5Y cells was evaluated through tumor necrosis factor alpha (TNFα) stimulus to mimic burn injury. We noted intrathecal Ad-irisin attenuated pain sensitization and gastrocnemius muscle atrophy by modulating the level of irisin in CSF, and the expression of neuronal FNDC5/irisin and TNFα in the spinal cord. Ad-irisin also ameliorated neuronal apoptosis in both dorsal and ventral horns. Furthermore, recombinant irisin attenuated TNFα-induced SH-SY5Y cell apoptosis. In summary, irisin attenuated allodynia and muscle wasting by ameliorating neuroinflammation-induced neuronal apoptosis.https://www.mdpi.com/1422-0067/21/20/7798irisinapoptosisneuroinflammationburn injuryneuropathy |
spellingShingle | Shu-Hung Huang Shih-Ming Yang Jing-Jou Lo Sheng-Hua Wu Ming-Hong Tai Irisin Gene Delivery Ameliorates Burn-Induced Sensory and Motor Neuropathy International Journal of Molecular Sciences irisin apoptosis neuroinflammation burn injury neuropathy |
title | Irisin Gene Delivery Ameliorates Burn-Induced Sensory and Motor Neuropathy |
title_full | Irisin Gene Delivery Ameliorates Burn-Induced Sensory and Motor Neuropathy |
title_fullStr | Irisin Gene Delivery Ameliorates Burn-Induced Sensory and Motor Neuropathy |
title_full_unstemmed | Irisin Gene Delivery Ameliorates Burn-Induced Sensory and Motor Neuropathy |
title_short | Irisin Gene Delivery Ameliorates Burn-Induced Sensory and Motor Neuropathy |
title_sort | irisin gene delivery ameliorates burn induced sensory and motor neuropathy |
topic | irisin apoptosis neuroinflammation burn injury neuropathy |
url | https://www.mdpi.com/1422-0067/21/20/7798 |
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