Identification and Enzyme Activity Comparison of Cellulase-producing Bacillus
【Objective】The research was carried out to screen out celllulase-producing strains from 14 strains of bacteria in order to provide a material basis for the development of new feed additives.【Method】The cellulase-producing strains were screened out from 14 strains by Congo red staining. Then the colo...
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| Format: | Article |
| Language: | English |
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Guangdong Academy of Agricultural Sciences
2022-04-01
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| Series: | Guangdong nongye kexue |
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| Online Access: | http://gdnykx.cnjournals.org/gdnykx/ch/reader/view_abstract.aspx?file_no=202204014 |
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| author | Xuebin JIANG Xuehong CHENG Miaopeng MA Zhongqing DENG Jiahui LI |
| author_facet | Xuebin JIANG Xuehong CHENG Miaopeng MA Zhongqing DENG Jiahui LI |
| author_sort | Xuebin JIANG |
| collection | DOAJ |
| description | 【Objective】The research was carried out to screen out celllulase-producing strains from 14 strains of bacteria in order to provide a material basis for the development of new feed additives.【Method】The cellulase-producing strains were screened out from 14 strains by Congo red staining. Then the colony morphologies of them were observed and 16S rDNA gene sequence homology was analyzed and identified. After comparing the ability of each strain to degrade cellulose by Congo red staining, the cellulase activity of each strain was detected by DNS method.【Result】Seven cellulase-producing strains were screened, including 4 strains of Bacillus licheniformis and 3 strains of Bacillus subtilis. Based on Congo red staining, it was preliminarily identified that the cellulose degradation ability of 7 Bacillus strains was showed as: LW006 > LW 005 > LW004 > LW002 > LW007 > LW003 > LW001. Among them, strain LW006 (B. subtilis) had the strongest ability to degrade cellulose, producing a clear circle with a diameter of 22.5 mm. The cellulase activities of 7 strains of Bacillus were determined by DNS method as follows: LW006 > LW005 > LW004 > LW007 > LW003 > LW002 > LW001. Among them, strain LW006 (B. subtilis) showed the highest cellulase activity with 1.22(±0.07) U/mL (P < 0.01).【Conclusion】Strain LW006 is a relatively high cellulase-producing strain, which is expected to provide new strain resources for new feed additives. |
| first_indexed | 2024-03-13T05:08:07Z |
| format | Article |
| id | doaj.art-9394666d49714fd2932aa284beda5530 |
| institution | Directory Open Access Journal |
| issn | 1004-874X |
| language | English |
| last_indexed | 2024-03-13T05:08:07Z |
| publishDate | 2022-04-01 |
| publisher | Guangdong Academy of Agricultural Sciences |
| record_format | Article |
| series | Guangdong nongye kexue |
| spelling | doaj.art-9394666d49714fd2932aa284beda55302023-06-16T06:41:57ZengGuangdong Academy of Agricultural SciencesGuangdong nongye kexue1004-874X2022-04-0149411612210.16768/j.issn.1004-874X.2022.04.014202204014Identification and Enzyme Activity Comparison of Cellulase-producing BacillusXuebin JIANG0Xuehong CHENG1Miaopeng MA2Zhongqing DENG3Jiahui LI4School of Life Science, Guangzhou University, Guangzhou 510006, ChinaCollege of Life Sciences, South China Agricultural University/Guangdong Key Laboratory of Protein Function and Regulation in Agricultural Organisms, Guangzhou 510642, ChinaCollege of Life Sciences, South China Agricultural University/Guangdong Key Laboratory of Protein Function and Regulation in Agricultural Organisms, Guangzhou 510642, ChinaCollege of Life Sciences, South China Agricultural University/Guangdong Key Laboratory of Protein Function and Regulation in Agricultural Organisms, Guangzhou 510642, ChinaCollege of Life Sciences, South China Agricultural University/Guangdong Key Laboratory of Protein Function and Regulation in Agricultural Organisms, Guangzhou 510642, China【Objective】The research was carried out to screen out celllulase-producing strains from 14 strains of bacteria in order to provide a material basis for the development of new feed additives.【Method】The cellulase-producing strains were screened out from 14 strains by Congo red staining. Then the colony morphologies of them were observed and 16S rDNA gene sequence homology was analyzed and identified. After comparing the ability of each strain to degrade cellulose by Congo red staining, the cellulase activity of each strain was detected by DNS method.【Result】Seven cellulase-producing strains were screened, including 4 strains of Bacillus licheniformis and 3 strains of Bacillus subtilis. Based on Congo red staining, it was preliminarily identified that the cellulose degradation ability of 7 Bacillus strains was showed as: LW006 > LW 005 > LW004 > LW002 > LW007 > LW003 > LW001. Among them, strain LW006 (B. subtilis) had the strongest ability to degrade cellulose, producing a clear circle with a diameter of 22.5 mm. The cellulase activities of 7 strains of Bacillus were determined by DNS method as follows: LW006 > LW005 > LW004 > LW007 > LW003 > LW002 > LW001. Among them, strain LW006 (B. subtilis) showed the highest cellulase activity with 1.22(±0.07) U/mL (P < 0.01).【Conclusion】Strain LW006 is a relatively high cellulase-producing strain, which is expected to provide new strain resources for new feed additives.http://gdnykx.cnjournals.org/gdnykx/ch/reader/view_abstract.aspx?file_no=202204014cellulasecellulose degrading bacteriascreeningidentificationbacillus |
| spellingShingle | Xuebin JIANG Xuehong CHENG Miaopeng MA Zhongqing DENG Jiahui LI Identification and Enzyme Activity Comparison of Cellulase-producing Bacillus Guangdong nongye kexue cellulase cellulose degrading bacteria screening identification bacillus |
| title | Identification and Enzyme Activity Comparison of Cellulase-producing Bacillus |
| title_full | Identification and Enzyme Activity Comparison of Cellulase-producing Bacillus |
| title_fullStr | Identification and Enzyme Activity Comparison of Cellulase-producing Bacillus |
| title_full_unstemmed | Identification and Enzyme Activity Comparison of Cellulase-producing Bacillus |
| title_short | Identification and Enzyme Activity Comparison of Cellulase-producing Bacillus |
| title_sort | identification and enzyme activity comparison of cellulase producing bacillus |
| topic | cellulase cellulose degrading bacteria screening identification bacillus |
| url | http://gdnykx.cnjournals.org/gdnykx/ch/reader/view_abstract.aspx?file_no=202204014 |
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