Is TB Testing Associated With Increased Blood Interferon-Gamma Levels?
The Republic of Ireland reports a relatively low prevalence of Johne’s disease (JD) compared to international counterparts. Postulated reasons for this include a lower average herd size and a grass-based production system. Ireland also engages in high levels of bovine tuberculosis (bTB) testing. As...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2017-10-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fvets.2017.00176/full |
| _version_ | 1818535528452063232 |
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| author | Aideen E. Kennedy Aideen E. Kennedy Jim O’Mahony Noel Byrne John MacSharry Riona G. Sayers |
| author_facet | Aideen E. Kennedy Aideen E. Kennedy Jim O’Mahony Noel Byrne John MacSharry Riona G. Sayers |
| author_sort | Aideen E. Kennedy |
| collection | DOAJ |
| description | The Republic of Ireland reports a relatively low prevalence of Johne’s disease (JD) compared to international counterparts. Postulated reasons for this include a lower average herd size and a grass-based production system. Ireland also engages in high levels of bovine tuberculosis (bTB) testing. As interferon-gamma (IFN-γ) is believed to play a key role in protecting against JD, it is our hypothesis that administration of purified protein derivative (PPD), as part of the bTB test, is associated with a systemic increase in IFN-γ production, which may potentially limit clinical progression of the disease. We studied 265 cows (202 Friesian and 63 “Non-Friesian,” e.g., JerseyX, Norwegian Red) to assess IFN-γ levels and Mycobacterium avium subspecies paratuberculosis (MAP) antibody response before and after the bTB test. As part of the compulsory annual bTB test, avian and bovine PPD were administered at two separate cervical sites. To assess IFN-γ production, blood samples were taken before and 72 h after PPD administration. MAP antibody response was assessed before and 10 days post-PPD administration. A significant increase in MAP antibody response was identified post-bTB compared to pre-bTB response (p < 0.001). Additionally, IFN-γ production significantly increased at the post-bTB time point (p < 0.001) compared to the pre-bTB test readings. This may indicate a beneficial effect of bTB testing in controlling JD. |
| first_indexed | 2024-12-11T18:26:06Z |
| format | Article |
| id | doaj.art-939c0622708f4a7c81462a35dfdc79bc |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-12-11T18:26:06Z |
| publishDate | 2017-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-939c0622708f4a7c81462a35dfdc79bc2022-12-22T00:55:05ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692017-10-01410.3389/fvets.2017.00176293473Is TB Testing Associated With Increased Blood Interferon-Gamma Levels?Aideen E. Kennedy0Aideen E. Kennedy1Jim O’Mahony2Noel Byrne3John MacSharry4Riona G. Sayers5Animal and Bioscience Research Department, Animal and Grassland Research and Innovation Centre, Teagasc, Fermoy, IrelandDepartment of Biological Sciences, Cork Institute of Technology, Cork, IrelandDepartment of Biological Sciences, Cork Institute of Technology, Cork, IrelandAnimal and Bioscience Research Department, Animal and Grassland Research and Innovation Centre, Teagasc, Fermoy, IrelandAlimentary Pharmabiotic Centre, Biosciences Institute, University College Cork, Cork, IrelandAnimal and Bioscience Research Department, Animal and Grassland Research and Innovation Centre, Teagasc, Fermoy, IrelandThe Republic of Ireland reports a relatively low prevalence of Johne’s disease (JD) compared to international counterparts. Postulated reasons for this include a lower average herd size and a grass-based production system. Ireland also engages in high levels of bovine tuberculosis (bTB) testing. As interferon-gamma (IFN-γ) is believed to play a key role in protecting against JD, it is our hypothesis that administration of purified protein derivative (PPD), as part of the bTB test, is associated with a systemic increase in IFN-γ production, which may potentially limit clinical progression of the disease. We studied 265 cows (202 Friesian and 63 “Non-Friesian,” e.g., JerseyX, Norwegian Red) to assess IFN-γ levels and Mycobacterium avium subspecies paratuberculosis (MAP) antibody response before and after the bTB test. As part of the compulsory annual bTB test, avian and bovine PPD were administered at two separate cervical sites. To assess IFN-γ production, blood samples were taken before and 72 h after PPD administration. MAP antibody response was assessed before and 10 days post-PPD administration. A significant increase in MAP antibody response was identified post-bTB compared to pre-bTB response (p < 0.001). Additionally, IFN-γ production significantly increased at the post-bTB time point (p < 0.001) compared to the pre-bTB test readings. This may indicate a beneficial effect of bTB testing in controlling JD.http://journal.frontiersin.org/article/10.3389/fvets.2017.00176/fullJohne’s diseaseTB testinterferon-gammapurified protein derivativeELISA |
| spellingShingle | Aideen E. Kennedy Aideen E. Kennedy Jim O’Mahony Noel Byrne John MacSharry Riona G. Sayers Is TB Testing Associated With Increased Blood Interferon-Gamma Levels? Frontiers in Veterinary Science Johne’s disease TB test interferon-gamma purified protein derivative ELISA |
| title | Is TB Testing Associated With Increased Blood Interferon-Gamma Levels? |
| title_full | Is TB Testing Associated With Increased Blood Interferon-Gamma Levels? |
| title_fullStr | Is TB Testing Associated With Increased Blood Interferon-Gamma Levels? |
| title_full_unstemmed | Is TB Testing Associated With Increased Blood Interferon-Gamma Levels? |
| title_short | Is TB Testing Associated With Increased Blood Interferon-Gamma Levels? |
| title_sort | is tb testing associated with increased blood interferon gamma levels |
| topic | Johne’s disease TB test interferon-gamma purified protein derivative ELISA |
| url | http://journal.frontiersin.org/article/10.3389/fvets.2017.00176/full |
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