Ruminal Methanogenic Responses to the Thiamine Supplementation in High-Concentrate Diets
Background: Thiamine supplementation in high-concentrate diets (HC) was confirmed to attenuate ruminal subacute acidosis through promoting carbohydrate metabolism, however, whether thiamine supplementation in HC impacts methane metabolism is still unclear. Therefore, in the present study, thiamine w...
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MDPI AG
2020-05-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/6/935 |
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| author | Fuguang Xue Yue Wang Yiguang Zhao Xuemei Nan Dengke Hua Fuyu Sun Liang Yang Linshu Jiang Benhai Xiong |
| author_facet | Fuguang Xue Yue Wang Yiguang Zhao Xuemei Nan Dengke Hua Fuyu Sun Liang Yang Linshu Jiang Benhai Xiong |
| author_sort | Fuguang Xue |
| collection | DOAJ |
| description | Background: Thiamine supplementation in high-concentrate diets (HC) was confirmed to attenuate ruminal subacute acidosis through promoting carbohydrate metabolism, however, whether thiamine supplementation in HC impacts methane metabolism is still unclear. Therefore, in the present study, thiamine was supplemented in the high-concentrate diets to investigate its effects on ruminal methanogens and methanogenesis process. Methods: an in vitro fermentation experiment which included three treatments: control diet (CON, concentrate/forage = 4:6; DM basis), high-concentrate diet (HC, concentrate/forage = 6:4; DM basis) and high-concentrate diet supplemented with thiamine (HCT, concentrate/forage = 6:4, DM basis; thiamine supplementation content = 180 mg/kg DM) was conducted. Each treatment concluded with four repeats, with three bottles in each repeat. The in vitro fermentation was sustained for 48h each time and repeated three times. At the end of fermentation, fermentable parameters, ruminal bacteria and methanogens community were measured. Results: HC significantly decreased ruminal pH, thiamine and acetate content, while significantly increasing propionate content compared with CON (<i>p</i> < 0.05). Conversely, thiamine supplementation significantly increased ruminal pH, acetate while significantly decreasing propionate content compared with HC treatment (<i>p</i> < 0.05). No significant difference of ruminal methanogens abundances among three treatments was observed. Thiamine supplementation significantly decreased methane production compared with CON, while no significant change was found in HCT compared with HC. Conclusion: thiamine supplementation in the high-concentrate diet (HC) could efficiently reduce CH<sub>4</sub> emissions compared with high-forage diets while without causing ruminal metabolic disorders compared with HC treatment. This study demonstrated that supplementation of proper thiamine in concentrate diets could be an effective nutritional strategy to decrease CH<sub>4</sub> production in dairy cows. |
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| spelling | doaj.art-939c61929d6644748ea33c3fe543e91f2023-11-20T02:04:15ZengMDPI AGAnimals2076-26152020-05-0110693510.3390/ani10060935Ruminal Methanogenic Responses to the Thiamine Supplementation in High-Concentrate DietsFuguang Xue0Yue Wang1Yiguang Zhao2Xuemei Nan3Dengke Hua4Fuyu Sun5Liang Yang6Linshu Jiang7Benhai Xiong8State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaState Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaBeijing Key Laboratory for Dairy Cow Nutrition, Beijing University of Agriculture, Beijing 102206, ChinaState Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaBackground: Thiamine supplementation in high-concentrate diets (HC) was confirmed to attenuate ruminal subacute acidosis through promoting carbohydrate metabolism, however, whether thiamine supplementation in HC impacts methane metabolism is still unclear. Therefore, in the present study, thiamine was supplemented in the high-concentrate diets to investigate its effects on ruminal methanogens and methanogenesis process. Methods: an in vitro fermentation experiment which included three treatments: control diet (CON, concentrate/forage = 4:6; DM basis), high-concentrate diet (HC, concentrate/forage = 6:4; DM basis) and high-concentrate diet supplemented with thiamine (HCT, concentrate/forage = 6:4, DM basis; thiamine supplementation content = 180 mg/kg DM) was conducted. Each treatment concluded with four repeats, with three bottles in each repeat. The in vitro fermentation was sustained for 48h each time and repeated three times. At the end of fermentation, fermentable parameters, ruminal bacteria and methanogens community were measured. Results: HC significantly decreased ruminal pH, thiamine and acetate content, while significantly increasing propionate content compared with CON (<i>p</i> < 0.05). Conversely, thiamine supplementation significantly increased ruminal pH, acetate while significantly decreasing propionate content compared with HC treatment (<i>p</i> < 0.05). No significant difference of ruminal methanogens abundances among three treatments was observed. Thiamine supplementation significantly decreased methane production compared with CON, while no significant change was found in HCT compared with HC. Conclusion: thiamine supplementation in the high-concentrate diet (HC) could efficiently reduce CH<sub>4</sub> emissions compared with high-forage diets while without causing ruminal metabolic disorders compared with HC treatment. This study demonstrated that supplementation of proper thiamine in concentrate diets could be an effective nutritional strategy to decrease CH<sub>4</sub> production in dairy cows.https://www.mdpi.com/2076-2615/10/6/935high-concentrate dietmetagenomic sequencingmethaneruminal methanogenesisthiamine |
| spellingShingle | Fuguang Xue Yue Wang Yiguang Zhao Xuemei Nan Dengke Hua Fuyu Sun Liang Yang Linshu Jiang Benhai Xiong Ruminal Methanogenic Responses to the Thiamine Supplementation in High-Concentrate Diets Animals high-concentrate diet metagenomic sequencing methane ruminal methanogenesis thiamine |
| title | Ruminal Methanogenic Responses to the Thiamine Supplementation in High-Concentrate Diets |
| title_full | Ruminal Methanogenic Responses to the Thiamine Supplementation in High-Concentrate Diets |
| title_fullStr | Ruminal Methanogenic Responses to the Thiamine Supplementation in High-Concentrate Diets |
| title_full_unstemmed | Ruminal Methanogenic Responses to the Thiamine Supplementation in High-Concentrate Diets |
| title_short | Ruminal Methanogenic Responses to the Thiamine Supplementation in High-Concentrate Diets |
| title_sort | ruminal methanogenic responses to the thiamine supplementation in high concentrate diets |
| topic | high-concentrate diet metagenomic sequencing methane ruminal methanogenesis thiamine |
| url | https://www.mdpi.com/2076-2615/10/6/935 |
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