Coilin as a regulator of NF-kB mediated inflammation in preeclampsia
The nuclear factor-Kappa B (NF-κB) pathway is a crucial mediator of inflammatory signaling. Aberrant activation of NF-κB is associated with several disorders including preeclampsia (PE). Many regulators of the NF-κB pathway have been identified, including microRNAs (miRNAs). Specifically, miR-517-3p...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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The Company of Biologists
2022-07-01
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| Series: | Biology Open |
| Subjects: | |
| Online Access: | http://bio.biologists.org/content/11/7/bio059326 |
| _version_ | 1811180684876709888 |
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| author | Madelyn K. Logan Katheryn E. Lett Douglas M. McLaurin Michael D. Hebert |
| author_facet | Madelyn K. Logan Katheryn E. Lett Douglas M. McLaurin Michael D. Hebert |
| author_sort | Madelyn K. Logan |
| collection | DOAJ |
| description | The nuclear factor-Kappa B (NF-κB) pathway is a crucial mediator of inflammatory signaling. Aberrant activation of NF-κB is associated with several disorders including preeclampsia (PE). Many regulators of the NF-κB pathway have been identified, including microRNAs (miRNAs). Specifically, miR-517-3p targets mRNA encoding TNFAIP3 Interacting Protein 1 (TNIP1), an inhibitor of NF-κB signaling. Activation of NF-κB increases production of the cytokine TNF superfamily member 15 (TNFSF15), leading to the upregulation of anti-angiogenic soluble vascular endothelial growth factor receptor 1 (sFlt-1). We have previously observed that Cajal bodies (CBs), subnuclear domains, are associated with the chromosome 19 miRNA gene cluster (C19MC), which encodes miR-517-3p. We have also found that coilin, the CB marker protein, is a positive regulator of miRNA biogenesis. Here we report that coilin is a regulator of miR-517-3p, sFlt-1, TNIP1, TNFSF15 and NF-κB activation, and this regulation is influenced by hypoxia. We also report that coilin and CBs are induced in the reduced uterine perfusion pressure (RUPP) rat model of PE. Collectively, the data presented here implicate coilin as a novel regulator of NF-κB activation and sFlt-1 upregulation. |
| first_indexed | 2024-04-11T09:07:24Z |
| format | Article |
| id | doaj.art-939ef360516e49c79d3ba07615413738 |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-04-11T09:07:24Z |
| publishDate | 2022-07-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-939ef360516e49c79d3ba076154137382022-12-22T04:32:36ZengThe Company of BiologistsBiology Open2046-63902022-07-0111710.1242/bio.059326059326Coilin as a regulator of NF-kB mediated inflammation in preeclampsiaMadelyn K. Logan0Katheryn E. Lett1Douglas M. McLaurin2Michael D. Hebert3 Department of Cell and Molecular Biology, The University of Mississippi Medical Center, Jackson, MS 39216-4505, USA Department of Cell and Molecular Biology, The University of Mississippi Medical Center, Jackson, MS 39216-4505, USA Department of Cell and Molecular Biology, The University of Mississippi Medical Center, Jackson, MS 39216-4505, USA Department of Cell and Molecular Biology, The University of Mississippi Medical Center, Jackson, MS 39216-4505, USA The nuclear factor-Kappa B (NF-κB) pathway is a crucial mediator of inflammatory signaling. Aberrant activation of NF-κB is associated with several disorders including preeclampsia (PE). Many regulators of the NF-κB pathway have been identified, including microRNAs (miRNAs). Specifically, miR-517-3p targets mRNA encoding TNFAIP3 Interacting Protein 1 (TNIP1), an inhibitor of NF-κB signaling. Activation of NF-κB increases production of the cytokine TNF superfamily member 15 (TNFSF15), leading to the upregulation of anti-angiogenic soluble vascular endothelial growth factor receptor 1 (sFlt-1). We have previously observed that Cajal bodies (CBs), subnuclear domains, are associated with the chromosome 19 miRNA gene cluster (C19MC), which encodes miR-517-3p. We have also found that coilin, the CB marker protein, is a positive regulator of miRNA biogenesis. Here we report that coilin is a regulator of miR-517-3p, sFlt-1, TNIP1, TNFSF15 and NF-κB activation, and this regulation is influenced by hypoxia. We also report that coilin and CBs are induced in the reduced uterine perfusion pressure (RUPP) rat model of PE. Collectively, the data presented here implicate coilin as a novel regulator of NF-κB activation and sFlt-1 upregulation.http://bio.biologists.org/content/11/7/bio059326coilincajal bodymir-517-3psflt-1preeclampsia |
| spellingShingle | Madelyn K. Logan Katheryn E. Lett Douglas M. McLaurin Michael D. Hebert Coilin as a regulator of NF-kB mediated inflammation in preeclampsia Biology Open coilin cajal body mir-517-3p sflt-1 preeclampsia |
| title | Coilin as a regulator of NF-kB mediated inflammation in preeclampsia |
| title_full | Coilin as a regulator of NF-kB mediated inflammation in preeclampsia |
| title_fullStr | Coilin as a regulator of NF-kB mediated inflammation in preeclampsia |
| title_full_unstemmed | Coilin as a regulator of NF-kB mediated inflammation in preeclampsia |
| title_short | Coilin as a regulator of NF-kB mediated inflammation in preeclampsia |
| title_sort | coilin as a regulator of nf kb mediated inflammation in preeclampsia |
| topic | coilin cajal body mir-517-3p sflt-1 preeclampsia |
| url | http://bio.biologists.org/content/11/7/bio059326 |
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