Expression of Basement Membrane Molecules by Wharton Jelly Stem Cells (WJSC) in Full-Term Human Umbilical Cords, Cell Cultures and Microtissues
Wharton’s jelly stem cells (WJSC) from the human umbilical cord (UC) are one of the most promising mesenchymal stem cells (MSC) in tissue engineering (TE) and advanced therapies. The cell niche is a key element for both, MSC and fully differentiated tissues, to preserve their unique features. The ba...
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MDPI AG
2023-02-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/12/4/629 |
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| author | David Sánchez-Porras Daniel Durand-Herrera Ramón Carmona Cristina Blanco-Elices Ingrid Garzón Michela Pozzobon Sebastián San Martín Miguel Alaminos Óscar Darío García-García Jesús Chato-Astrain Víctor Carriel |
| author_facet | David Sánchez-Porras Daniel Durand-Herrera Ramón Carmona Cristina Blanco-Elices Ingrid Garzón Michela Pozzobon Sebastián San Martín Miguel Alaminos Óscar Darío García-García Jesús Chato-Astrain Víctor Carriel |
| author_sort | David Sánchez-Porras |
| collection | DOAJ |
| description | Wharton’s jelly stem cells (WJSC) from the human umbilical cord (UC) are one of the most promising mesenchymal stem cells (MSC) in tissue engineering (TE) and advanced therapies. The cell niche is a key element for both, MSC and fully differentiated tissues, to preserve their unique features. The basement membrane (BM) is an essential structure during embryonic development and in adult tissues. Epithelial BMs are well-known, but similar structures are present in other histological structures, such as in peripheral nerve fibers, myocytes or chondrocytes. Previous studies suggest the expression of some BM molecules within the Wharton’s Jelly (WJ) of UC, but the distribution pattern and full expression profile of these molecules have not been yet elucidated. In this sense, the aim of this histological study was to evaluate the expression of main BM molecules within the WJ, cultured WJSC and during WJSC microtissue (WJSC-MT) formation process. Results confirmed the presence of a pericellular matrix composed by the main BM molecules—collagens (IV, VII), HSPG2, agrin, laminin and nidogen—around the WJSC within UC. Additionally, ex vivo studies demonstrated the synthesis of these BM molecules, except agrin, especially during WJSC-MT formation process. The WJSC capability to synthesize main BM molecules could offer new alternatives for the generation of biomimetic-engineered substitutes where these molecules are particularly needed. |
| first_indexed | 2024-03-11T09:01:03Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-11T09:01:03Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-939fa91af7364061b5d29112fb26f3672023-11-16T19:45:01ZengMDPI AGCells2073-44092023-02-0112462910.3390/cells12040629Expression of Basement Membrane Molecules by Wharton Jelly Stem Cells (WJSC) in Full-Term Human Umbilical Cords, Cell Cultures and MicrotissuesDavid Sánchez-Porras0Daniel Durand-Herrera1Ramón Carmona2Cristina Blanco-Elices3Ingrid Garzón4Michela Pozzobon5Sebastián San Martín6Miguel Alaminos7Óscar Darío García-García8Jesús Chato-Astrain9Víctor Carriel10Tissue Engineering Group, Department of Histology, Faculty of Medicine, Universidad de Granada, 18016 Granada, SpainTissue Engineering Group, Department of Histology, Faculty of Medicine, Universidad de Granada, 18016 Granada, SpainDepartment of Cell Biology, Faculty of Sciences, Universidad de Granada, 18071 Granada, SpainTissue Engineering Group, Department of Histology, Faculty of Medicine, Universidad de Granada, 18016 Granada, SpainTissue Engineering Group, Department of Histology, Faculty of Medicine, Universidad de Granada, 18016 Granada, SpainDepartment of Women and Children’s Health, University of Padova, 35129 Padova, ItalyCentro de Investigaciones Biomédicas, Escuela de Medicina, Facultad de Medicina, Universidad de Valparaíso, Valparaíso 2520000, ChileTissue Engineering Group, Department of Histology, Faculty of Medicine, Universidad de Granada, 18016 Granada, SpainTissue Engineering Group, Department of Histology, Faculty of Medicine, Universidad de Granada, 18016 Granada, SpainTissue Engineering Group, Department of Histology, Faculty of Medicine, Universidad de Granada, 18016 Granada, SpainTissue Engineering Group, Department of Histology, Faculty of Medicine, Universidad de Granada, 18016 Granada, SpainWharton’s jelly stem cells (WJSC) from the human umbilical cord (UC) are one of the most promising mesenchymal stem cells (MSC) in tissue engineering (TE) and advanced therapies. The cell niche is a key element for both, MSC and fully differentiated tissues, to preserve their unique features. The basement membrane (BM) is an essential structure during embryonic development and in adult tissues. Epithelial BMs are well-known, but similar structures are present in other histological structures, such as in peripheral nerve fibers, myocytes or chondrocytes. Previous studies suggest the expression of some BM molecules within the Wharton’s Jelly (WJ) of UC, but the distribution pattern and full expression profile of these molecules have not been yet elucidated. In this sense, the aim of this histological study was to evaluate the expression of main BM molecules within the WJ, cultured WJSC and during WJSC microtissue (WJSC-MT) formation process. Results confirmed the presence of a pericellular matrix composed by the main BM molecules—collagens (IV, VII), HSPG2, agrin, laminin and nidogen—around the WJSC within UC. Additionally, ex vivo studies demonstrated the synthesis of these BM molecules, except agrin, especially during WJSC-MT formation process. The WJSC capability to synthesize main BM molecules could offer new alternatives for the generation of biomimetic-engineered substitutes where these molecules are particularly needed.https://www.mdpi.com/2073-4409/12/4/629umbilical cord (UC)wharton jelly stem cells (WJSC)extracellular matrix (ECM)basement membrane (BM)basal lamina (BL) stem cell-based microtissues (MT)tissue engineering (TE) |
| spellingShingle | David Sánchez-Porras Daniel Durand-Herrera Ramón Carmona Cristina Blanco-Elices Ingrid Garzón Michela Pozzobon Sebastián San Martín Miguel Alaminos Óscar Darío García-García Jesús Chato-Astrain Víctor Carriel Expression of Basement Membrane Molecules by Wharton Jelly Stem Cells (WJSC) in Full-Term Human Umbilical Cords, Cell Cultures and Microtissues Cells umbilical cord (UC) wharton jelly stem cells (WJSC) extracellular matrix (ECM) basement membrane (BM) basal lamina (BL) stem cell-based microtissues (MT) tissue engineering (TE) |
| title | Expression of Basement Membrane Molecules by Wharton Jelly Stem Cells (WJSC) in Full-Term Human Umbilical Cords, Cell Cultures and Microtissues |
| title_full | Expression of Basement Membrane Molecules by Wharton Jelly Stem Cells (WJSC) in Full-Term Human Umbilical Cords, Cell Cultures and Microtissues |
| title_fullStr | Expression of Basement Membrane Molecules by Wharton Jelly Stem Cells (WJSC) in Full-Term Human Umbilical Cords, Cell Cultures and Microtissues |
| title_full_unstemmed | Expression of Basement Membrane Molecules by Wharton Jelly Stem Cells (WJSC) in Full-Term Human Umbilical Cords, Cell Cultures and Microtissues |
| title_short | Expression of Basement Membrane Molecules by Wharton Jelly Stem Cells (WJSC) in Full-Term Human Umbilical Cords, Cell Cultures and Microtissues |
| title_sort | expression of basement membrane molecules by wharton jelly stem cells wjsc in full term human umbilical cords cell cultures and microtissues |
| topic | umbilical cord (UC) wharton jelly stem cells (WJSC) extracellular matrix (ECM) basement membrane (BM) basal lamina (BL) stem cell-based microtissues (MT) tissue engineering (TE) |
| url | https://www.mdpi.com/2073-4409/12/4/629 |
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