Y-Box Binding Protein 1 Expression in Trophoblast Cells Promotes Fetal and Placental Development
Y-box binding protein 1 (YB-1) is pivotal for the regulation of cancerogenesis and inflammation. However, its involvement in pregnancy processes such as fetal and placental development remains to be elucidated. We studied <i>Ybx1</i> (YB-1)<sup>+/−</sup> heterozygous intercro...
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MDPI AG
2020-08-01
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| Online Access: | https://www.mdpi.com/2073-4409/9/9/1942 |
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| author | Nicole Meyer Anne Schumacher Urs Coenen Katja Woidacki Hannah Schmidt Jonathan A. Lindquist Peter R. Mertens Ana C. Zenclussen |
| author_facet | Nicole Meyer Anne Schumacher Urs Coenen Katja Woidacki Hannah Schmidt Jonathan A. Lindquist Peter R. Mertens Ana C. Zenclussen |
| author_sort | Nicole Meyer |
| collection | DOAJ |
| description | Y-box binding protein 1 (YB-1) is pivotal for the regulation of cancerogenesis and inflammation. However, its involvement in pregnancy processes such as fetal and placental development remains to be elucidated. We studied <i>Ybx1</i> (YB-1)<sup>+/−</sup> heterozygous intercrossings and compared them to YB-1<sup>+/+</sup> wild-type (WT) combinations. Additionally, we generated trophoblast-specific YB-1-deficient mice by pairing FVB Cyp19-Cre females to YB-1<sup>fl/fl</sup> males. YB-1<sup>fl/fl</sup>-paired FVB WT females served as controls. Serial in vivo ultrasound measurements were performed to assess fetal and placental parameters. After sacrificing the females, implantation and abortion rates were recorded, spiral artery (SA) remodeling was analyzed and fetal and placental weights were determined. Compared to YB-1<sup>+/+</sup> counterparts, YB-1<sup>+/−</sup> females showed reduced implantation areas at gestation day (GD)10, insufficiently remodeled SAs at GD12, increased placental diameter/thickness ratios at GD14 and reduced placental and fetal weights at GD14. Compared to WT, Cyp19-Cre females with YB-1-deficient placentas showed reduced implantation areas at GD8, 10 and 12; decreased placental areas and diameters at GD10 and 12; diminished placental thicknesses at GD12; as well as reduced placental weights at GD12 and 14. In conclusion, our data suggest haploinsufficiency of YB-1 resulting in disturbed fetal and placental development. Moreover, we provide the first evidence for the relevance of trophoblast-specific YB-1 for placentation. |
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| language | English |
| last_indexed | 2024-03-10T17:00:04Z |
| publishDate | 2020-08-01 |
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| spelling | doaj.art-93a70c134f874284a15c9236782679472023-11-20T10:59:57ZengMDPI AGCells2073-44092020-08-0199194210.3390/cells9091942Y-Box Binding Protein 1 Expression in Trophoblast Cells Promotes Fetal and Placental DevelopmentNicole Meyer0Anne Schumacher1Urs Coenen2Katja Woidacki3Hannah Schmidt4Jonathan A. Lindquist5Peter R. Mertens6Ana C. Zenclussen7Experimental Obstetrics and Gynecology, Medical Faculty, Otto-von-Guericke University, 39108 Magdeburg, GermanyExperimental Obstetrics and Gynecology, Medical Faculty, Otto-von-Guericke University, 39108 Magdeburg, GermanyExperimental Obstetrics and Gynecology, Medical Faculty, Otto-von-Guericke University, 39108 Magdeburg, GermanyExperimental Obstetrics and Gynecology, Medical Faculty, Otto-von-Guericke University, 39108 Magdeburg, GermanyExperimental Obstetrics and Gynecology, Medical Faculty, Otto-von-Guericke University, 39108 Magdeburg, GermanyDepartment of Nephrology and Hypertension, Diabetes and Endocrinology, Otto-von-Guericke University, 39120 Magdeburg, GermanyDepartment of Nephrology and Hypertension, Diabetes and Endocrinology, Otto-von-Guericke University, 39120 Magdeburg, GermanyExperimental Obstetrics and Gynecology, Medical Faculty, Otto-von-Guericke University, 39108 Magdeburg, GermanyY-box binding protein 1 (YB-1) is pivotal for the regulation of cancerogenesis and inflammation. However, its involvement in pregnancy processes such as fetal and placental development remains to be elucidated. We studied <i>Ybx1</i> (YB-1)<sup>+/−</sup> heterozygous intercrossings and compared them to YB-1<sup>+/+</sup> wild-type (WT) combinations. Additionally, we generated trophoblast-specific YB-1-deficient mice by pairing FVB Cyp19-Cre females to YB-1<sup>fl/fl</sup> males. YB-1<sup>fl/fl</sup>-paired FVB WT females served as controls. Serial in vivo ultrasound measurements were performed to assess fetal and placental parameters. After sacrificing the females, implantation and abortion rates were recorded, spiral artery (SA) remodeling was analyzed and fetal and placental weights were determined. Compared to YB-1<sup>+/+</sup> counterparts, YB-1<sup>+/−</sup> females showed reduced implantation areas at gestation day (GD)10, insufficiently remodeled SAs at GD12, increased placental diameter/thickness ratios at GD14 and reduced placental and fetal weights at GD14. Compared to WT, Cyp19-Cre females with YB-1-deficient placentas showed reduced implantation areas at GD8, 10 and 12; decreased placental areas and diameters at GD10 and 12; diminished placental thicknesses at GD12; as well as reduced placental weights at GD12 and 14. In conclusion, our data suggest haploinsufficiency of YB-1 resulting in disturbed fetal and placental development. Moreover, we provide the first evidence for the relevance of trophoblast-specific YB-1 for placentation.https://www.mdpi.com/2073-4409/9/9/1942Y-box binding protein 1trophoblastfetal and placental developmentpregnancyserial high-frequency ultrasonography |
| spellingShingle | Nicole Meyer Anne Schumacher Urs Coenen Katja Woidacki Hannah Schmidt Jonathan A. Lindquist Peter R. Mertens Ana C. Zenclussen Y-Box Binding Protein 1 Expression in Trophoblast Cells Promotes Fetal and Placental Development Cells Y-box binding protein 1 trophoblast fetal and placental development pregnancy serial high-frequency ultrasonography |
| title | Y-Box Binding Protein 1 Expression in Trophoblast Cells Promotes Fetal and Placental Development |
| title_full | Y-Box Binding Protein 1 Expression in Trophoblast Cells Promotes Fetal and Placental Development |
| title_fullStr | Y-Box Binding Protein 1 Expression in Trophoblast Cells Promotes Fetal and Placental Development |
| title_full_unstemmed | Y-Box Binding Protein 1 Expression in Trophoblast Cells Promotes Fetal and Placental Development |
| title_short | Y-Box Binding Protein 1 Expression in Trophoblast Cells Promotes Fetal and Placental Development |
| title_sort | y box binding protein 1 expression in trophoblast cells promotes fetal and placental development |
| topic | Y-box binding protein 1 trophoblast fetal and placental development pregnancy serial high-frequency ultrasonography |
| url | https://www.mdpi.com/2073-4409/9/9/1942 |
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