Efficient isolation of mouse deletion mutant embryonic stem cells by CRISPR

Efficient isolation of mouse deletion mutant embryonic stem cells by CRISPR

Summary: Gene functions can be assessed in mouse embryonic stem (ES) cells and in mutant mice derived from mutant ES cells. Here, we describe an approach for efficient isolation of the ES clones carrying deletion mutations at the target genes by CRISPR-Cas9. Two sgRNAs against a target gene are co-e...

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Bibliographic Details
Main Authors: Yuhan Liu, Qian Chen, Chenglin Song, Zhen Xu, Shuting Yang, Xiajun Li
Format: Article
Language:English
Published: Elsevier 2022-06-01
Series:STAR Protocols
Subjects:
Genetics
Molecular Biology
CRISPR
Stem Cells
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722003161

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