Evolutionary paths of the cAMP-dependent protein kinase (PKA) catalytic subunits.
3',5'-cyclic adenosine monophosphate (cAMP) dependent protein kinase or protein kinase A (PKA) has served as a prototype for the large family of protein kinases that are crucially important for signal transduction in eukaryotic cells. The PKA catalytic subunits Cα and Cβ, encoded by the tw...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23593352/pdf/?tool=EBI |
| _version_ | 1818723806479384576 |
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| author | Kristoffer Søberg Tore Jahnsen Torbjørn Rognes Bjørn S Skålhegg Jon K Laerdahl |
| author_facet | Kristoffer Søberg Tore Jahnsen Torbjørn Rognes Bjørn S Skålhegg Jon K Laerdahl |
| author_sort | Kristoffer Søberg |
| collection | DOAJ |
| description | 3',5'-cyclic adenosine monophosphate (cAMP) dependent protein kinase or protein kinase A (PKA) has served as a prototype for the large family of protein kinases that are crucially important for signal transduction in eukaryotic cells. The PKA catalytic subunits Cα and Cβ, encoded by the two genes PRKACA and PRKACB, respectively, are among the best understood and characterized human kinases. Here we have studied the evolution of this gene family in chordates, arthropods, mollusks and other animals employing probabilistic methods and show that Cα and Cβ arose by duplication of an ancestral PKA catalytic subunit in a common ancestor of vertebrates. The two genes have subsequently been duplicated in teleost fishes. The evolution of the PRKACG retroposon in simians was also investigated. Although the degree of sequence conservation in the PKA Cα/Cβ kinase family is exceptionally high, a small set of signature residues defining Cα and Cβ subfamilies were identified. These conserved residues might be important for functions that are unique to the Cα or Cβ clades. This study also provides a good example of a seemingly simple phylogenetic problem which, due to a very high degree of sequence conservation and corresponding weak phylogenetic signals, combined with problematic nonphylogenetic signals, is nontrivial for state-of-the-art probabilistic phylogenetic methods. |
| first_indexed | 2024-12-17T21:16:22Z |
| format | Article |
| id | doaj.art-93ae8b053e6f4790a66e27c91ff52310 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T21:16:22Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-93ae8b053e6f4790a66e27c91ff523102022-12-21T21:32:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0184e6093510.1371/journal.pone.0060935Evolutionary paths of the cAMP-dependent protein kinase (PKA) catalytic subunits.Kristoffer SøbergTore JahnsenTorbjørn RognesBjørn S SkålheggJon K Laerdahl3',5'-cyclic adenosine monophosphate (cAMP) dependent protein kinase or protein kinase A (PKA) has served as a prototype for the large family of protein kinases that are crucially important for signal transduction in eukaryotic cells. The PKA catalytic subunits Cα and Cβ, encoded by the two genes PRKACA and PRKACB, respectively, are among the best understood and characterized human kinases. Here we have studied the evolution of this gene family in chordates, arthropods, mollusks and other animals employing probabilistic methods and show that Cα and Cβ arose by duplication of an ancestral PKA catalytic subunit in a common ancestor of vertebrates. The two genes have subsequently been duplicated in teleost fishes. The evolution of the PRKACG retroposon in simians was also investigated. Although the degree of sequence conservation in the PKA Cα/Cβ kinase family is exceptionally high, a small set of signature residues defining Cα and Cβ subfamilies were identified. These conserved residues might be important for functions that are unique to the Cα or Cβ clades. This study also provides a good example of a seemingly simple phylogenetic problem which, due to a very high degree of sequence conservation and corresponding weak phylogenetic signals, combined with problematic nonphylogenetic signals, is nontrivial for state-of-the-art probabilistic phylogenetic methods.https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23593352/pdf/?tool=EBI |
| spellingShingle | Kristoffer Søberg Tore Jahnsen Torbjørn Rognes Bjørn S Skålhegg Jon K Laerdahl Evolutionary paths of the cAMP-dependent protein kinase (PKA) catalytic subunits. PLoS ONE |
| title | Evolutionary paths of the cAMP-dependent protein kinase (PKA) catalytic subunits. |
| title_full | Evolutionary paths of the cAMP-dependent protein kinase (PKA) catalytic subunits. |
| title_fullStr | Evolutionary paths of the cAMP-dependent protein kinase (PKA) catalytic subunits. |
| title_full_unstemmed | Evolutionary paths of the cAMP-dependent protein kinase (PKA) catalytic subunits. |
| title_short | Evolutionary paths of the cAMP-dependent protein kinase (PKA) catalytic subunits. |
| title_sort | evolutionary paths of the camp dependent protein kinase pka catalytic subunits |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23593352/pdf/?tool=EBI |
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