Roux-En-Y Gastric Bypass (RYGB) Surgery during High Liquid Sucrose Diet Leads to Gut Microbiota-Related Systematic Alterations
Roux-en-Y gastric bypass (RYGB) surgery has been proven successful in weight loss and improvement of co-morbidities associated with obesity. Chronic complications such as malabsorption of micronutrients in up to 50% of patients underline the need for additional therapeutic approaches. We investigate...
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MDPI AG
2022-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/3/1126 |
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| author | Laimdota Zizmare Christina N. Boyle Sabrina Buss Sandrine Louis Laura Kuebler Ketki Mulay Ralf Krüger Lara Steinhauer Isabelle Mack Manuel Rodriguez Gomez Kristina Herfert Yvonne Ritze Christoph Trautwein |
| author_facet | Laimdota Zizmare Christina N. Boyle Sabrina Buss Sandrine Louis Laura Kuebler Ketki Mulay Ralf Krüger Lara Steinhauer Isabelle Mack Manuel Rodriguez Gomez Kristina Herfert Yvonne Ritze Christoph Trautwein |
| author_sort | Laimdota Zizmare |
| collection | DOAJ |
| description | Roux-en-Y gastric bypass (RYGB) surgery has been proven successful in weight loss and improvement of co-morbidities associated with obesity. Chronic complications such as malabsorption of micronutrients in up to 50% of patients underline the need for additional therapeutic approaches. We investigated systemic RYGB surgery effects in a liquid sucrose diet-induced rat obesity model. After consuming a diet supplemented with high liquid sucrose for eight weeks, rats underwent RYGB or control sham surgery. RYGB, sham pair-fed, and sham ad libitum-fed groups further continued on the diet after recovery. Notable alterations were revealed in microbiota composition, inflammatory markers, feces, liver, and plasma metabolites, as well as in brain neuronal activity post-surgery. Higher fecal 4-aminobutyrate (GABA) correlated with higher Bacteroidota and <i>Enterococcus</i> abundances in RYGB animals, pointing towards the altered enteric nervous system (ENS) and gut signaling. Favorable C-reactive protein (CRP), serine, glycine, and 3-hydroxybutyrate plasma profiles in RYGB rats were suggestive of reverted obesity risk. The impact of liquid sucrose diet and caloric restriction mainly manifested in fatty acid changes in the liver. Our multi-modal approach reveals complex systemic changes after RYGB surgery and points towards potential therapeutic targets in the gut-brain system to mimic the surgery mode of action. |
| first_indexed | 2024-03-09T23:50:50Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:50:50Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-93b2dffb794f489f9b9c30b5e3328c102023-11-23T16:35:21ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-01-01233112610.3390/ijms23031126Roux-En-Y Gastric Bypass (RYGB) Surgery during High Liquid Sucrose Diet Leads to Gut Microbiota-Related Systematic AlterationsLaimdota Zizmare0Christina N. Boyle1Sabrina Buss2Sandrine Louis3Laura Kuebler4Ketki Mulay5Ralf Krüger6Lara Steinhauer7Isabelle Mack8Manuel Rodriguez Gomez9Kristina Herfert10Yvonne Ritze11Christoph Trautwein12Werner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, University Hospital Tübingen, Eberhard Karls University of Tübingen, Röntgenweg 13, 72076 Tübingen, GermanyInstitute of Veterinary Physiology, University of Zurich, Winterthurerstraße 260, CH-8057 Zurich, SwitzerlandWerner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, University Hospital Tübingen, Eberhard Karls University of Tübingen, Röntgenweg 13, 72076 Tübingen, GermanyDepartment of Physiology and Biochemistry of Nutrition, Max Rubner-Institut, Haid-und-Neu-Straße 9, 76131 Karlsruhe, GermanyWerner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, University Hospital Tübingen, Eberhard Karls University of Tübingen, Röntgenweg 13, 72076 Tübingen, GermanyDepartment of Medical Psychology and Behavioral Neurobiology, Eberhard Karls University of Tübingen, Silcherstraße 5, 72076 Tübingen, GermanyDepartment of Physiology and Biochemistry of Nutrition, Max Rubner-Institut, Haid-und-Neu-Straße 9, 76131 Karlsruhe, GermanyDepartment of Physiology and Biochemistry of Nutrition, Max Rubner-Institut, Haid-und-Neu-Straße 9, 76131 Karlsruhe, GermanyDepartment of Psychosomatic Medicine and Psychotherapy, University Hospital Tübingen, Osianderstraße 5, 72076 Tübingen, GermanyDepartment of Physiology and Biochemistry of Nutrition, Max Rubner-Institut, Haid-und-Neu-Straße 9, 76131 Karlsruhe, GermanyWerner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, University Hospital Tübingen, Eberhard Karls University of Tübingen, Röntgenweg 13, 72076 Tübingen, GermanyDepartment of Medical Psychology and Behavioral Neurobiology, Eberhard Karls University of Tübingen, Silcherstraße 5, 72076 Tübingen, GermanyWerner Siemens Imaging Center, Department of Preclinical Imaging and Radiopharmacy, University Hospital Tübingen, Eberhard Karls University of Tübingen, Röntgenweg 13, 72076 Tübingen, GermanyRoux-en-Y gastric bypass (RYGB) surgery has been proven successful in weight loss and improvement of co-morbidities associated with obesity. Chronic complications such as malabsorption of micronutrients in up to 50% of patients underline the need for additional therapeutic approaches. We investigated systemic RYGB surgery effects in a liquid sucrose diet-induced rat obesity model. After consuming a diet supplemented with high liquid sucrose for eight weeks, rats underwent RYGB or control sham surgery. RYGB, sham pair-fed, and sham ad libitum-fed groups further continued on the diet after recovery. Notable alterations were revealed in microbiota composition, inflammatory markers, feces, liver, and plasma metabolites, as well as in brain neuronal activity post-surgery. Higher fecal 4-aminobutyrate (GABA) correlated with higher Bacteroidota and <i>Enterococcus</i> abundances in RYGB animals, pointing towards the altered enteric nervous system (ENS) and gut signaling. Favorable C-reactive protein (CRP), serine, glycine, and 3-hydroxybutyrate plasma profiles in RYGB rats were suggestive of reverted obesity risk. The impact of liquid sucrose diet and caloric restriction mainly manifested in fatty acid changes in the liver. Our multi-modal approach reveals complex systemic changes after RYGB surgery and points towards potential therapeutic targets in the gut-brain system to mimic the surgery mode of action.https://www.mdpi.com/1422-0067/23/3/1126RYGBmetabolomicsmicrobiomeinflammationgut-brain axisGABA |
| spellingShingle | Laimdota Zizmare Christina N. Boyle Sabrina Buss Sandrine Louis Laura Kuebler Ketki Mulay Ralf Krüger Lara Steinhauer Isabelle Mack Manuel Rodriguez Gomez Kristina Herfert Yvonne Ritze Christoph Trautwein Roux-En-Y Gastric Bypass (RYGB) Surgery during High Liquid Sucrose Diet Leads to Gut Microbiota-Related Systematic Alterations International Journal of Molecular Sciences RYGB metabolomics microbiome inflammation gut-brain axis GABA |
| title | Roux-En-Y Gastric Bypass (RYGB) Surgery during High Liquid Sucrose Diet Leads to Gut Microbiota-Related Systematic Alterations |
| title_full | Roux-En-Y Gastric Bypass (RYGB) Surgery during High Liquid Sucrose Diet Leads to Gut Microbiota-Related Systematic Alterations |
| title_fullStr | Roux-En-Y Gastric Bypass (RYGB) Surgery during High Liquid Sucrose Diet Leads to Gut Microbiota-Related Systematic Alterations |
| title_full_unstemmed | Roux-En-Y Gastric Bypass (RYGB) Surgery during High Liquid Sucrose Diet Leads to Gut Microbiota-Related Systematic Alterations |
| title_short | Roux-En-Y Gastric Bypass (RYGB) Surgery during High Liquid Sucrose Diet Leads to Gut Microbiota-Related Systematic Alterations |
| title_sort | roux en y gastric bypass rygb surgery during high liquid sucrose diet leads to gut microbiota related systematic alterations |
| topic | RYGB metabolomics microbiome inflammation gut-brain axis GABA |
| url | https://www.mdpi.com/1422-0067/23/3/1126 |
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