The lung microbiota in nontuberculous mycobacterial pulmonary disease.
<h4>Background</h4>The role of bacterial microbiota in the pathogenesis of nontuberculous mycobacterial pulmonary disease (NTM-PD) is unclear. We aimed to compare the bacterial microbiome of disease-invaded lesions and non-invaded lung tissue from NTM-PD patients.<h4>Methods</h4...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2023-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0285143 |
| _version_ | 1797805705647882240 |
|---|---|
| author | Bo-Guen Kim Noeul Kang Su-Young Kim Dae Hun Kim Hojoong Kim O Jung Kwon Hee Jae Huh Nam Yong Lee Byung Woo Jhun |
| author_facet | Bo-Guen Kim Noeul Kang Su-Young Kim Dae Hun Kim Hojoong Kim O Jung Kwon Hee Jae Huh Nam Yong Lee Byung Woo Jhun |
| author_sort | Bo-Guen Kim |
| collection | DOAJ |
| description | <h4>Background</h4>The role of bacterial microbiota in the pathogenesis of nontuberculous mycobacterial pulmonary disease (NTM-PD) is unclear. We aimed to compare the bacterial microbiome of disease-invaded lesions and non-invaded lung tissue from NTM-PD patients.<h4>Methods</h4>We analyzed lung tissues from 23 NTM-PD patients who underwent surgical lung resection. Lung tissues were collected in pairs from each patient, with one sample from a disease-involved site and the other from a non-involved site. Lung tissue microbiome libraries were constructed using 16S rRNA gene sequences (V3-V4 regions).<h4>Results</h4>Sixteen (70%) patients had Mycobacterium avium complex (MAC)-PD, and the remaining seven (30%) had Mycobacterium abscessus-PD. Compared to non-involved sites, involved sites showed greater species richness (ACE, Chao1, and Jackknife analyses, all p = 0.001); greater diversity on the Shannon index (p = 0.007); and genus-level differences (Jensen-Shannon, PERMANOVA p = 0.001). Analysis of taxonomic biomarkers using linear discriminant analysis (LDA) effect sizes (LEfSe) demonstrated that several genera, including Limnohabitans, Rahnella, Lachnospira, Flavobacterium, Megamonas, Gaiella, Subdoligranulum, Rheinheimera, Dorea, Collinsella, and Phascolarctobacterium, had significantly greater abundance in involved sites (LDA >3.00, p <0.05, and q <0.05). In contrast, Acinetobacter had significantly greater abundance at non-involved sites (LDA = 4.27, p<0.001, and q = 0.002). Several genera were differentially distributed between lung tissues from MAC-PD (n = 16) and M. abscessus-PD (n = 7), and between nodular bronchiectatic form (n = 12) and fibrocavitary form (n = 11) patients. However, there was no genus with a significant q-value.<h4>Conclusions</h4>We identified differential microbial distributions between disease-invaded and normal lung tissues from NTM-PD patients, and microbial diversity was significantly higher in disease-invaded tissues.<h4>Trial registration</h4>Clinical Trial registration number: NCT00970801. |
| first_indexed | 2024-03-13T05:56:07Z |
| format | Article |
| id | doaj.art-93b74e0f9f6f463bb1c12d5fc512d93e |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-03-13T05:56:07Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-93b74e0f9f6f463bb1c12d5fc512d93e2023-06-13T05:31:27ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01185e028514310.1371/journal.pone.0285143The lung microbiota in nontuberculous mycobacterial pulmonary disease.Bo-Guen KimNoeul KangSu-Young KimDae Hun KimHojoong KimO Jung KwonHee Jae HuhNam Yong LeeByung Woo Jhun<h4>Background</h4>The role of bacterial microbiota in the pathogenesis of nontuberculous mycobacterial pulmonary disease (NTM-PD) is unclear. We aimed to compare the bacterial microbiome of disease-invaded lesions and non-invaded lung tissue from NTM-PD patients.<h4>Methods</h4>We analyzed lung tissues from 23 NTM-PD patients who underwent surgical lung resection. Lung tissues were collected in pairs from each patient, with one sample from a disease-involved site and the other from a non-involved site. Lung tissue microbiome libraries were constructed using 16S rRNA gene sequences (V3-V4 regions).<h4>Results</h4>Sixteen (70%) patients had Mycobacterium avium complex (MAC)-PD, and the remaining seven (30%) had Mycobacterium abscessus-PD. Compared to non-involved sites, involved sites showed greater species richness (ACE, Chao1, and Jackknife analyses, all p = 0.001); greater diversity on the Shannon index (p = 0.007); and genus-level differences (Jensen-Shannon, PERMANOVA p = 0.001). Analysis of taxonomic biomarkers using linear discriminant analysis (LDA) effect sizes (LEfSe) demonstrated that several genera, including Limnohabitans, Rahnella, Lachnospira, Flavobacterium, Megamonas, Gaiella, Subdoligranulum, Rheinheimera, Dorea, Collinsella, and Phascolarctobacterium, had significantly greater abundance in involved sites (LDA >3.00, p <0.05, and q <0.05). In contrast, Acinetobacter had significantly greater abundance at non-involved sites (LDA = 4.27, p<0.001, and q = 0.002). Several genera were differentially distributed between lung tissues from MAC-PD (n = 16) and M. abscessus-PD (n = 7), and between nodular bronchiectatic form (n = 12) and fibrocavitary form (n = 11) patients. However, there was no genus with a significant q-value.<h4>Conclusions</h4>We identified differential microbial distributions between disease-invaded and normal lung tissues from NTM-PD patients, and microbial diversity was significantly higher in disease-invaded tissues.<h4>Trial registration</h4>Clinical Trial registration number: NCT00970801.https://doi.org/10.1371/journal.pone.0285143 |
| spellingShingle | Bo-Guen Kim Noeul Kang Su-Young Kim Dae Hun Kim Hojoong Kim O Jung Kwon Hee Jae Huh Nam Yong Lee Byung Woo Jhun The lung microbiota in nontuberculous mycobacterial pulmonary disease. PLoS ONE |
| title | The lung microbiota in nontuberculous mycobacterial pulmonary disease. |
| title_full | The lung microbiota in nontuberculous mycobacterial pulmonary disease. |
| title_fullStr | The lung microbiota in nontuberculous mycobacterial pulmonary disease. |
| title_full_unstemmed | The lung microbiota in nontuberculous mycobacterial pulmonary disease. |
| title_short | The lung microbiota in nontuberculous mycobacterial pulmonary disease. |
| title_sort | lung microbiota in nontuberculous mycobacterial pulmonary disease |
| url | https://doi.org/10.1371/journal.pone.0285143 |
| work_keys_str_mv | AT boguenkim thelungmicrobiotainnontuberculousmycobacterialpulmonarydisease AT noeulkang thelungmicrobiotainnontuberculousmycobacterialpulmonarydisease AT suyoungkim thelungmicrobiotainnontuberculousmycobacterialpulmonarydisease AT daehunkim thelungmicrobiotainnontuberculousmycobacterialpulmonarydisease AT hojoongkim thelungmicrobiotainnontuberculousmycobacterialpulmonarydisease AT ojungkwon thelungmicrobiotainnontuberculousmycobacterialpulmonarydisease AT heejaehuh thelungmicrobiotainnontuberculousmycobacterialpulmonarydisease AT namyonglee thelungmicrobiotainnontuberculousmycobacterialpulmonarydisease AT byungwoojhun thelungmicrobiotainnontuberculousmycobacterialpulmonarydisease AT boguenkim lungmicrobiotainnontuberculousmycobacterialpulmonarydisease AT noeulkang lungmicrobiotainnontuberculousmycobacterialpulmonarydisease AT suyoungkim lungmicrobiotainnontuberculousmycobacterialpulmonarydisease AT daehunkim lungmicrobiotainnontuberculousmycobacterialpulmonarydisease AT hojoongkim lungmicrobiotainnontuberculousmycobacterialpulmonarydisease AT ojungkwon lungmicrobiotainnontuberculousmycobacterialpulmonarydisease AT heejaehuh lungmicrobiotainnontuberculousmycobacterialpulmonarydisease AT namyonglee lungmicrobiotainnontuberculousmycobacterialpulmonarydisease AT byungwoojhun lungmicrobiotainnontuberculousmycobacterialpulmonarydisease |