Duck plague virus tegument protein vp22 plays a key role in the secondary envelopment and cell-to-cell spread
Abstract Duck plague virus (DPV) is one of the major infectious and fatal diseases of geese, ducks, and other wild waterfowl. The DPV UL49 gene product VP22 is one of the most abundant tegument proteins. However, the role of the DPV VP22 is enigmatic to be clarified. In this study, we found deletion...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-07-01
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| Series: | Veterinary Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13567-023-01191-9 |
| _version_ | 1827894978363260928 |
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| author | Liping Wu Mingshu Wang Anchun Cheng Bin Tian Juan Huang Ying Wu Qiao Yang Xumin Ou Di Sun Shaqiu Zhang Xinxin Zhao Qun Gao Yu He Dekang Zhu Shun Chen Mafeng Liu Renyong Jia |
| author_facet | Liping Wu Mingshu Wang Anchun Cheng Bin Tian Juan Huang Ying Wu Qiao Yang Xumin Ou Di Sun Shaqiu Zhang Xinxin Zhao Qun Gao Yu He Dekang Zhu Shun Chen Mafeng Liu Renyong Jia |
| author_sort | Liping Wu |
| collection | DOAJ |
| description | Abstract Duck plague virus (DPV) is one of the major infectious and fatal diseases of geese, ducks, and other wild waterfowl. The DPV UL49 gene product VP22 is one of the most abundant tegument proteins. However, the role of the DPV VP22 is enigmatic to be clarified. In this study, we found deletion of the UL49 gene resulted in reduced viral growth curve and smaller plaque size in duck embryo fibroblast (DEF) cells, confirming that DPV VP22 is required for efficient viral growth in vitro. In addition, deletion of the UL49 gene inhibited the secondary envelopment of the virus, the release of viral particles, and the spread of viruses between cells. Our study signified the importance of VP22 for DPV secondary envelopment, release, cell-to-cell spread, and accumulation of viral RNA. These findings provide a basis for further study of the function of VP22 in DPV or other herpesviruses. |
| first_indexed | 2024-03-12T22:15:06Z |
| format | Article |
| id | doaj.art-93b7909181ae4ec492807ad4f61a9f3f |
| institution | Directory Open Access Journal |
| issn | 1297-9716 |
| language | English |
| last_indexed | 2024-03-12T22:15:06Z |
| publishDate | 2023-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-93b7909181ae4ec492807ad4f61a9f3f2023-07-23T11:22:33ZengBMCVeterinary Research1297-97162023-07-0154111210.1186/s13567-023-01191-9Duck plague virus tegument protein vp22 plays a key role in the secondary envelopment and cell-to-cell spreadLiping Wu0Mingshu Wang1Anchun Cheng2Bin Tian3Juan Huang4Ying Wu5Qiao Yang6Xumin Ou7Di Sun8Shaqiu Zhang9Xinxin Zhao10Qun Gao11Yu He12Dekang Zhu13Shun Chen14Mafeng Liu15Renyong Jia16Institute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural UniversityInstitute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural UniversityInstitute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural UniversityInstitute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural UniversityInstitute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural UniversityInstitute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural UniversityInstitute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural UniversityInstitute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural UniversityInstitute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural UniversityInstitute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural UniversityInstitute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural UniversityInstitute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural UniversityInstitute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural UniversityKey Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural UniversityInstitute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural UniversityInstitute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural UniversityInstitute of Veterinary Medicine and Immunology, College of Veterinary Medicine, Sichuan Agricultural UniversityAbstract Duck plague virus (DPV) is one of the major infectious and fatal diseases of geese, ducks, and other wild waterfowl. The DPV UL49 gene product VP22 is one of the most abundant tegument proteins. However, the role of the DPV VP22 is enigmatic to be clarified. In this study, we found deletion of the UL49 gene resulted in reduced viral growth curve and smaller plaque size in duck embryo fibroblast (DEF) cells, confirming that DPV VP22 is required for efficient viral growth in vitro. In addition, deletion of the UL49 gene inhibited the secondary envelopment of the virus, the release of viral particles, and the spread of viruses between cells. Our study signified the importance of VP22 for DPV secondary envelopment, release, cell-to-cell spread, and accumulation of viral RNA. These findings provide a basis for further study of the function of VP22 in DPV or other herpesviruses.https://doi.org/10.1186/s13567-023-01191-9Duck plague virusUL49VP22envelopmentcell-to-cell spread |
| spellingShingle | Liping Wu Mingshu Wang Anchun Cheng Bin Tian Juan Huang Ying Wu Qiao Yang Xumin Ou Di Sun Shaqiu Zhang Xinxin Zhao Qun Gao Yu He Dekang Zhu Shun Chen Mafeng Liu Renyong Jia Duck plague virus tegument protein vp22 plays a key role in the secondary envelopment and cell-to-cell spread Veterinary Research Duck plague virus UL49 VP22 envelopment cell-to-cell spread |
| title | Duck plague virus tegument protein vp22 plays a key role in the secondary envelopment and cell-to-cell spread |
| title_full | Duck plague virus tegument protein vp22 plays a key role in the secondary envelopment and cell-to-cell spread |
| title_fullStr | Duck plague virus tegument protein vp22 plays a key role in the secondary envelopment and cell-to-cell spread |
| title_full_unstemmed | Duck plague virus tegument protein vp22 plays a key role in the secondary envelopment and cell-to-cell spread |
| title_short | Duck plague virus tegument protein vp22 plays a key role in the secondary envelopment and cell-to-cell spread |
| title_sort | duck plague virus tegument protein vp22 plays a key role in the secondary envelopment and cell to cell spread |
| topic | Duck plague virus UL49 VP22 envelopment cell-to-cell spread |
| url | https://doi.org/10.1186/s13567-023-01191-9 |
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