Determination of the role of DDX3 a factor involved in mammalian RNAi pathway using an shRNA-expression library.
RNA interference (RNAi) is an endogenous RNA-destruction phenomenon induced by certain double-stranded RNAs (dsRNAs). In RNAi, dsRNAs are processed into small interfering RNAs (siRNAs) which in turn trigger the cleavage of the target mRNA. Here, using a short hairpin RNA-expression library, we ident...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3602433?pdf=render |
| _version_ | 1818066930251071488 |
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| author | Vivi Kasim Shourong Wu Kazunari Taira Makoto Miyagishi |
| author_facet | Vivi Kasim Shourong Wu Kazunari Taira Makoto Miyagishi |
| author_sort | Vivi Kasim |
| collection | DOAJ |
| description | RNA interference (RNAi) is an endogenous RNA-destruction phenomenon induced by certain double-stranded RNAs (dsRNAs). In RNAi, dsRNAs are processed into small interfering RNAs (siRNAs) which in turn trigger the cleavage of the target mRNA. Here, using a short hairpin RNA-expression library, we identified a DEAD-box helicase 3, DDX3, as an essential factor involved in RNAi pathway and revealed that DDX3 is colocalized with Ago2, an essential factor in RNAi pathway that cleaves target mRNA. Results of experiments with a dominant negative mutant of DDX3 further confirmed that this factor affects the RNAi activity. Together, DDX3 functions to assure mammalian RNAi pathway. Together, our results indicate that DDX3 is a new key molecule to understand the molecular mechanism underlying RNAi pathway in mammals. |
| first_indexed | 2024-12-10T15:15:36Z |
| format | Article |
| id | doaj.art-93b925023ff941bf971982c7138ff9ec |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T15:15:36Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-93b925023ff941bf971982c7138ff9ec2022-12-22T01:43:48ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0183e5944510.1371/journal.pone.0059445Determination of the role of DDX3 a factor involved in mammalian RNAi pathway using an shRNA-expression library.Vivi KasimShourong WuKazunari TairaMakoto MiyagishiRNA interference (RNAi) is an endogenous RNA-destruction phenomenon induced by certain double-stranded RNAs (dsRNAs). In RNAi, dsRNAs are processed into small interfering RNAs (siRNAs) which in turn trigger the cleavage of the target mRNA. Here, using a short hairpin RNA-expression library, we identified a DEAD-box helicase 3, DDX3, as an essential factor involved in RNAi pathway and revealed that DDX3 is colocalized with Ago2, an essential factor in RNAi pathway that cleaves target mRNA. Results of experiments with a dominant negative mutant of DDX3 further confirmed that this factor affects the RNAi activity. Together, DDX3 functions to assure mammalian RNAi pathway. Together, our results indicate that DDX3 is a new key molecule to understand the molecular mechanism underlying RNAi pathway in mammals.http://europepmc.org/articles/PMC3602433?pdf=render |
| spellingShingle | Vivi Kasim Shourong Wu Kazunari Taira Makoto Miyagishi Determination of the role of DDX3 a factor involved in mammalian RNAi pathway using an shRNA-expression library. PLoS ONE |
| title | Determination of the role of DDX3 a factor involved in mammalian RNAi pathway using an shRNA-expression library. |
| title_full | Determination of the role of DDX3 a factor involved in mammalian RNAi pathway using an shRNA-expression library. |
| title_fullStr | Determination of the role of DDX3 a factor involved in mammalian RNAi pathway using an shRNA-expression library. |
| title_full_unstemmed | Determination of the role of DDX3 a factor involved in mammalian RNAi pathway using an shRNA-expression library. |
| title_short | Determination of the role of DDX3 a factor involved in mammalian RNAi pathway using an shRNA-expression library. |
| title_sort | determination of the role of ddx3 a factor involved in mammalian rnai pathway using an shrna expression library |
| url | http://europepmc.org/articles/PMC3602433?pdf=render |
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