Three-dimensional remodeling of collagen fibers within cervical tissues in pregnancy
The cervix is a collagen-rich connective tissue that must remain closed during pregnancy while undergoing progressive remodeling in preparation for delivery, which begins before the onset of the preterm labor process. Therefore, it is important to resolve the changes of collagen fibers during cervic...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
World Scientific Publishing
2023-07-01
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| Series: | Journal of Innovative Optical Health Sciences |
| Subjects: | |
| Online Access: | https://www.worldscientific.com/doi/10.1142/S1793545822430052 |
| _version_ | 1797777288076460032 |
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| author | Lingxi Zhou Rushan Jiang Jia Meng Shuhao Qian Shenyi Jiang Chuncheng Wang Chen Yang Zhihua Ding Zheyue Shu Zhiyi Liu |
| author_facet | Lingxi Zhou Rushan Jiang Jia Meng Shuhao Qian Shenyi Jiang Chuncheng Wang Chen Yang Zhihua Ding Zheyue Shu Zhiyi Liu |
| author_sort | Lingxi Zhou |
| collection | DOAJ |
| description | The cervix is a collagen-rich connective tissue that must remain closed during pregnancy while undergoing progressive remodeling in preparation for delivery, which begins before the onset of the preterm labor process. Therefore, it is important to resolve the changes of collagen fibers during cervical remodeling for the prevention of preterm labor. Herein, we assessed the spatial organization of collagen fibers in a three-dimensional (3D) context within cervical tissues of mice on day 3, 9, 12, 15 and 18 of gestation. We found that the 3D directional variance, a novel metric of alignment, was higher on day 9 than that on day 3 and then gradually decreased from day 9 to day 18. Compared with two-dimensional (2D) approach, a higher sensitivity was achieved from 3D analysis, highlighting the importance of truly 3D quantification. Moreover, the depth-dependent variation of 3D directional variance was investigated. By combining multiple 3D directional variance-derived metrics, a high level of classification accuracy was acquired in distinguishing different periods of pregnancy. These results demonstrate that 3D directional variance is sensitive to remodeling of collagen fibers within cervical tissues, shedding new light on highly-sensitive, early detection of preterm birth (PTB). |
| first_indexed | 2024-03-12T23:01:57Z |
| format | Article |
| id | doaj.art-93b9b48987484db7ae54bc34bb47acea |
| institution | Directory Open Access Journal |
| issn | 1793-5458 1793-7205 |
| language | English |
| last_indexed | 2024-03-12T23:01:57Z |
| publishDate | 2023-07-01 |
| publisher | World Scientific Publishing |
| record_format | Article |
| series | Journal of Innovative Optical Health Sciences |
| spelling | doaj.art-93b9b48987484db7ae54bc34bb47acea2023-07-19T08:37:30ZengWorld Scientific PublishingJournal of Innovative Optical Health Sciences1793-54581793-72052023-07-01160410.1142/S1793545822430052Three-dimensional remodeling of collagen fibers within cervical tissues in pregnancyLingxi Zhou0Rushan Jiang1Jia Meng2Shuhao Qian3Shenyi Jiang4Chuncheng Wang5Chen Yang6Zhihua Ding7Zheyue Shu8Zhiyi Liu9State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou, Zhejiang 310027, P. R. ChinaState Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou, Zhejiang 310027, P. R. ChinaState Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou, Zhejiang 310027, P. R. ChinaState Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou, Zhejiang 310027, P. R. ChinaState Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou, Zhejiang 310027, P. R. ChinaState Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou, Zhejiang 310027, P. R. ChinaState Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou, Zhejiang 310027, P. R. ChinaState Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou, Zhejiang 310027, P. R. ChinaDivision of Hepatobiliary and Pancreatic Surgery, Department of Surgery, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310027 P. R. ChinaState Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, International Research Center for Advanced Photonics, Zhejiang University, Hangzhou, Zhejiang 310027, P. R. ChinaThe cervix is a collagen-rich connective tissue that must remain closed during pregnancy while undergoing progressive remodeling in preparation for delivery, which begins before the onset of the preterm labor process. Therefore, it is important to resolve the changes of collagen fibers during cervical remodeling for the prevention of preterm labor. Herein, we assessed the spatial organization of collagen fibers in a three-dimensional (3D) context within cervical tissues of mice on day 3, 9, 12, 15 and 18 of gestation. We found that the 3D directional variance, a novel metric of alignment, was higher on day 9 than that on day 3 and then gradually decreased from day 9 to day 18. Compared with two-dimensional (2D) approach, a higher sensitivity was achieved from 3D analysis, highlighting the importance of truly 3D quantification. Moreover, the depth-dependent variation of 3D directional variance was investigated. By combining multiple 3D directional variance-derived metrics, a high level of classification accuracy was acquired in distinguishing different periods of pregnancy. These results demonstrate that 3D directional variance is sensitive to remodeling of collagen fibers within cervical tissues, shedding new light on highly-sensitive, early detection of preterm birth (PTB).https://www.worldscientific.com/doi/10.1142/S1793545822430052Preterm birthcervical tissuedirectional variancecollagen fiber3D analysis |
| spellingShingle | Lingxi Zhou Rushan Jiang Jia Meng Shuhao Qian Shenyi Jiang Chuncheng Wang Chen Yang Zhihua Ding Zheyue Shu Zhiyi Liu Three-dimensional remodeling of collagen fibers within cervical tissues in pregnancy Journal of Innovative Optical Health Sciences Preterm birth cervical tissue directional variance collagen fiber 3D analysis |
| title | Three-dimensional remodeling of collagen fibers within cervical tissues in pregnancy |
| title_full | Three-dimensional remodeling of collagen fibers within cervical tissues in pregnancy |
| title_fullStr | Three-dimensional remodeling of collagen fibers within cervical tissues in pregnancy |
| title_full_unstemmed | Three-dimensional remodeling of collagen fibers within cervical tissues in pregnancy |
| title_short | Three-dimensional remodeling of collagen fibers within cervical tissues in pregnancy |
| title_sort | three dimensional remodeling of collagen fibers within cervical tissues in pregnancy |
| topic | Preterm birth cervical tissue directional variance collagen fiber 3D analysis |
| url | https://www.worldscientific.com/doi/10.1142/S1793545822430052 |
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