Efficiency of Hydroxycinnamic Phenolic Acids to Inhibit the Production of Ochratoxin A by <i>Aspergillus westerdijkiae</i> and <i>Penicillium verrucosum</i>
Ochratoxin A (OTA) is one of the worldwide most important mycotoxins in terms of health and agroeconomic consequences. With the aim to promote the use of phytochemicals as alternatives to synthetic fungicides, the effect of hydroxycinnamic acids on the fungal growth and OTA yield by two major OTA-pr...
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MDPI AG
2020-11-01
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| Series: | International Journal of Molecular Sciences |
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| author | Saranyaphat Boonmee Vessela Atanasova Sylvain Chéreau Gisèle Marchegay Kevin D. Hyde Florence Richard-Forget |
| author_facet | Saranyaphat Boonmee Vessela Atanasova Sylvain Chéreau Gisèle Marchegay Kevin D. Hyde Florence Richard-Forget |
| author_sort | Saranyaphat Boonmee |
| collection | DOAJ |
| description | Ochratoxin A (OTA) is one of the worldwide most important mycotoxins in terms of health and agroeconomic consequences. With the aim to promote the use of phytochemicals as alternatives to synthetic fungicides, the effect of hydroxycinnamic acids on the fungal growth and OTA yield by two major OTA-producing species was investigated. After a first step dedicated to the definition of most suitable culture conditions, the impact of 0.5 mM ferulic (FER), <i>p</i>-coumaric (COUM), caffeic and chlorogenic acids was evaluated on <i>Aspergillus westerdijkiae</i> and <i>Penicillium verrucosum</i>. Whereas no fungal growth reduction was observed regardless of the phenolic acid and fungal isolate, our results demonstrated the capacity of FER and COUM to inhibit OTA production. The most efficient compound was FER that led to a 70% reduction of OTA yielded by <i>P. verrucosum</i> and, although not statistically significant, a 35% inhibition of OTA produced by <i>A. westerdijkiae</i>. To further investigate the bioactivity of FER and COUM, their metabolic fate was characterized in fungal broths. The capacity of <i>P. verrucosum</i> to metabolize FER and COUM through a C<sub>2</sub>-clivage type degradation was demonstrated. Overall, our data support the potential use of FER to prevent OTA contamination and reduce the use of synthetic pesticides. |
| first_indexed | 2024-03-10T14:53:01Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T14:53:01Z |
| publishDate | 2020-11-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-93bba23dae104501888c22f0dd972d982023-11-20T20:48:51ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-11-012122854810.3390/ijms21228548Efficiency of Hydroxycinnamic Phenolic Acids to Inhibit the Production of Ochratoxin A by <i>Aspergillus westerdijkiae</i> and <i>Penicillium verrucosum</i>Saranyaphat Boonmee0Vessela Atanasova1Sylvain Chéreau2Gisèle Marchegay3Kevin D. Hyde4Florence Richard-Forget5Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, ThailandUR1264 Mycology and Food Safety Research Unit (MycSA), INRAE Research Centre, F-22882 Villenave d’Ornon, FranceUR1264 Mycology and Food Safety Research Unit (MycSA), INRAE Research Centre, F-22882 Villenave d’Ornon, FranceUR1264 Mycology and Food Safety Research Unit (MycSA), INRAE Research Centre, F-22882 Villenave d’Ornon, FranceCenter of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, ThailandUR1264 Mycology and Food Safety Research Unit (MycSA), INRAE Research Centre, F-22882 Villenave d’Ornon, FranceOchratoxin A (OTA) is one of the worldwide most important mycotoxins in terms of health and agroeconomic consequences. With the aim to promote the use of phytochemicals as alternatives to synthetic fungicides, the effect of hydroxycinnamic acids on the fungal growth and OTA yield by two major OTA-producing species was investigated. After a first step dedicated to the definition of most suitable culture conditions, the impact of 0.5 mM ferulic (FER), <i>p</i>-coumaric (COUM), caffeic and chlorogenic acids was evaluated on <i>Aspergillus westerdijkiae</i> and <i>Penicillium verrucosum</i>. Whereas no fungal growth reduction was observed regardless of the phenolic acid and fungal isolate, our results demonstrated the capacity of FER and COUM to inhibit OTA production. The most efficient compound was FER that led to a 70% reduction of OTA yielded by <i>P. verrucosum</i> and, although not statistically significant, a 35% inhibition of OTA produced by <i>A. westerdijkiae</i>. To further investigate the bioactivity of FER and COUM, their metabolic fate was characterized in fungal broths. The capacity of <i>P. verrucosum</i> to metabolize FER and COUM through a C<sub>2</sub>-clivage type degradation was demonstrated. Overall, our data support the potential use of FER to prevent OTA contamination and reduce the use of synthetic pesticides.https://www.mdpi.com/1422-0067/21/22/8548Aspergillus westerdijkiaePenicillium verrucosumochratoxin Aphenolic acids |
| spellingShingle | Saranyaphat Boonmee Vessela Atanasova Sylvain Chéreau Gisèle Marchegay Kevin D. Hyde Florence Richard-Forget Efficiency of Hydroxycinnamic Phenolic Acids to Inhibit the Production of Ochratoxin A by <i>Aspergillus westerdijkiae</i> and <i>Penicillium verrucosum</i> International Journal of Molecular Sciences Aspergillus westerdijkiae Penicillium verrucosum ochratoxin A phenolic acids |
| title | Efficiency of Hydroxycinnamic Phenolic Acids to Inhibit the Production of Ochratoxin A by <i>Aspergillus westerdijkiae</i> and <i>Penicillium verrucosum</i> |
| title_full | Efficiency of Hydroxycinnamic Phenolic Acids to Inhibit the Production of Ochratoxin A by <i>Aspergillus westerdijkiae</i> and <i>Penicillium verrucosum</i> |
| title_fullStr | Efficiency of Hydroxycinnamic Phenolic Acids to Inhibit the Production of Ochratoxin A by <i>Aspergillus westerdijkiae</i> and <i>Penicillium verrucosum</i> |
| title_full_unstemmed | Efficiency of Hydroxycinnamic Phenolic Acids to Inhibit the Production of Ochratoxin A by <i>Aspergillus westerdijkiae</i> and <i>Penicillium verrucosum</i> |
| title_short | Efficiency of Hydroxycinnamic Phenolic Acids to Inhibit the Production of Ochratoxin A by <i>Aspergillus westerdijkiae</i> and <i>Penicillium verrucosum</i> |
| title_sort | efficiency of hydroxycinnamic phenolic acids to inhibit the production of ochratoxin a by i aspergillus westerdijkiae i and i penicillium verrucosum i |
| topic | Aspergillus westerdijkiae Penicillium verrucosum ochratoxin A phenolic acids |
| url | https://www.mdpi.com/1422-0067/21/22/8548 |
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