Voided Urinary Microbiota Is Stable Over Time but Impacted by Post Void Storage
Background: New sensitive techniques have revealed a thriving bacterial community in the human urinary tract, challenging the perception that urine in healthy humans is sterile. While the functional role of this urinary microbiota is unknown, dysbiosis has been linked to urgency urinary incontinence...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-08-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fcimb.2020.00435/full |
| _version_ | 1818478153250635776 |
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| author | Caspar Bundgaard-Nielsen Caspar Bundgaard-Nielsen Nadia Ammitzbøll Nadia Ammitzbøll Yusuf Abdi Isse Yusuf Abdi Isse Abdisalam Muqtar Abdisalam Muqtar Ann-Maria Jensen Peter D. C. Leutscher Peter D. C. Leutscher Peter D. C. Leutscher Louise Thomsen Schmidt Arenholt Louise Thomsen Schmidt Arenholt Louise Thomsen Schmidt Arenholt Søren Hagstrøm Søren Hagstrøm Søren Hagstrøm Søren Hagstrøm Suzette Sørensen Suzette Sørensen Suzette Sørensen |
| author_facet | Caspar Bundgaard-Nielsen Caspar Bundgaard-Nielsen Nadia Ammitzbøll Nadia Ammitzbøll Yusuf Abdi Isse Yusuf Abdi Isse Abdisalam Muqtar Abdisalam Muqtar Ann-Maria Jensen Peter D. C. Leutscher Peter D. C. Leutscher Peter D. C. Leutscher Louise Thomsen Schmidt Arenholt Louise Thomsen Schmidt Arenholt Louise Thomsen Schmidt Arenholt Søren Hagstrøm Søren Hagstrøm Søren Hagstrøm Søren Hagstrøm Suzette Sørensen Suzette Sørensen Suzette Sørensen |
| author_sort | Caspar Bundgaard-Nielsen |
| collection | DOAJ |
| description | Background: New sensitive techniques have revealed a thriving bacterial community in the human urinary tract, challenging the perception that urine in healthy humans is sterile. While the functional role of this urinary microbiota is unknown, dysbiosis has been linked to urgency urinary incontinence and risk of urinary tract infections. When comparing studies, it is crucial to account for possible confounders introduced due to methodological differences. Here we investigated whether collection and storage conditions had any impact on the urinary microbial composition.Results: For comparison of different storage conditions, midstream urine was collected from five healthy adult female donors and analyzed by 16S rRNA gene sequencing. Samples stored at −80 and −20°C, but not 4°C, were found to be comparable to freshly handled voided urine. Using the same methods, the daily or day-to-day variation in urinary microbiota was investigated in 19 healthy donors, including four women, five men, five girls, and five boys. Apart from two male adult donors, none of the tested conditions gave rise to significant differences in alpha and beta diversities between individuals.Conclusion: The composition of voided urinary microbiota was found to be effectively maintained by freezing, but not storage at 4°C. In addition, we did not observe any intrapersonal daily or day-to-day variations in microbiota composition in women, girls or boys. Together our study supports present methodologies that can be used in future studies investigating the urinary microbiota. |
| first_indexed | 2024-12-10T09:44:46Z |
| format | Article |
| id | doaj.art-93bcbded8efb4b938cfdd9ae17bd89c5 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-12-10T09:44:46Z |
| publishDate | 2020-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-93bcbded8efb4b938cfdd9ae17bd89c52022-12-22T01:53:52ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882020-08-011010.3389/fcimb.2020.00435533858Voided Urinary Microbiota Is Stable Over Time but Impacted by Post Void StorageCaspar Bundgaard-Nielsen0Caspar Bundgaard-Nielsen1Nadia Ammitzbøll2Nadia Ammitzbøll3Yusuf Abdi Isse4Yusuf Abdi Isse5Abdisalam Muqtar6Abdisalam Muqtar7Ann-Maria Jensen8Peter D. C. Leutscher9Peter D. C. Leutscher10Peter D. C. Leutscher11Louise Thomsen Schmidt Arenholt12Louise Thomsen Schmidt Arenholt13Louise Thomsen Schmidt Arenholt14Søren Hagstrøm15Søren Hagstrøm16Søren Hagstrøm17Søren Hagstrøm18Suzette Sørensen19Suzette Sørensen20Suzette Sørensen21Centre for Clinical Research, North Denmark Regional Hospital, Hjørring, DenmarkDepartment of Clinical Medicine, Aalborg University, Aalborg, DenmarkCentre for Clinical Research, North Denmark Regional Hospital, Hjørring, DenmarkDepartment of Clinical Medicine, Aalborg University, Aalborg, DenmarkCentre for Clinical Research, North Denmark Regional Hospital, Hjørring, DenmarkDepartment of Health Science and Technology, Aalborg University, Aalborg, DenmarkCentre for Clinical Research, North Denmark Regional Hospital, Hjørring, DenmarkDepartment of Health Science and Technology, Aalborg University, Aalborg, DenmarkCentre for Clinical Research, North Denmark Regional Hospital, Hjørring, DenmarkCentre for Clinical Research, North Denmark Regional Hospital, Hjørring, DenmarkDepartment of Clinical Medicine, Aalborg University, Aalborg, DenmarkSteno Diabetes Center North Jutland, Aalborg, DenmarkCentre for Clinical Research, North Denmark Regional Hospital, Hjørring, DenmarkDepartment of Clinical Medicine, Aalborg University, Aalborg, DenmarkDepartment of Gynecology and Obstetrics, North Denmark Regional Hospital, Hjørring, DenmarkCentre for Clinical Research, North Denmark Regional Hospital, Hjørring, DenmarkDepartment of Clinical Medicine, Aalborg University, Aalborg, DenmarkSteno Diabetes Center North Jutland, Aalborg, DenmarkDepartment of Pediatrics, Aalborg University Hospital, Aalborg, DenmarkCentre for Clinical Research, North Denmark Regional Hospital, Hjørring, DenmarkDepartment of Clinical Medicine, Aalborg University, Aalborg, DenmarkSteno Diabetes Center North Jutland, Aalborg, DenmarkBackground: New sensitive techniques have revealed a thriving bacterial community in the human urinary tract, challenging the perception that urine in healthy humans is sterile. While the functional role of this urinary microbiota is unknown, dysbiosis has been linked to urgency urinary incontinence and risk of urinary tract infections. When comparing studies, it is crucial to account for possible confounders introduced due to methodological differences. Here we investigated whether collection and storage conditions had any impact on the urinary microbial composition.Results: For comparison of different storage conditions, midstream urine was collected from five healthy adult female donors and analyzed by 16S rRNA gene sequencing. Samples stored at −80 and −20°C, but not 4°C, were found to be comparable to freshly handled voided urine. Using the same methods, the daily or day-to-day variation in urinary microbiota was investigated in 19 healthy donors, including four women, five men, five girls, and five boys. Apart from two male adult donors, none of the tested conditions gave rise to significant differences in alpha and beta diversities between individuals.Conclusion: The composition of voided urinary microbiota was found to be effectively maintained by freezing, but not storage at 4°C. In addition, we did not observe any intrapersonal daily or day-to-day variations in microbiota composition in women, girls or boys. Together our study supports present methodologies that can be used in future studies investigating the urinary microbiota.https://www.frontiersin.org/article/10.3389/fcimb.2020.00435/fullbacteriaDNA extractionmicrobiomemicrobiotaspecimen handlingurine |
| spellingShingle | Caspar Bundgaard-Nielsen Caspar Bundgaard-Nielsen Nadia Ammitzbøll Nadia Ammitzbøll Yusuf Abdi Isse Yusuf Abdi Isse Abdisalam Muqtar Abdisalam Muqtar Ann-Maria Jensen Peter D. C. Leutscher Peter D. C. Leutscher Peter D. C. Leutscher Louise Thomsen Schmidt Arenholt Louise Thomsen Schmidt Arenholt Louise Thomsen Schmidt Arenholt Søren Hagstrøm Søren Hagstrøm Søren Hagstrøm Søren Hagstrøm Suzette Sørensen Suzette Sørensen Suzette Sørensen Voided Urinary Microbiota Is Stable Over Time but Impacted by Post Void Storage Frontiers in Cellular and Infection Microbiology bacteria DNA extraction microbiome microbiota specimen handling urine |
| title | Voided Urinary Microbiota Is Stable Over Time but Impacted by Post Void Storage |
| title_full | Voided Urinary Microbiota Is Stable Over Time but Impacted by Post Void Storage |
| title_fullStr | Voided Urinary Microbiota Is Stable Over Time but Impacted by Post Void Storage |
| title_full_unstemmed | Voided Urinary Microbiota Is Stable Over Time but Impacted by Post Void Storage |
| title_short | Voided Urinary Microbiota Is Stable Over Time but Impacted by Post Void Storage |
| title_sort | voided urinary microbiota is stable over time but impacted by post void storage |
| topic | bacteria DNA extraction microbiome microbiota specimen handling urine |
| url | https://www.frontiersin.org/article/10.3389/fcimb.2020.00435/full |
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