Antiproliferative activity and apoptosis-inducing mechanism of Curcuma longa (Turmimax®) on HeLa cell lines
Abstract Curcumin, the primary polyphenol found in turmeric, is derived from the Curcuma longa plant. Since curcumin is nontoxic and has a wide range of medicinal qualities, including anti-oxidant, analgesic, anti-inflammatory, and antibacterial action, it has been widely employed in Ayurveda medici...
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| Format: | Article |
| Language: | English |
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Instituto Internacional de Ecologia
2023-10-01
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| Series: | Brazilian Journal of Biology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842023000100859&lng=en&tlng=en |
| _version_ | 1797662812006252544 |
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| author | H. M. Firoz S. Nanjundaiah C. T. Sadashiva B. Neethumol Y. Rashmi A. K. Sreedrisya |
| author_facet | H. M. Firoz S. Nanjundaiah C. T. Sadashiva B. Neethumol Y. Rashmi A. K. Sreedrisya |
| author_sort | H. M. Firoz |
| collection | DOAJ |
| description | Abstract Curcumin, the primary polyphenol found in turmeric, is derived from the Curcuma longa plant. Since curcumin is nontoxic and has a wide range of medicinal qualities, including anti-oxidant, analgesic, anti-inflammatory, and antibacterial action, it has been widely employed in Ayurveda medicine for ages. Curcumin has recently been discovered to have anti-cancer properties through its impact on numerous biological pathways involved in carcinogenesis, metastasis, tumorigenesis, cell cycle regulation, mutagenesis, and oncogene expression. In this study, we determined the Antiproliferative activity and apoptosis-inducing mechanism of C. longa (Turmimax®) on human cancer cells. The cytotoxic effect was evaluated against HeLa cell lines using the MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay. Flow cytometric analysis was performed to detect apoptotic cell death. Turmimax® exhibits promising properties as a potential anti-cancer therapeutic agent in human cervical adenocarcinomas and possibly other cancer types, with an IC50 value of 87.89 µg/mL. In HeLa cells treated with Turmimax®, cell cycle arrest was seen in the G0/G1 and S phases. By inducing apoptosis and increasing the number of apoptotic cells in a dose-dependent manner, the experimental data suggest that Turmimax® has considerable promise in cancer prevention and treatment. |
| first_indexed | 2024-03-11T19:05:30Z |
| format | Article |
| id | doaj.art-93bd747615de4c6f8a39fdb3004aedcf |
| institution | Directory Open Access Journal |
| issn | 1678-4375 |
| language | English |
| last_indexed | 2024-03-11T19:05:30Z |
| publishDate | 2023-10-01 |
| publisher | Instituto Internacional de Ecologia |
| record_format | Article |
| series | Brazilian Journal of Biology |
| spelling | doaj.art-93bd747615de4c6f8a39fdb3004aedcf2023-10-10T07:45:08ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-43752023-10-018310.1590/1519-6984.275953Antiproliferative activity and apoptosis-inducing mechanism of Curcuma longa (Turmimax®) on HeLa cell linesH. M. FirozS. NanjundaiahC. T. Sadashivahttps://orcid.org/0000-0002-0276-2792B. NeethumolY. RashmiA. K. SreedrisyaAbstract Curcumin, the primary polyphenol found in turmeric, is derived from the Curcuma longa plant. Since curcumin is nontoxic and has a wide range of medicinal qualities, including anti-oxidant, analgesic, anti-inflammatory, and antibacterial action, it has been widely employed in Ayurveda medicine for ages. Curcumin has recently been discovered to have anti-cancer properties through its impact on numerous biological pathways involved in carcinogenesis, metastasis, tumorigenesis, cell cycle regulation, mutagenesis, and oncogene expression. In this study, we determined the Antiproliferative activity and apoptosis-inducing mechanism of C. longa (Turmimax®) on human cancer cells. The cytotoxic effect was evaluated against HeLa cell lines using the MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay. Flow cytometric analysis was performed to detect apoptotic cell death. Turmimax® exhibits promising properties as a potential anti-cancer therapeutic agent in human cervical adenocarcinomas and possibly other cancer types, with an IC50 value of 87.89 µg/mL. In HeLa cells treated with Turmimax®, cell cycle arrest was seen in the G0/G1 and S phases. By inducing apoptosis and increasing the number of apoptotic cells in a dose-dependent manner, the experimental data suggest that Turmimax® has considerable promise in cancer prevention and treatment.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842023000100859&lng=en&tlng=enantiproliferativeCurcuma longaHeLa cell linesapoptosis |
| spellingShingle | H. M. Firoz S. Nanjundaiah C. T. Sadashiva B. Neethumol Y. Rashmi A. K. Sreedrisya Antiproliferative activity and apoptosis-inducing mechanism of Curcuma longa (Turmimax®) on HeLa cell lines Brazilian Journal of Biology antiproliferative Curcuma longa HeLa cell lines apoptosis |
| title | Antiproliferative activity and apoptosis-inducing mechanism of Curcuma longa (Turmimax®) on HeLa cell lines |
| title_full | Antiproliferative activity and apoptosis-inducing mechanism of Curcuma longa (Turmimax®) on HeLa cell lines |
| title_fullStr | Antiproliferative activity and apoptosis-inducing mechanism of Curcuma longa (Turmimax®) on HeLa cell lines |
| title_full_unstemmed | Antiproliferative activity and apoptosis-inducing mechanism of Curcuma longa (Turmimax®) on HeLa cell lines |
| title_short | Antiproliferative activity and apoptosis-inducing mechanism of Curcuma longa (Turmimax®) on HeLa cell lines |
| title_sort | antiproliferative activity and apoptosis inducing mechanism of curcuma longa turmimax r on hela cell lines |
| topic | antiproliferative Curcuma longa HeLa cell lines apoptosis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842023000100859&lng=en&tlng=en |
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