Protective effect of different antioxidant agents in UVB-irradiated keratinocytes
<p>Skin cells can respond to UVB-induced damage either by tolerating it, or restoring it through antioxidant activation and DNA repair mechanisms or, ultimately, undergoing programmed cell death, when damage is massive. Nutritional factors, in particular, food antioxidants, have attracted much...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
PAGEPress Publications
2017-09-01
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| Series: | European Journal of Histochemistry |
| Subjects: | |
| Online Access: | http://www.ejh.it/index.php/ejh/article/view/2784 |
| _version_ | 1819121449682599936 |
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| author | Sara Salucci Sabrina Burattini Francesca Buontempo Alberto Maria Martelli Elisabetta Falcieri Michela Battistelli |
| author_facet | Sara Salucci Sabrina Burattini Francesca Buontempo Alberto Maria Martelli Elisabetta Falcieri Michela Battistelli |
| author_sort | Sara Salucci |
| collection | DOAJ |
| description | <p>Skin cells can respond to UVB-induced damage either by tolerating it, or restoring it through antioxidant activation and DNA repair mechanisms or, ultimately, undergoing programmed cell death, when damage is massive. Nutritional factors, in particular, food antioxidants, have attracted much interest because of their potential use in new preventive, protective, and therapeutic strategies for chronic degenerative diseases, including skin inflammation and cancer. Some polyphenols, present in virgin olive oil, well tolerated by organism after oral administration, show a variety of pharmacological and clinical benefits such as anti-oxidant, anti-cancer, anti-inflammatory, and neuro-protective activities. Here, the protective effects of antioxidant compounds against UV-induced apoptosis have been described in HaCat cell line. Human keratinocytes were pre-treated with antioxidants before UVB exposure and their effects have been evaluated by means of ultrastructural analyses. After UVB radiation, a known cell death trigger, typical apoptotic features, absent in control condition and in antioxidant alone-treated cells, appear. An evident numerical decrease of ultrastructural apoptotic patterns and TUNEL positive nuclei can be observed when natural antioxidants were supplied before cell death induction. These data have been confirmed by molecular investigation of caspase activity. In conclusion, this paper highlights antioxidant compound ability to prevent apoptotic cell death in human keratinocytes exposed to UVB, suggesting, for these molecules, a potential role in preventing skin damage. </p> |
| first_indexed | 2024-12-22T06:36:44Z |
| format | Article |
| id | doaj.art-93bde68f5e384d35b22ede221b29bceb |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-12-22T06:36:44Z |
| publishDate | 2017-09-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-93bde68f5e384d35b22ede221b29bceb2022-12-21T18:35:34ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062017-09-0161310.4081/ejh.2017.27841575Protective effect of different antioxidant agents in UVB-irradiated keratinocytesSara Salucci0Sabrina Burattini1Francesca Buontempo2Alberto Maria Martelli3Elisabetta Falcieri4Michela Battistelli5University of Urbino “Carlo Bo”University of Urbino “Carlo Bo”University of BolognaUniversity of BolognaUniversity of Urbino “Carlo Bo”University of Urbino “Carlo Bo”<p>Skin cells can respond to UVB-induced damage either by tolerating it, or restoring it through antioxidant activation and DNA repair mechanisms or, ultimately, undergoing programmed cell death, when damage is massive. Nutritional factors, in particular, food antioxidants, have attracted much interest because of their potential use in new preventive, protective, and therapeutic strategies for chronic degenerative diseases, including skin inflammation and cancer. Some polyphenols, present in virgin olive oil, well tolerated by organism after oral administration, show a variety of pharmacological and clinical benefits such as anti-oxidant, anti-cancer, anti-inflammatory, and neuro-protective activities. Here, the protective effects of antioxidant compounds against UV-induced apoptosis have been described in HaCat cell line. Human keratinocytes were pre-treated with antioxidants before UVB exposure and their effects have been evaluated by means of ultrastructural analyses. After UVB radiation, a known cell death trigger, typical apoptotic features, absent in control condition and in antioxidant alone-treated cells, appear. An evident numerical decrease of ultrastructural apoptotic patterns and TUNEL positive nuclei can be observed when natural antioxidants were supplied before cell death induction. These data have been confirmed by molecular investigation of caspase activity. In conclusion, this paper highlights antioxidant compound ability to prevent apoptotic cell death in human keratinocytes exposed to UVB, suggesting, for these molecules, a potential role in preventing skin damage. </p>http://www.ejh.it/index.php/ejh/article/view/2784KeratinocytesUVB radiationnatural antioxidantsultrastructureTUNEL. |
| spellingShingle | Sara Salucci Sabrina Burattini Francesca Buontempo Alberto Maria Martelli Elisabetta Falcieri Michela Battistelli Protective effect of different antioxidant agents in UVB-irradiated keratinocytes European Journal of Histochemistry Keratinocytes UVB radiation natural antioxidants ultrastructure TUNEL. |
| title | Protective effect of different antioxidant agents in UVB-irradiated keratinocytes |
| title_full | Protective effect of different antioxidant agents in UVB-irradiated keratinocytes |
| title_fullStr | Protective effect of different antioxidant agents in UVB-irradiated keratinocytes |
| title_full_unstemmed | Protective effect of different antioxidant agents in UVB-irradiated keratinocytes |
| title_short | Protective effect of different antioxidant agents in UVB-irradiated keratinocytes |
| title_sort | protective effect of different antioxidant agents in uvb irradiated keratinocytes |
| topic | Keratinocytes UVB radiation natural antioxidants ultrastructure TUNEL. |
| url | http://www.ejh.it/index.php/ejh/article/view/2784 |
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