Rotaviral Diarrhoea in Children: A Comparison of PAGE with ELISA
Background: Rotavirus infects almost all children by the age of five. More than 150,000 annual deaths due to rota virus, occurs in India. Aims: This study aimed to determine the incidence of Rotavirus infection in children and compare enzyme - linked immunosorbent assay (ELISA) and a modified po...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2012-04-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/2004/10-%203907.A.pdf |
| Summary: | Background: Rotavirus infects almost all children by the age of five.
More than 150,000 annual deaths due to rota virus, occurs in India.
Aims: This study aimed to determine the incidence of Rotavirus
infection in children and compare enzyme - linked immunosorbent
assay (ELISA) and a modified polyacrylamide gel elctrophoretic
(PAGE) analysis in detection of rota virus in stool samples.
Materials and Methods: In this prospective study, a total of 200
stool samples were examined for the presence of Rota virus by
ELISA and by a modified PAGE analysis of viral genome. Stool
culture was done for common enteric pathogens.
Results: Maximum incidence of rotavirus infection was seen in
age group of 6m-24 m(32.3%). Excellent correlation of ELISA
and PAGE results was found in194 of 200 (97%) specimens.
A total of 51 (25.5%) of them were found to be positive for
rotavirus by either methods. The proportion of ELISA +ve PAGE
–ve samples 1/200 was lower than the proportion of ELISA-ve
PAGE +ve samples (5/200). All 51 rotavirus positive cases did
not show infection with bacterial pathogens.
Conclusion: The modified PAGE technique for the detection
of viral RNA was found to be rapid, simple, reliable and less
expensive technique. |
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| ISSN: | 2249-782X 0973-709X |