Rotaviral Diarrhoea in Children: A Comparison of PAGE with ELISA
Background: Rotavirus infects almost all children by the age of five. More than 150,000 annual deaths due to rota virus, occurs in India. Aims: This study aimed to determine the incidence of Rotavirus infection in children and compare enzyme - linked immunosorbent assay (ELISA) and a modified po...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Private Limited
2012-04-01
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| Series: | Journal of Clinical and Diagnostic Research |
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| Online Access: | https://jcdr.net/articles/PDF/2004/10-%203907.A.pdf |
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| author | Venkatesh V.N. Prashanth H.V. K.G. Bhat Subha D.S. Sudhindra K.S. Farheen Fathima |
| author_facet | Venkatesh V.N. Prashanth H.V. K.G. Bhat Subha D.S. Sudhindra K.S. Farheen Fathima |
| author_sort | Venkatesh V.N. |
| collection | DOAJ |
| description | Background: Rotavirus infects almost all children by the age of five.
More than 150,000 annual deaths due to rota virus, occurs in India.
Aims: This study aimed to determine the incidence of Rotavirus
infection in children and compare enzyme - linked immunosorbent
assay (ELISA) and a modified polyacrylamide gel elctrophoretic
(PAGE) analysis in detection of rota virus in stool samples.
Materials and Methods: In this prospective study, a total of 200
stool samples were examined for the presence of Rota virus by
ELISA and by a modified PAGE analysis of viral genome. Stool
culture was done for common enteric pathogens.
Results: Maximum incidence of rotavirus infection was seen in
age group of 6m-24 m(32.3%). Excellent correlation of ELISA
and PAGE results was found in194 of 200 (97%) specimens.
A total of 51 (25.5%) of them were found to be positive for
rotavirus by either methods. The proportion of ELISA +ve PAGE
–ve samples 1/200 was lower than the proportion of ELISA-ve
PAGE +ve samples (5/200). All 51 rotavirus positive cases did
not show infection with bacterial pathogens.
Conclusion: The modified PAGE technique for the detection
of viral RNA was found to be rapid, simple, reliable and less
expensive technique. |
| first_indexed | 2024-12-13T11:31:39Z |
| format | Article |
| id | doaj.art-93c170aa69a54413852ca09b97714e7b |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-13T11:31:39Z |
| publishDate | 2012-04-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-93c170aa69a54413852ca09b97714e7b2022-12-21T23:47:54ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2012-04-0162188191Rotaviral Diarrhoea in Children: A Comparison of PAGE with ELISAVenkatesh V.N.0Prashanth H.V.1K.G. Bhat2Subha D.S.3Sudhindra K.S.4Farheen Fathima5Associate Professor, Dept of Microbiology, Basaweshwara Medical College, Chitradurga, Karnataka, India.Associate Professor, Dept of Microbiology, Sri Siddhartha Medical college, Agalakote, Tumkur, Karnataka, India.Professor and Head of Department, Maratha Mandal Dental College, Belgaum, Karnataka, India.Assistant Professor, Dept of Microbiology, Basaweshwara Medical College, Chitradurga, Karnataka, India. Assistant Professor, Dept of Microbiology, Basaweshwara Medical College, Chitradurga, Karnataka, India.Mrs Farheen Fathima, Assistant Professor, Dept of Microbiology, Basaweshwara Medical College, Chitradurga, Karnataka, India.Background: Rotavirus infects almost all children by the age of five. More than 150,000 annual deaths due to rota virus, occurs in India. Aims: This study aimed to determine the incidence of Rotavirus infection in children and compare enzyme - linked immunosorbent assay (ELISA) and a modified polyacrylamide gel elctrophoretic (PAGE) analysis in detection of rota virus in stool samples. Materials and Methods: In this prospective study, a total of 200 stool samples were examined for the presence of Rota virus by ELISA and by a modified PAGE analysis of viral genome. Stool culture was done for common enteric pathogens. Results: Maximum incidence of rotavirus infection was seen in age group of 6m-24 m(32.3%). Excellent correlation of ELISA and PAGE results was found in194 of 200 (97%) specimens. A total of 51 (25.5%) of them were found to be positive for rotavirus by either methods. The proportion of ELISA +ve PAGE –ve samples 1/200 was lower than the proportion of ELISA-ve PAGE +ve samples (5/200). All 51 rotavirus positive cases did not show infection with bacterial pathogens. Conclusion: The modified PAGE technique for the detection of viral RNA was found to be rapid, simple, reliable and less expensive technique.https://jcdr.net/articles/PDF/2004/10-%203907.A.pdfrotaviruspolyacrylamide gel electrophoresis (page) techniqueenzyme - linked immunosorbent assay (elisa)diahorrea |
| spellingShingle | Venkatesh V.N. Prashanth H.V. K.G. Bhat Subha D.S. Sudhindra K.S. Farheen Fathima Rotaviral Diarrhoea in Children: A Comparison of PAGE with ELISA Journal of Clinical and Diagnostic Research rotavirus polyacrylamide gel electrophoresis (page) technique enzyme - linked immunosorbent assay (elisa) diahorrea |
| title | Rotaviral Diarrhoea in Children: A Comparison of PAGE with ELISA |
| title_full | Rotaviral Diarrhoea in Children: A Comparison of PAGE with ELISA |
| title_fullStr | Rotaviral Diarrhoea in Children: A Comparison of PAGE with ELISA |
| title_full_unstemmed | Rotaviral Diarrhoea in Children: A Comparison of PAGE with ELISA |
| title_short | Rotaviral Diarrhoea in Children: A Comparison of PAGE with ELISA |
| title_sort | rotaviral diarrhoea in children a comparison of page with elisa |
| topic | rotavirus polyacrylamide gel electrophoresis (page) technique enzyme - linked immunosorbent assay (elisa) diahorrea |
| url | https://jcdr.net/articles/PDF/2004/10-%203907.A.pdf |
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