The interplay of inflammation and placenta in maternal diabetes: insights into Hofbauer cell expression patterns
IntroductionInflammation of the placenta is harmful to both the fetus and the mother. Inflammation is strongly associated with diabetes, a common complication of pregnancy. Hofbauer cells (HBCs), unique immune system cells of fetal origin in the placenta, play complex roles, including growth of plac...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1386528/full |
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| author | Zdenek Tauber Adela Burianova Katerina Koubova Max Mrstik Marie Jirkovska Katerina Cizkova |
| author_facet | Zdenek Tauber Adela Burianova Katerina Koubova Max Mrstik Marie Jirkovska Katerina Cizkova |
| author_sort | Zdenek Tauber |
| collection | DOAJ |
| description | IntroductionInflammation of the placenta is harmful to both the fetus and the mother. Inflammation is strongly associated with diabetes, a common complication of pregnancy. Hofbauer cells (HBCs), unique immune system cells of fetal origin in the placenta, play complex roles, including growth of placental villi and their branching, stromal remodelling, and angiogenesis.MethodsOur study investigated the expression of IL-1β, IL-10, CYP2C8, CYP2C9, CYP2J2 and sEH in HBCs from patients with type 1 diabetes mellitus (T1DM) and gestational diabetes mellitus (GDM) compared to healthy controls using immunohistochemistry. We also assessed the structure of the villus stroma using Masson´s trichrome.ResultsIn T1DM, HBCs showed inflammatory activation characterised by increased IL-1β and decreased CYP epoxygenase expression compared to normal placentas. Conversely, significant inflammation in HBCs appeared less likely in GDM, as levels of IL-1β and CYP epoxygenases remained stable compared to normal placentas. However, GDM showed a significant increase in sEH expression. Both types of diabetes showed delayed placental villous maturation and hypovascularisation, with GDM showing a more pronounced effect.ConclusionThe expression profiles of IL-1β, CYP epoxygenases and sEH significantlly differ between controls and diabetic placentas and between T1DM and GDM. These facts suggest an association of the CYP epoxygenase-EETs-sEH axis with IL-1β expression as well as villous stromal hypovascularisation. Given the stable high expression of IL-10 in both controls and both types of diabetes, it appears that immune tolerance is maintained in HBCs. |
| first_indexed | 2024-04-24T19:47:35Z |
| format | Article |
| id | doaj.art-93c2e762fe034c38b9dc47ed1ac14d48 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-24T19:47:35Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-93c2e762fe034c38b9dc47ed1ac14d482024-03-25T04:59:46ZengFrontiers Media S.A.Frontiers in Immunology1664-32242024-03-011510.3389/fimmu.2024.13865281386528The interplay of inflammation and placenta in maternal diabetes: insights into Hofbauer cell expression patternsZdenek Tauber0Adela Burianova1Katerina Koubova2Max Mrstik3Marie Jirkovska4Katerina Cizkova5Department of Histology and Embryology, Faculty of Medicine and Dentistry, Palacky University, Olomouc, CzechiaDepartment of Histology and Embryology, Faculty of Medicine and Dentistry, Palacky University, Olomouc, CzechiaDepartment of Histology and Embryology, Faculty of Medicine and Dentistry, Palacky University, Olomouc, CzechiaDepartment of Histology and Embryology, Faculty of Medicine and Dentistry, Palacky University, Olomouc, CzechiaInstitute of Histology and Embryology, First Faculty of Medicine, Charles University in Prague, Prague, CzechiaDepartment of Histology and Embryology, Faculty of Medicine and Dentistry, Palacky University, Olomouc, CzechiaIntroductionInflammation of the placenta is harmful to both the fetus and the mother. Inflammation is strongly associated with diabetes, a common complication of pregnancy. Hofbauer cells (HBCs), unique immune system cells of fetal origin in the placenta, play complex roles, including growth of placental villi and their branching, stromal remodelling, and angiogenesis.MethodsOur study investigated the expression of IL-1β, IL-10, CYP2C8, CYP2C9, CYP2J2 and sEH in HBCs from patients with type 1 diabetes mellitus (T1DM) and gestational diabetes mellitus (GDM) compared to healthy controls using immunohistochemistry. We also assessed the structure of the villus stroma using Masson´s trichrome.ResultsIn T1DM, HBCs showed inflammatory activation characterised by increased IL-1β and decreased CYP epoxygenase expression compared to normal placentas. Conversely, significant inflammation in HBCs appeared less likely in GDM, as levels of IL-1β and CYP epoxygenases remained stable compared to normal placentas. However, GDM showed a significant increase in sEH expression. Both types of diabetes showed delayed placental villous maturation and hypovascularisation, with GDM showing a more pronounced effect.ConclusionThe expression profiles of IL-1β, CYP epoxygenases and sEH significantlly differ between controls and diabetic placentas and between T1DM and GDM. These facts suggest an association of the CYP epoxygenase-EETs-sEH axis with IL-1β expression as well as villous stromal hypovascularisation. Given the stable high expression of IL-10 in both controls and both types of diabetes, it appears that immune tolerance is maintained in HBCs.https://www.frontiersin.org/articles/10.3389/fimmu.2024.1386528/fullhofbauer cellsplacental inflammationtype 1 diabetes mellitusgestational diabetes mellitusCYP epoxygenasessoluble epoxide hydrolase |
| spellingShingle | Zdenek Tauber Adela Burianova Katerina Koubova Max Mrstik Marie Jirkovska Katerina Cizkova The interplay of inflammation and placenta in maternal diabetes: insights into Hofbauer cell expression patterns Frontiers in Immunology hofbauer cells placental inflammation type 1 diabetes mellitus gestational diabetes mellitus CYP epoxygenases soluble epoxide hydrolase |
| title | The interplay of inflammation and placenta in maternal diabetes: insights into Hofbauer cell expression patterns |
| title_full | The interplay of inflammation and placenta in maternal diabetes: insights into Hofbauer cell expression patterns |
| title_fullStr | The interplay of inflammation and placenta in maternal diabetes: insights into Hofbauer cell expression patterns |
| title_full_unstemmed | The interplay of inflammation and placenta in maternal diabetes: insights into Hofbauer cell expression patterns |
| title_short | The interplay of inflammation and placenta in maternal diabetes: insights into Hofbauer cell expression patterns |
| title_sort | interplay of inflammation and placenta in maternal diabetes insights into hofbauer cell expression patterns |
| topic | hofbauer cells placental inflammation type 1 diabetes mellitus gestational diabetes mellitus CYP epoxygenases soluble epoxide hydrolase |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1386528/full |
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