Implementation of an in-house flow cytometric analysis of DNA fragmentation in spermatozoa
An increased amount of DNA fragmentation in the spermatozoa (SDF) is linked to male infertility. The Sperm Chromatin Structure Assay (SCSA) is widely used for analysis of SDF. However, the current software (SCSASoft®) linked to this assay is licensed and often located within larger diagnostic center...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2020-01-01
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| Series: | Asian Journal of Andrology |
| Subjects: | |
| Online Access: | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2020;volume=22;issue=3;spage=246;epage=251;aulast=Rex |
| _version_ | 1798045050897170432 |
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| author | Anne Sofie Rex Chunsen Wu Jørn Aagaard Jens Fedder |
| author_facet | Anne Sofie Rex Chunsen Wu Jørn Aagaard Jens Fedder |
| author_sort | Anne Sofie Rex |
| collection | DOAJ |
| description | An increased amount of DNA fragmentation in the spermatozoa (SDF) is linked to male infertility. The Sperm Chromatin Structure Assay (SCSA) is widely used for analysis of SDF. However, the current software (SCSASoft®) linked to this assay is licensed and often located within larger diagnostic centers. In this study, we present a protocol for using other types of software than SCSASoft® to determine the SDF index (DFI) with clinical relevance. This protocol is engineered after collecting and analyzing 254 samples from fertility patients and sperm donors over a 15-month period. DFI is analyzed using a strict protocol where the spermatozoa are treated with a strong acid (pH 1.2) followed by acridine orange. DFI is determined by a standard flow cytometric software, FACSDiva 6.1.3. Analysis of the outcome of the fertility treatment is included for 137 patients receiving either intrauterine inseminations (IUI) or timed coitus (TC). The results show that the chance of pregnancy declines as DFI increases. We also found that the male DFI affects the chance of pregnancy independent of the female age. We have shown that a standard flow cytometric software can be used when determining a clinical relevant DFI. These findings are a significant step toward implementing the analysis as a part of the routine, in-house diagnosing of the male fertility patient and subsequently optimizing the treatment course of the couple with reduced human and financial costs. |
| first_indexed | 2024-04-11T23:14:27Z |
| format | Article |
| id | doaj.art-93c994260cdd4520984b849d6411ad92 |
| institution | Directory Open Access Journal |
| issn | 1008-682X 1745-7262 |
| language | English |
| last_indexed | 2024-04-11T23:14:27Z |
| publishDate | 2020-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Journal of Andrology |
| spelling | doaj.art-93c994260cdd4520984b849d6411ad922022-12-22T03:57:40ZengWolters Kluwer Medknow PublicationsAsian Journal of Andrology1008-682X1745-72622020-01-0122324625110.4103/aja.aja_51_19Implementation of an in-house flow cytometric analysis of DNA fragmentation in spermatozoaAnne Sofie RexChunsen WuJørn AagaardJens FedderAn increased amount of DNA fragmentation in the spermatozoa (SDF) is linked to male infertility. The Sperm Chromatin Structure Assay (SCSA) is widely used for analysis of SDF. However, the current software (SCSASoft®) linked to this assay is licensed and often located within larger diagnostic centers. In this study, we present a protocol for using other types of software than SCSASoft® to determine the SDF index (DFI) with clinical relevance. This protocol is engineered after collecting and analyzing 254 samples from fertility patients and sperm donors over a 15-month period. DFI is analyzed using a strict protocol where the spermatozoa are treated with a strong acid (pH 1.2) followed by acridine orange. DFI is determined by a standard flow cytometric software, FACSDiva 6.1.3. Analysis of the outcome of the fertility treatment is included for 137 patients receiving either intrauterine inseminations (IUI) or timed coitus (TC). The results show that the chance of pregnancy declines as DFI increases. We also found that the male DFI affects the chance of pregnancy independent of the female age. We have shown that a standard flow cytometric software can be used when determining a clinical relevant DFI. These findings are a significant step toward implementing the analysis as a part of the routine, in-house diagnosing of the male fertility patient and subsequently optimizing the treatment course of the couple with reduced human and financial costs.http://www.ajandrology.com/article.asp?issn=1008-682X;year=2020;volume=22;issue=3;spage=246;epage=251;aulast=Rexdna fragmentation; flow cytometry; in-house analysis; intrauterine insemination; sperm chromatin structure assay; spermatozoa |
| spellingShingle | Anne Sofie Rex Chunsen Wu Jørn Aagaard Jens Fedder Implementation of an in-house flow cytometric analysis of DNA fragmentation in spermatozoa Asian Journal of Andrology dna fragmentation; flow cytometry; in-house analysis; intrauterine insemination; sperm chromatin structure assay; spermatozoa |
| title | Implementation of an in-house flow cytometric analysis of DNA fragmentation in spermatozoa |
| title_full | Implementation of an in-house flow cytometric analysis of DNA fragmentation in spermatozoa |
| title_fullStr | Implementation of an in-house flow cytometric analysis of DNA fragmentation in spermatozoa |
| title_full_unstemmed | Implementation of an in-house flow cytometric analysis of DNA fragmentation in spermatozoa |
| title_short | Implementation of an in-house flow cytometric analysis of DNA fragmentation in spermatozoa |
| title_sort | implementation of an in house flow cytometric analysis of dna fragmentation in spermatozoa |
| topic | dna fragmentation; flow cytometry; in-house analysis; intrauterine insemination; sperm chromatin structure assay; spermatozoa |
| url | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2020;volume=22;issue=3;spage=246;epage=251;aulast=Rex |
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