Global metabolic profiling of Arabidopsis Polyamine Oxidase 4 (AtPAO4) loss-of-function mutants exhibiting delayed dark-induced senescence
Early and more recent studies have suggested that some polyamines (PAs), and particularly spermine (Spm), exhibit anti-senescence properties in plants. In this work, we have investigated the role of Arabidopsis Polyamine Oxidase 4 (PAO4), encoding a PA back-conversion oxidase, during dark-induced se...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00173/full |
| _version_ | 1817979524229365760 |
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| author | Miren Iranzu Sequera-Mutiozabal Alexander eErban Joachim eKopka Kostadin Evgeniev eAtanasov Jaume eBastida Vasileios eFotopoulos Rubén eAlcázar Antonio F. Tiburcio |
| author_facet | Miren Iranzu Sequera-Mutiozabal Alexander eErban Joachim eKopka Kostadin Evgeniev eAtanasov Jaume eBastida Vasileios eFotopoulos Rubén eAlcázar Antonio F. Tiburcio |
| author_sort | Miren Iranzu Sequera-Mutiozabal |
| collection | DOAJ |
| description | Early and more recent studies have suggested that some polyamines (PAs), and particularly spermine (Spm), exhibit anti-senescence properties in plants. In this work, we have investigated the role of Arabidopsis Polyamine Oxidase 4 (PAO4), encoding a PA back-conversion oxidase, during dark-induced senescence. Two independent PAO4 (pao4-1 and pao4-2) loss-of-function mutants have been found that accumulate 10-fold higher Spm, and this associated with delayed entry into senescence under dark conditions. Mechanisms underlying pao4 delayed senescence have been studied using global metabolic profiling by GC-TOF/MS. pao4 mutants exhibit constitutively higher levels of important metabolites involved in redox regulation, central metabolism and signaling that support a priming status against oxidative stress. During senescence, interactions between PAs and oxidative, sugar and nitrogen metabolism have been detected that additively contribute to delayed entry into senescence. Our results indicate the occurrence of metabolic interactions between PAs, particularly Spm, with cell oxidative balance and transport/biosynthesis of amino acids as a strategy to cope with oxidative damage produced during senescence. |
| first_indexed | 2024-04-13T22:43:53Z |
| format | Article |
| id | doaj.art-93cdb9639ca143e89b96d5c6f84c180e |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T22:43:53Z |
| publishDate | 2016-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-93cdb9639ca143e89b96d5c6f84c180e2022-12-22T02:26:30ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-02-01710.3389/fpls.2016.00173178777Global metabolic profiling of Arabidopsis Polyamine Oxidase 4 (AtPAO4) loss-of-function mutants exhibiting delayed dark-induced senescenceMiren Iranzu Sequera-Mutiozabal0Alexander eErban1Joachim eKopka2Kostadin Evgeniev eAtanasov3Jaume eBastida4Vasileios eFotopoulos5Rubén eAlcázar6Antonio F. Tiburcio7University of Barcelona, Faculty of PharmacyMax-Planck-Institut für Molekulare PflanzenphysiologieMax-Planck-Institut für Molekulare PflanzenphysiologieUniversity of Barcelona, Faculty of PharmacyUniversity of Barcelona, Faculty of PharmacyCyprus University of TechnologyUniversity of Barcelona, Faculty of PharmacyUniversity of Barcelona, Faculty of PharmacyEarly and more recent studies have suggested that some polyamines (PAs), and particularly spermine (Spm), exhibit anti-senescence properties in plants. In this work, we have investigated the role of Arabidopsis Polyamine Oxidase 4 (PAO4), encoding a PA back-conversion oxidase, during dark-induced senescence. Two independent PAO4 (pao4-1 and pao4-2) loss-of-function mutants have been found that accumulate 10-fold higher Spm, and this associated with delayed entry into senescence under dark conditions. Mechanisms underlying pao4 delayed senescence have been studied using global metabolic profiling by GC-TOF/MS. pao4 mutants exhibit constitutively higher levels of important metabolites involved in redox regulation, central metabolism and signaling that support a priming status against oxidative stress. During senescence, interactions between PAs and oxidative, sugar and nitrogen metabolism have been detected that additively contribute to delayed entry into senescence. Our results indicate the occurrence of metabolic interactions between PAs, particularly Spm, with cell oxidative balance and transport/biosynthesis of amino acids as a strategy to cope with oxidative damage produced during senescence.http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00173/fullArabidopsisOxidative StressPolyaminesSperminesenescencePolyamine oxidase (PAO) |
| spellingShingle | Miren Iranzu Sequera-Mutiozabal Alexander eErban Joachim eKopka Kostadin Evgeniev eAtanasov Jaume eBastida Vasileios eFotopoulos Rubén eAlcázar Antonio F. Tiburcio Global metabolic profiling of Arabidopsis Polyamine Oxidase 4 (AtPAO4) loss-of-function mutants exhibiting delayed dark-induced senescence Frontiers in Plant Science Arabidopsis Oxidative Stress Polyamines Spermine senescence Polyamine oxidase (PAO) |
| title | Global metabolic profiling of Arabidopsis Polyamine Oxidase 4 (AtPAO4) loss-of-function mutants exhibiting delayed dark-induced senescence |
| title_full | Global metabolic profiling of Arabidopsis Polyamine Oxidase 4 (AtPAO4) loss-of-function mutants exhibiting delayed dark-induced senescence |
| title_fullStr | Global metabolic profiling of Arabidopsis Polyamine Oxidase 4 (AtPAO4) loss-of-function mutants exhibiting delayed dark-induced senescence |
| title_full_unstemmed | Global metabolic profiling of Arabidopsis Polyamine Oxidase 4 (AtPAO4) loss-of-function mutants exhibiting delayed dark-induced senescence |
| title_short | Global metabolic profiling of Arabidopsis Polyamine Oxidase 4 (AtPAO4) loss-of-function mutants exhibiting delayed dark-induced senescence |
| title_sort | global metabolic profiling of arabidopsis polyamine oxidase 4 atpao4 loss of function mutants exhibiting delayed dark induced senescence |
| topic | Arabidopsis Oxidative Stress Polyamines Spermine senescence Polyamine oxidase (PAO) |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00173/full |
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