Screening and Identification of Novel Drug-resistant Genes in CD133+ and CD133- Lung Adenosarcoma Cells Using cDNA Microarray
Background and objective Cancer stem cells (CSCs) are responsible for multi-drug resistance in tumors. CD133 is a known biomarker of CSCs. The aim of this study is to screen for drug-resistant differentially expressed genes in CD133+ and CD133- lung cancer cells and to identify novel lung tumor drug...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | zho |
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Chinese Anti-Cancer Association; Chinese Antituberculosis Association
2014-06-01
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| Series: | Chinese Journal of Lung Cancer |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.3779/j.issn.1009-3419.2014.06.01 |
| _version_ | 1818954868502560768 |
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| author | Hongyan WANG Shaoqiu ZHENG Yongsheng TU Yajie ZHANG |
| author_facet | Hongyan WANG Shaoqiu ZHENG Yongsheng TU Yajie ZHANG |
| author_sort | Hongyan WANG |
| collection | DOAJ |
| description | Background and objective Cancer stem cells (CSCs) are responsible for multi-drug resistance in tumors. CD133 is a known biomarker of CSCs. The aim of this study is to screen for drug-resistant differentially expressed genes in CD133+ and CD133- lung cancer cells and to identify novel lung tumor drug-resistant genes. Methods Magnetic activated cell sorting was used to isolate CD133+ and CD133- cells from human lung cancer cell line A549, and drug-resistant microarray was used to detect drug-resistant genes in the these cells. RT-qPCR was used to examine the expression of six lung tumor drug-resistant genes in pre- and post-chemotherapeutic A549 cells. Results A total of 31 differentially expressed genes were screened by microarray analysis. Of these genes, 30 were upregulated and one was downregulated in CD133+ cells compared with CD133- cells. Results were verified by RT-qPCR. CYP2C19, CYP2D6, CYP2E1, GSK3α, PPARα, and PPARβ/δ were significantly upregulated after the A549 cells were treated with 1.97 μg/mL DDP or 0.61 μg/mL doxorubicin for 48 h. Conclusion The drug resistance of lung adenosarcoma may be correlated with 31 differentially expressed genes screened by drug-resistant microarray. CYP2C19, CYP2D6, CYP2E1, GSK3α, PPARα, and PPARβ/δ might be novel lung adenosarcoma drug-resistant genes. |
| first_indexed | 2024-12-20T10:29:00Z |
| format | Article |
| id | doaj.art-93cf2410afaf4b0eae964e763a6d0d68 |
| institution | Directory Open Access Journal |
| issn | 1009-3419 |
| language | zho |
| last_indexed | 2024-12-20T10:29:00Z |
| publishDate | 2014-06-01 |
| publisher | Chinese Anti-Cancer Association; Chinese Antituberculosis Association |
| record_format | Article |
| series | Chinese Journal of Lung Cancer |
| spelling | doaj.art-93cf2410afaf4b0eae964e763a6d0d682022-12-21T19:43:46ZzhoChinese Anti-Cancer Association; Chinese Antituberculosis AssociationChinese Journal of Lung Cancer1009-34192014-06-0117643744310.3779/j.issn.1009-3419.2014.06.01Screening and Identification of Novel Drug-resistant Genes in CD133+ and CD133- Lung Adenosarcoma Cells Using cDNA MicroarrayHongyan WANG0Shaoqiu ZHENG1Yongsheng TU2Yajie ZHANG3Department of Pathology, Guangzhou Medical University, Guangzhou 510182, ChinaDepartment of Pathology, Guangzhou Medical University, Guangzhou 510182, ChinaDepartment of Physiology, Guangzhou Medical University, Guangzhou 510182, ChinaDepartment of Pathology, Guangzhou Medical University, Guangzhou 510182, ChinaBackground and objective Cancer stem cells (CSCs) are responsible for multi-drug resistance in tumors. CD133 is a known biomarker of CSCs. The aim of this study is to screen for drug-resistant differentially expressed genes in CD133+ and CD133- lung cancer cells and to identify novel lung tumor drug-resistant genes. Methods Magnetic activated cell sorting was used to isolate CD133+ and CD133- cells from human lung cancer cell line A549, and drug-resistant microarray was used to detect drug-resistant genes in the these cells. RT-qPCR was used to examine the expression of six lung tumor drug-resistant genes in pre- and post-chemotherapeutic A549 cells. Results A total of 31 differentially expressed genes were screened by microarray analysis. Of these genes, 30 were upregulated and one was downregulated in CD133+ cells compared with CD133- cells. Results were verified by RT-qPCR. CYP2C19, CYP2D6, CYP2E1, GSK3α, PPARα, and PPARβ/δ were significantly upregulated after the A549 cells were treated with 1.97 μg/mL DDP or 0.61 μg/mL doxorubicin for 48 h. Conclusion The drug resistance of lung adenosarcoma may be correlated with 31 differentially expressed genes screened by drug-resistant microarray. CYP2C19, CYP2D6, CYP2E1, GSK3α, PPARα, and PPARβ/δ might be novel lung adenosarcoma drug-resistant genes.http://dx.doi.org/10.3779/j.issn.1009-3419.2014.06.01CD133Lung neoplasmsMulti-drug resistantDrug-resistant microarray |
| spellingShingle | Hongyan WANG Shaoqiu ZHENG Yongsheng TU Yajie ZHANG Screening and Identification of Novel Drug-resistant Genes in CD133+ and CD133- Lung Adenosarcoma Cells Using cDNA Microarray Chinese Journal of Lung Cancer CD133 Lung neoplasms Multi-drug resistant Drug-resistant microarray |
| title | Screening and Identification of Novel Drug-resistant Genes in CD133+ and CD133- Lung Adenosarcoma Cells Using cDNA Microarray |
| title_full | Screening and Identification of Novel Drug-resistant Genes in CD133+ and CD133- Lung Adenosarcoma Cells Using cDNA Microarray |
| title_fullStr | Screening and Identification of Novel Drug-resistant Genes in CD133+ and CD133- Lung Adenosarcoma Cells Using cDNA Microarray |
| title_full_unstemmed | Screening and Identification of Novel Drug-resistant Genes in CD133+ and CD133- Lung Adenosarcoma Cells Using cDNA Microarray |
| title_short | Screening and Identification of Novel Drug-resistant Genes in CD133+ and CD133- Lung Adenosarcoma Cells Using cDNA Microarray |
| title_sort | screening and identification of novel drug resistant genes in cd133 and cd133 lung adenosarcoma cells using cdna microarray |
| topic | CD133 Lung neoplasms Multi-drug resistant Drug-resistant microarray |
| url | http://dx.doi.org/10.3779/j.issn.1009-3419.2014.06.01 |
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