MiR-193a regulates chemoresistance of human osteosarcoma cells via repression of IRS2
Chemoresistance prevents curative potential of chemotherapy in most cases. MicroRNAs (miRNAs) are key players in regulating chemoresistance in osteosarcoma, which is the most common primary bone cancer. Bisulfite sequencing and quantitative real time PCR analyses showed that miR-193a expression is d...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2019-08-01
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| Series: | Journal of Bone Oncology |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2212137419300557 |
| _version_ | 1828181455098871808 |
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| author | Haiyan Wang Fangfang Zhao Shanbao Cai Youguang Pu |
| author_facet | Haiyan Wang Fangfang Zhao Shanbao Cai Youguang Pu |
| author_sort | Haiyan Wang |
| collection | DOAJ |
| description | Chemoresistance prevents curative potential of chemotherapy in most cases. MicroRNAs (miRNAs) are key players in regulating chemoresistance in osteosarcoma, which is the most common primary bone cancer. Bisulfite sequencing and quantitative real time PCR analyses showed that miR-193a expression is downregulated by DNA hypermethylation at its promoter region in a chemoresistant cell line, SJSA-1, compared to a chemosensitive cell line G-292. Introduction of a miR-193a mimic in SJSA-1 cells or an antagomir into G-292 cells confirmed the role of miR-193a in osteosarcoma chemoresistance. Bioinformatics together with biochemical assays showed that insulin receptor substrate 2 (IRS2) is a target of miR-193a. Our data concludes that miR-193a plays a role in the osteosarcoma chemoresistance and thus might serve as a useful biomarker for osteosarcoma prognosis. Keywords: Osteosarcoma, Chemoresistance, DNA methylation, miR-193a-3p, IRS2 |
| first_indexed | 2024-04-12T06:00:35Z |
| format | Article |
| id | doaj.art-93d398e6a30c4acbb7450a134e4f4fd2 |
| institution | Directory Open Access Journal |
| issn | 2212-1374 |
| language | English |
| last_indexed | 2024-04-12T06:00:35Z |
| publishDate | 2019-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Bone Oncology |
| spelling | doaj.art-93d398e6a30c4acbb7450a134e4f4fd22022-12-22T03:45:02ZengElsevierJournal of Bone Oncology2212-13742019-08-0117MiR-193a regulates chemoresistance of human osteosarcoma cells via repression of IRS2Haiyan Wang0Fangfang Zhao1Shanbao Cai2Youguang Pu3Department of Clinical Geriatrics, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230001, PR ChinaDepartment of Laboratory Research Center, Anhui Provincial Cancer Hospital, West Branch of the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230001, PR ChinaDepartment of Orthopedic Surgery, Anhui Provincial Cancer Hospital, West Branch of the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230001, PR ChinaDepartment of Laboratory Research Center, Anhui Provincial Cancer Hospital, West Branch of the First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230001, PR China; Corresponding author.Chemoresistance prevents curative potential of chemotherapy in most cases. MicroRNAs (miRNAs) are key players in regulating chemoresistance in osteosarcoma, which is the most common primary bone cancer. Bisulfite sequencing and quantitative real time PCR analyses showed that miR-193a expression is downregulated by DNA hypermethylation at its promoter region in a chemoresistant cell line, SJSA-1, compared to a chemosensitive cell line G-292. Introduction of a miR-193a mimic in SJSA-1 cells or an antagomir into G-292 cells confirmed the role of miR-193a in osteosarcoma chemoresistance. Bioinformatics together with biochemical assays showed that insulin receptor substrate 2 (IRS2) is a target of miR-193a. Our data concludes that miR-193a plays a role in the osteosarcoma chemoresistance and thus might serve as a useful biomarker for osteosarcoma prognosis. Keywords: Osteosarcoma, Chemoresistance, DNA methylation, miR-193a-3p, IRS2http://www.sciencedirect.com/science/article/pii/S2212137419300557 |
| spellingShingle | Haiyan Wang Fangfang Zhao Shanbao Cai Youguang Pu MiR-193a regulates chemoresistance of human osteosarcoma cells via repression of IRS2 Journal of Bone Oncology |
| title | MiR-193a regulates chemoresistance of human osteosarcoma cells via repression of IRS2 |
| title_full | MiR-193a regulates chemoresistance of human osteosarcoma cells via repression of IRS2 |
| title_fullStr | MiR-193a regulates chemoresistance of human osteosarcoma cells via repression of IRS2 |
| title_full_unstemmed | MiR-193a regulates chemoresistance of human osteosarcoma cells via repression of IRS2 |
| title_short | MiR-193a regulates chemoresistance of human osteosarcoma cells via repression of IRS2 |
| title_sort | mir 193a regulates chemoresistance of human osteosarcoma cells via repression of irs2 |
| url | http://www.sciencedirect.com/science/article/pii/S2212137419300557 |
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