RNA-seq analysis of antibacterial mechanism of Cinnamomum camphora essential oil against Escherichia coli
Background Transcriptome analysis plays a central role in elucidating the complexity of gene expression regulation in Escherichia coli. In recent years, the overuse of antibiotics has led to an increase in antimicrobial resistance, which greatly reduces the efficacy of antibacterial drugs and affect...
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PeerJ Inc.
2021-03-01
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| Online Access: | https://peerj.com/articles/11081.pdf |
| _version_ | 1797418329751683072 |
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| author | Yutian Yu Jie Dong Yanlu Wang Xi Gong |
| author_facet | Yutian Yu Jie Dong Yanlu Wang Xi Gong |
| author_sort | Yutian Yu |
| collection | DOAJ |
| description | Background Transcriptome analysis plays a central role in elucidating the complexity of gene expression regulation in Escherichia coli. In recent years, the overuse of antibiotics has led to an increase in antimicrobial resistance, which greatly reduces the efficacy of antibacterial drugs and affects people’s health. Therefore, several researchers are focused on finding other materials, which could replace or supplement antibiotic treatment. Methods E. coli was treated with water, acetone and Cinnamomum camphora essential oils, respectively. The antibacterial activity was assessed using the minimum inhibitory concentration (MIC), the minimum bactericidal concentration (MBC), the dry weight and the wet weight of the cells. To explore the antibacterial mechanism of the oil, the RNA-Seq analysis was adopted under three different treatments. Finally, the expression of related genes was verified by Quantitative PCR. Results In this study, we showed that the C. Camphora essential oil exerted a strong antibacterial effect. Our results showed that the inhibitory efficiency increased with increasing of the concentration of essential oil. RNA-seq analysis indicated that the essential oil inhibited the growth of E. coli by inhibiting the metabolism, chemotaxis, and adhesion, meanwhile, life activities were maintained by enhancing E. coli resistance reactions. These results are contributed to uncover the antimicrobial mechanisms of essential oils against E. coli, and the C. Camphora essential oil could be applied as an antibacterial agent to replace or ally with antibiotic. |
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| language | English |
| last_indexed | 2024-03-09T06:31:12Z |
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| spelling | doaj.art-93d4d40f681a407fbaa07164ad93235a2023-12-03T11:04:51ZengPeerJ Inc.PeerJ2167-83592021-03-019e1108110.7717/peerj.11081RNA-seq analysis of antibacterial mechanism of Cinnamomum camphora essential oil against Escherichia coliYutian Yu0Jie Dong1Yanlu Wang2Xi Gong3Human Aging Research Institute and School of Life Science and Jiangxi Key Laboratory of Human Aging, Nanchang University, Nanchang, Jiangxi, ChinaHuman Aging Research Institute and School of Life Science and Jiangxi Key Laboratory of Human Aging, Nanchang University, Nanchang, Jiangxi, ChinaHuman Aging Research Institute and School of Life Science and Jiangxi Key Laboratory of Human Aging, Nanchang University, Nanchang, Jiangxi, ChinaHuman Aging Research Institute and School of Life Science and Jiangxi Key Laboratory of Human Aging, Nanchang University, Nanchang, Jiangxi, ChinaBackground Transcriptome analysis plays a central role in elucidating the complexity of gene expression regulation in Escherichia coli. In recent years, the overuse of antibiotics has led to an increase in antimicrobial resistance, which greatly reduces the efficacy of antibacterial drugs and affects people’s health. Therefore, several researchers are focused on finding other materials, which could replace or supplement antibiotic treatment. Methods E. coli was treated with water, acetone and Cinnamomum camphora essential oils, respectively. The antibacterial activity was assessed using the minimum inhibitory concentration (MIC), the minimum bactericidal concentration (MBC), the dry weight and the wet weight of the cells. To explore the antibacterial mechanism of the oil, the RNA-Seq analysis was adopted under three different treatments. Finally, the expression of related genes was verified by Quantitative PCR. Results In this study, we showed that the C. Camphora essential oil exerted a strong antibacterial effect. Our results showed that the inhibitory efficiency increased with increasing of the concentration of essential oil. RNA-seq analysis indicated that the essential oil inhibited the growth of E. coli by inhibiting the metabolism, chemotaxis, and adhesion, meanwhile, life activities were maintained by enhancing E. coli resistance reactions. These results are contributed to uncover the antimicrobial mechanisms of essential oils against E. coli, and the C. Camphora essential oil could be applied as an antibacterial agent to replace or ally with antibiotic.https://peerj.com/articles/11081.pdfEscherichia coli Cinnamomum camphora essential oil Antibacterial mechanismRNA-seqGene expression regulation |
| spellingShingle | Yutian Yu Jie Dong Yanlu Wang Xi Gong RNA-seq analysis of antibacterial mechanism of Cinnamomum camphora essential oil against Escherichia coli PeerJ Escherichia coli Cinnamomum camphora essential oil Antibacterial mechanism RNA-seq Gene expression regulation |
| title | RNA-seq analysis of antibacterial mechanism of Cinnamomum camphora essential oil against Escherichia coli |
| title_full | RNA-seq analysis of antibacterial mechanism of Cinnamomum camphora essential oil against Escherichia coli |
| title_fullStr | RNA-seq analysis of antibacterial mechanism of Cinnamomum camphora essential oil against Escherichia coli |
| title_full_unstemmed | RNA-seq analysis of antibacterial mechanism of Cinnamomum camphora essential oil against Escherichia coli |
| title_short | RNA-seq analysis of antibacterial mechanism of Cinnamomum camphora essential oil against Escherichia coli |
| title_sort | rna seq analysis of antibacterial mechanism of cinnamomum camphora essential oil against escherichia coli |
| topic | Escherichia coli Cinnamomum camphora essential oil Antibacterial mechanism RNA-seq Gene expression regulation |
| url | https://peerj.com/articles/11081.pdf |
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