The pgm locus and pigmentation phenotype in Yersinia pestis
The pigmentation (pgm) locus is a large unstable area of the Yersinia pestis chromosome composed of a segment of iron acquisition (HPI) linked to a pigmentation segment. In this work we examined the mobility of HPI and the pigmentation segment in three Y. pestis isolates using successive subcultures...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Genética
2006-01-01
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| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100024 |
| _version_ | 1818859732974174208 |
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| author | Tereza Cristina Leal-Balbino Nilma Cintra Leal Mirna Gisele Medeiros do Nascimento Maria Betânia Melo de Oliveira Valdir de Queiroz Balbino Alzira Maria Paiva de Almeida |
| author_facet | Tereza Cristina Leal-Balbino Nilma Cintra Leal Mirna Gisele Medeiros do Nascimento Maria Betânia Melo de Oliveira Valdir de Queiroz Balbino Alzira Maria Paiva de Almeida |
| author_sort | Tereza Cristina Leal-Balbino |
| collection | DOAJ |
| description | The pigmentation (pgm) locus is a large unstable area of the Yersinia pestis chromosome composed of a segment of iron acquisition (HPI) linked to a pigmentation segment. In this work we examined the mobility of HPI and the pigmentation segment in three Y. pestis isolates using successive subcultures on Congo red agar (CRA) plates. Strain P. CE 882 was shown to be highly stable while strains P. Exu 340 and P. Peru 375 dissociated into several phenotypes, PCR analysis showing evidence of changes in the pgm locus of the derived cultures. Strains P. Exu 340 and P. Peru 375 produced previously unreported cultures positive for the pesticin/yersiniabactin outer membrane receptor (psn+) but negative for the iron-regulated protein (irp2-), suggesting the occurrence of rearrangements in this chromosomal region and either a sequential loss or the loss of separated segments. These results provide evidence that besides deletion en bloc, specific rearrangements are also involved in the deletion events for that locus. |
| first_indexed | 2024-12-19T09:16:52Z |
| format | Article |
| id | doaj.art-93d52461cab04157994a65bbb945207a |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-19T09:16:52Z |
| publishDate | 2006-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-93d52461cab04157994a65bbb945207a2022-12-21T20:28:06ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852006-01-0129112613110.1590/S1415-47572006000100024The pgm locus and pigmentation phenotype in Yersinia pestisTereza Cristina Leal-BalbinoNilma Cintra LealMirna Gisele Medeiros do NascimentoMaria Betânia Melo de OliveiraValdir de Queiroz BalbinoAlzira Maria Paiva de AlmeidaThe pigmentation (pgm) locus is a large unstable area of the Yersinia pestis chromosome composed of a segment of iron acquisition (HPI) linked to a pigmentation segment. In this work we examined the mobility of HPI and the pigmentation segment in three Y. pestis isolates using successive subcultures on Congo red agar (CRA) plates. Strain P. CE 882 was shown to be highly stable while strains P. Exu 340 and P. Peru 375 dissociated into several phenotypes, PCR analysis showing evidence of changes in the pgm locus of the derived cultures. Strains P. Exu 340 and P. Peru 375 produced previously unreported cultures positive for the pesticin/yersiniabactin outer membrane receptor (psn+) but negative for the iron-regulated protein (irp2-), suggesting the occurrence of rearrangements in this chromosomal region and either a sequential loss or the loss of separated segments. These results provide evidence that besides deletion en bloc, specific rearrangements are also involved in the deletion events for that locus.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100024Y. pestishigh pathogenicity islandpgmpsn |
| spellingShingle | Tereza Cristina Leal-Balbino Nilma Cintra Leal Mirna Gisele Medeiros do Nascimento Maria Betânia Melo de Oliveira Valdir de Queiroz Balbino Alzira Maria Paiva de Almeida The pgm locus and pigmentation phenotype in Yersinia pestis Genetics and Molecular Biology Y. pestis high pathogenicity island pgm psn |
| title | The pgm locus and pigmentation phenotype in Yersinia pestis |
| title_full | The pgm locus and pigmentation phenotype in Yersinia pestis |
| title_fullStr | The pgm locus and pigmentation phenotype in Yersinia pestis |
| title_full_unstemmed | The pgm locus and pigmentation phenotype in Yersinia pestis |
| title_short | The pgm locus and pigmentation phenotype in Yersinia pestis |
| title_sort | pgm locus and pigmentation phenotype in yersinia pestis |
| topic | Y. pestis high pathogenicity island pgm psn |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100024 |
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