Epidemiological survey in Łęczyńsko-Włodawskie Lake District of eastern Poland reveals new evidence of zoonotic potential of Giardia intestinalis
Faecal samples from 297 farm animals were collected from 18 households in distinct sites of the Łęczyńsko-Włodawskie Lake District of eastern Poland. They included samples from 86 cattle ( Bos taurus ), 84 pigs ( Sus scrofa f. domestica ), 81 sheep ( Ovis aries ), 10 horses ( Equus caballus ), and 3...
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Institute of Rural Health
2015-12-01
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| Series: | Annals of Agricultural and Environmental Medicine |
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| Online Access: | http://www.journalssystem.com/aaem/Epidemiological-survey-in-Leczynsko-Wlodawskie-Lake-District-of-eastern-Poland-reveals-new-evidence-of-zoonotic-potential-of-Giardia-intestinalis,72334,0,2.html |
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| author | Krzysztof Stojecki Jacek Sroka Tomasz Cencek Jacek Dutkiewicz |
| author_facet | Krzysztof Stojecki Jacek Sroka Tomasz Cencek Jacek Dutkiewicz |
| author_sort | Krzysztof Stojecki |
| collection | DOAJ |
| description | Faecal samples from 297 farm animals were collected from 18 households in distinct sites of the Łęczyńsko-Włodawskie Lake District of eastern Poland. They included samples from 86 cattle ( Bos taurus ), 84 pigs ( Sus scrofa f. domestica ), 81 sheep ( Ovis aries ), 10 horses ( Equus caballus ), and 36 dogs ( Canis lupus familiaris ). The samples were examined for the presence of Giardia intestinalis by the Direct Fluorescence Assay (DFA) and semi-nested PCR. All amplicons were sequenced on both strands. By DFA, cysts of Giardia spp. were detected in 66 of 297 faecal samples (22.2%). Positive specimens for Giardia spp. were derived from 29.8% of examined pigs, 21.0% of sheep, 18.6% of cattle, 10% of horses, and 19.4% of dogs. Based on the detection of the β-giardin gene by PCR, 39 (13.1%) of the 297 examined samples were recognized as positive. Detection of the presence of Giardia cysts by DFA test was overall significantly higher compared to PCR (p=0.0045). By PCR, Giardia was found in 28.1% of sheep, 11.6% of cattle, 10% of horses, 9.5% of pigs and 5.6% of dogs. Partial β-giardin gene sequences were obtained for 73.7% of the PCR positive samples. From sequenced samples derived from the studied animals, Giardia were identified as assemblage A (8 samples), B (1 sample) and E (18 samples). As assemblages A and B may be zoonotic, the farm animals living in eastern Poland could be regarded as a potential source of Giardia infection for humans. |
| first_indexed | 2024-04-12T18:38:05Z |
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| id | doaj.art-93d5f9422eb94f5eb1ee14d1526e915d |
| institution | Directory Open Access Journal |
| issn | 1232-1966 1898-2263 |
| language | English |
| last_indexed | 2024-04-12T18:38:05Z |
| publishDate | 2015-12-01 |
| publisher | Institute of Rural Health |
| record_format | Article |
| series | Annals of Agricultural and Environmental Medicine |
| spelling | doaj.art-93d5f9422eb94f5eb1ee14d1526e915d2022-12-22T03:20:52ZengInstitute of Rural HealthAnnals of Agricultural and Environmental Medicine1232-19661898-22632015-12-0122459459810.5604/12321966.118575972334Epidemiological survey in Łęczyńsko-Włodawskie Lake District of eastern Poland reveals new evidence of zoonotic potential of Giardia intestinalisKrzysztof Stojecki0Jacek Sroka1Tomasz Cencek2Jacek Dutkiewicz3Department of Parasitology, National Veterinary Research Institute, Pulawy, PolandDepartment of Parasitology, National Veterinary Research Institute, Pulawy, PolandDepartment of Parasitology, National Veterinary Research Institute, Pulawy, PolandDepartment of Health Biohazards and Parasitology, Institute of Rural Health, Lublin, PolandFaecal samples from 297 farm animals were collected from 18 households in distinct sites of the Łęczyńsko-Włodawskie Lake District of eastern Poland. They included samples from 86 cattle ( Bos taurus ), 84 pigs ( Sus scrofa f. domestica ), 81 sheep ( Ovis aries ), 10 horses ( Equus caballus ), and 36 dogs ( Canis lupus familiaris ). The samples were examined for the presence of Giardia intestinalis by the Direct Fluorescence Assay (DFA) and semi-nested PCR. All amplicons were sequenced on both strands. By DFA, cysts of Giardia spp. were detected in 66 of 297 faecal samples (22.2%). Positive specimens for Giardia spp. were derived from 29.8% of examined pigs, 21.0% of sheep, 18.6% of cattle, 10% of horses, and 19.4% of dogs. Based on the detection of the β-giardin gene by PCR, 39 (13.1%) of the 297 examined samples were recognized as positive. Detection of the presence of Giardia cysts by DFA test was overall significantly higher compared to PCR (p=0.0045). By PCR, Giardia was found in 28.1% of sheep, 11.6% of cattle, 10% of horses, 9.5% of pigs and 5.6% of dogs. Partial β-giardin gene sequences were obtained for 73.7% of the PCR positive samples. From sequenced samples derived from the studied animals, Giardia were identified as assemblage A (8 samples), B (1 sample) and E (18 samples). As assemblages A and B may be zoonotic, the farm animals living in eastern Poland could be regarded as a potential source of Giardia infection for humans.http://www.journalssystem.com/aaem/Epidemiological-survey-in-Leczynsko-Wlodawskie-Lake-District-of-eastern-Poland-reveals-new-evidence-of-zoonotic-potential-of-Giardia-intestinalis,72334,0,2.htmlGiardia intestinalisfarm animalsfaecal samplesEastern PolandDirect Fluorescence AssayPCR |
| spellingShingle | Krzysztof Stojecki Jacek Sroka Tomasz Cencek Jacek Dutkiewicz Epidemiological survey in Łęczyńsko-Włodawskie Lake District of eastern Poland reveals new evidence of zoonotic potential of Giardia intestinalis Annals of Agricultural and Environmental Medicine Giardia intestinalis farm animals faecal samples Eastern Poland Direct Fluorescence Assay PCR |
| title | Epidemiological survey in Łęczyńsko-Włodawskie Lake District of eastern Poland reveals new evidence of zoonotic potential of Giardia intestinalis |
| title_full | Epidemiological survey in Łęczyńsko-Włodawskie Lake District of eastern Poland reveals new evidence of zoonotic potential of Giardia intestinalis |
| title_fullStr | Epidemiological survey in Łęczyńsko-Włodawskie Lake District of eastern Poland reveals new evidence of zoonotic potential of Giardia intestinalis |
| title_full_unstemmed | Epidemiological survey in Łęczyńsko-Włodawskie Lake District of eastern Poland reveals new evidence of zoonotic potential of Giardia intestinalis |
| title_short | Epidemiological survey in Łęczyńsko-Włodawskie Lake District of eastern Poland reveals new evidence of zoonotic potential of Giardia intestinalis |
| title_sort | epidemiological survey in leczynsko wlodawskie lake district of eastern poland reveals new evidence of zoonotic potential of giardia intestinalis |
| topic | Giardia intestinalis farm animals faecal samples Eastern Poland Direct Fluorescence Assay PCR |
| url | http://www.journalssystem.com/aaem/Epidemiological-survey-in-Leczynsko-Wlodawskie-Lake-District-of-eastern-Poland-reveals-new-evidence-of-zoonotic-potential-of-Giardia-intestinalis,72334,0,2.html |
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