Grapevine rootstocks differentially affect the rate of ripening and modulate auxin-related genes in Cabernet Sauvignon berries
In modern viticulture, grafting commercial grapevine varieties on interspecific rootstocks is a common practice required for conferring resistance to many biotic and abiotic stresses. Nevertheless, the use of rootstocks to gain these essential traits is also known to impact grape berry development a...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-02-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00069/full |
| _version_ | 1811217158913392640 |
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| author | Massimiliano eCorso Massimiliano eCorso Alessandro eVannozzi Alessandro eVannozzi Fiorenza eZiliotto Mohamed eZouine Tommaso eNicolato Elie eMaza Nicola eVitulo Franco eMeggio Franco eMeggio Giorgio eValle Mondher eBouzayen Maren eMüller Sergi eMunné-Bosch Margherita eLucchin Margherita eLucchin Claudio eBonghi Claudio eBonghi |
| author_facet | Massimiliano eCorso Massimiliano eCorso Alessandro eVannozzi Alessandro eVannozzi Fiorenza eZiliotto Mohamed eZouine Tommaso eNicolato Elie eMaza Nicola eVitulo Franco eMeggio Franco eMeggio Giorgio eValle Mondher eBouzayen Maren eMüller Sergi eMunné-Bosch Margherita eLucchin Margherita eLucchin Claudio eBonghi Claudio eBonghi |
| author_sort | Massimiliano eCorso |
| collection | DOAJ |
| description | In modern viticulture, grafting commercial grapevine varieties on interspecific rootstocks is a common practice required for conferring resistance to many biotic and abiotic stresses. Nevertheless, the use of rootstocks to gain these essential traits is also known to impact grape berry development and quality, although the underlying mechanisms are still poorly understood. In grape berries, the onset of ripening (véraison) is regulated by a complex network of mobile signals including hormones such as auxins, ethylene, abscisic acid and brassinosteroids. Recently, a new rootstock, designated M4, was selected based on its enhanced tolerance to water stress and medium vigour. This study investigates the effect of M4 on Cabernet Sauvignon (CS) berry development in comparison to the commercial 1103P rootstock. Physical and biochemical parameters showed that the ripening rate of CS berries is faster when grafted onto M4. A multifactorial analysis performed on mRNA-Seq data obtained from skin and pulp of berries grown in both graft combinations revealed that genes controlling auxin action (ARF and Aux/IAA) represent one of main categories affected by the rootstock genotype. Considering that the level of auxin tightly regulates the transcription of these genes, we investigated the behaviour of the main gene families involved in auxin biosynthesis and conjugation. Molecular and biochemical analyses confirmed a link between the rate of berry development and the modulation of auxin metabolism. Moreover the data indicate that this phenomenon appears to be particularly pronounced in skin tissue in comparison to the flesh. |
| first_indexed | 2024-04-12T06:50:43Z |
| format | Article |
| id | doaj.art-93d65a7ddb234a26a32f375950f25413 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-12T06:50:43Z |
| publishDate | 2016-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-93d65a7ddb234a26a32f375950f254132022-12-22T03:43:22ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-02-01710.3389/fpls.2016.00069169974Grapevine rootstocks differentially affect the rate of ripening and modulate auxin-related genes in Cabernet Sauvignon berriesMassimiliano eCorso0Massimiliano eCorso1Alessandro eVannozzi2Alessandro eVannozzi3Fiorenza eZiliotto4Mohamed eZouine5Tommaso eNicolato6Elie eMaza7Nicola eVitulo8Franco eMeggio9Franco eMeggio10Giorgio eValle11Mondher eBouzayen12Maren eMüller13Sergi eMunné-Bosch14Margherita eLucchin15Margherita eLucchin16Claudio eBonghi17Claudio eBonghi18University of PadovaUniversity of PadovaUniversity of PadovaUniversity of PadovaUniversity of PadovaInstitut National Polytechnique de ToulouseUniversity of PadovaInstitut National Polytechnique de ToulouseUniversity of PadovaUniversity of PadovaUniversity of PadovaUniversity of PadovaInstitut National Polytechnique de ToulouseUniversitat de BarcelonaUniversitat de BarcelonaUniversity of PadovaUniversity of PadovaUniversity of PadovaUniversity of PadovaIn modern viticulture, grafting commercial grapevine varieties on interspecific rootstocks is a common practice required for conferring resistance to many biotic and abiotic stresses. Nevertheless, the use of rootstocks to gain these essential traits is also known to impact grape berry development and quality, although the underlying mechanisms are still poorly understood. In grape berries, the onset of ripening (véraison) is regulated by a complex network of mobile signals including hormones such as auxins, ethylene, abscisic acid and brassinosteroids. Recently, a new rootstock, designated M4, was selected based on its enhanced tolerance to water stress and medium vigour. This study investigates the effect of M4 on Cabernet Sauvignon (CS) berry development in comparison to the commercial 1103P rootstock. Physical and biochemical parameters showed that the ripening rate of CS berries is faster when grafted onto M4. A multifactorial analysis performed on mRNA-Seq data obtained from skin and pulp of berries grown in both graft combinations revealed that genes controlling auxin action (ARF and Aux/IAA) represent one of main categories affected by the rootstock genotype. Considering that the level of auxin tightly regulates the transcription of these genes, we investigated the behaviour of the main gene families involved in auxin biosynthesis and conjugation. Molecular and biochemical analyses confirmed a link between the rate of berry development and the modulation of auxin metabolism. Moreover the data indicate that this phenomenon appears to be particularly pronounced in skin tissue in comparison to the flesh.http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00069/fullFruit developmentgrapevineTranscriptional programAuxin conjugationpolyphenols biosynthesis |
| spellingShingle | Massimiliano eCorso Massimiliano eCorso Alessandro eVannozzi Alessandro eVannozzi Fiorenza eZiliotto Mohamed eZouine Tommaso eNicolato Elie eMaza Nicola eVitulo Franco eMeggio Franco eMeggio Giorgio eValle Mondher eBouzayen Maren eMüller Sergi eMunné-Bosch Margherita eLucchin Margherita eLucchin Claudio eBonghi Claudio eBonghi Grapevine rootstocks differentially affect the rate of ripening and modulate auxin-related genes in Cabernet Sauvignon berries Frontiers in Plant Science Fruit development grapevine Transcriptional program Auxin conjugation polyphenols biosynthesis |
| title | Grapevine rootstocks differentially affect the rate of ripening and modulate auxin-related genes in Cabernet Sauvignon berries |
| title_full | Grapevine rootstocks differentially affect the rate of ripening and modulate auxin-related genes in Cabernet Sauvignon berries |
| title_fullStr | Grapevine rootstocks differentially affect the rate of ripening and modulate auxin-related genes in Cabernet Sauvignon berries |
| title_full_unstemmed | Grapevine rootstocks differentially affect the rate of ripening and modulate auxin-related genes in Cabernet Sauvignon berries |
| title_short | Grapevine rootstocks differentially affect the rate of ripening and modulate auxin-related genes in Cabernet Sauvignon berries |
| title_sort | grapevine rootstocks differentially affect the rate of ripening and modulate auxin related genes in cabernet sauvignon berries |
| topic | Fruit development grapevine Transcriptional program Auxin conjugation polyphenols biosynthesis |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00069/full |
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