Comparative genomic analysis of human fungal pathogens causing paracoccidioidomycosis.
Paracoccidioides is a fungal pathogen and the cause of paracoccidioidomycosis, a health-threatening human systemic mycosis endemic to Latin America. Infection by Paracoccidioides, a dimorphic fungus in the order Onygenales, is coupled with a thermally regulated transition from a soil-dwelling filame...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-10-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC3203195?pdf=render |
| _version_ | 1817977680641916928 |
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| author | Christopher A Desjardins Mia D Champion Jason W Holder Anna Muszewska Jonathan Goldberg Alexandre M Bailão Marcelo Macedo Brigido Márcia Eliana da Silva Ferreira Ana Maria Garcia Marcin Grynberg Sharvari Gujja David I Heiman Matthew R Henn Chinnappa D Kodira Henry León-Narváez Larissa V G Longo Li-Jun Ma Iran Malavazi Alisson L Matsuo Flavia V Morais Maristela Pereira Sabrina Rodríguez-Brito Sharadha Sakthikumar Silvia M Salem-Izacc Sean M Sykes Marcus Melo Teixeira Milene C Vallejo Maria Emília Machado Telles Walter Chandri Yandava Sarah Young Qiandong Zeng Jeremy Zucker Maria Sueli Felipe Gustavo H Goldman Brian J Haas Juan G McEwen Gustavo Nino-Vega Rosana Puccia Gioconda San-Blas Celia Maria de Almeida Soares Bruce W Birren Christina A Cuomo |
| author_facet | Christopher A Desjardins Mia D Champion Jason W Holder Anna Muszewska Jonathan Goldberg Alexandre M Bailão Marcelo Macedo Brigido Márcia Eliana da Silva Ferreira Ana Maria Garcia Marcin Grynberg Sharvari Gujja David I Heiman Matthew R Henn Chinnappa D Kodira Henry León-Narváez Larissa V G Longo Li-Jun Ma Iran Malavazi Alisson L Matsuo Flavia V Morais Maristela Pereira Sabrina Rodríguez-Brito Sharadha Sakthikumar Silvia M Salem-Izacc Sean M Sykes Marcus Melo Teixeira Milene C Vallejo Maria Emília Machado Telles Walter Chandri Yandava Sarah Young Qiandong Zeng Jeremy Zucker Maria Sueli Felipe Gustavo H Goldman Brian J Haas Juan G McEwen Gustavo Nino-Vega Rosana Puccia Gioconda San-Blas Celia Maria de Almeida Soares Bruce W Birren Christina A Cuomo |
| author_sort | Christopher A Desjardins |
| collection | DOAJ |
| description | Paracoccidioides is a fungal pathogen and the cause of paracoccidioidomycosis, a health-threatening human systemic mycosis endemic to Latin America. Infection by Paracoccidioides, a dimorphic fungus in the order Onygenales, is coupled with a thermally regulated transition from a soil-dwelling filamentous form to a yeast-like pathogenic form. To better understand the genetic basis of growth and pathogenicity in Paracoccidioides, we sequenced the genomes of two strains of Paracoccidioides brasiliensis (Pb03 and Pb18) and one strain of Paracoccidioides lutzii (Pb01). These genomes range in size from 29.1 Mb to 32.9 Mb and encode 7,610 to 8,130 genes. To enable genetic studies, we mapped 94% of the P. brasiliensis Pb18 assembly onto five chromosomes. We characterized gene family content across Onygenales and related fungi, and within Paracoccidioides we found expansions of the fungal-specific kinase family FunK1. Additionally, the Onygenales have lost many genes involved in carbohydrate metabolism and fewer genes involved in protein metabolism, resulting in a higher ratio of proteases to carbohydrate active enzymes in the Onygenales than their relatives. To determine if gene content correlated with growth on different substrates, we screened the non-pathogenic onygenale Uncinocarpus reesii, which has orthologs for 91% of Paracoccidioides metabolic genes, for growth on 190 carbon sources. U. reesii showed growth on a limited range of carbohydrates, primarily basic plant sugars and cell wall components; this suggests that Onygenales, including dimorphic fungi, can degrade cellulosic plant material in the soil. In addition, U. reesii grew on gelatin and a wide range of dipeptides and amino acids, indicating a preference for proteinaceous growth substrates over carbohydrates, which may enable these fungi to also degrade animal biomass. These capabilities for degrading plant and animal substrates suggest a duality in lifestyle that could enable pathogenic species of Onygenales to transfer from soil to animal hosts. |
| first_indexed | 2024-04-13T22:19:23Z |
| format | Article |
| id | doaj.art-93d6f7f011e24b3f851b66f333b84a0a |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-04-13T22:19:23Z |
| publishDate | 2011-10-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-93d6f7f011e24b3f851b66f333b84a0a2022-12-22T02:27:20ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042011-10-01710e100234510.1371/journal.pgen.1002345Comparative genomic analysis of human fungal pathogens causing paracoccidioidomycosis.Christopher A DesjardinsMia D ChampionJason W HolderAnna MuszewskaJonathan GoldbergAlexandre M BailãoMarcelo Macedo BrigidoMárcia Eliana da Silva FerreiraAna Maria GarciaMarcin GrynbergSharvari GujjaDavid I HeimanMatthew R HennChinnappa D KodiraHenry León-NarváezLarissa V G LongoLi-Jun MaIran MalavaziAlisson L MatsuoFlavia V MoraisMaristela PereiraSabrina Rodríguez-BritoSharadha SakthikumarSilvia M Salem-IzaccSean M SykesMarcus Melo TeixeiraMilene C VallejoMaria Emília Machado Telles WalterChandri YandavaSarah YoungQiandong ZengJeremy ZuckerMaria Sueli FelipeGustavo H GoldmanBrian J HaasJuan G McEwenGustavo Nino-VegaRosana PucciaGioconda San-BlasCelia Maria de Almeida SoaresBruce W BirrenChristina A CuomoParacoccidioides is a fungal pathogen and the cause of paracoccidioidomycosis, a health-threatening human systemic mycosis endemic to Latin America. Infection by Paracoccidioides, a dimorphic fungus in the order Onygenales, is coupled with a thermally regulated transition from a soil-dwelling filamentous form to a yeast-like pathogenic form. To better understand the genetic basis of growth and pathogenicity in Paracoccidioides, we sequenced the genomes of two strains of Paracoccidioides brasiliensis (Pb03 and Pb18) and one strain of Paracoccidioides lutzii (Pb01). These genomes range in size from 29.1 Mb to 32.9 Mb and encode 7,610 to 8,130 genes. To enable genetic studies, we mapped 94% of the P. brasiliensis Pb18 assembly onto five chromosomes. We characterized gene family content across Onygenales and related fungi, and within Paracoccidioides we found expansions of the fungal-specific kinase family FunK1. Additionally, the Onygenales have lost many genes involved in carbohydrate metabolism and fewer genes involved in protein metabolism, resulting in a higher ratio of proteases to carbohydrate active enzymes in the Onygenales than their relatives. To determine if gene content correlated with growth on different substrates, we screened the non-pathogenic onygenale Uncinocarpus reesii, which has orthologs for 91% of Paracoccidioides metabolic genes, for growth on 190 carbon sources. U. reesii showed growth on a limited range of carbohydrates, primarily basic plant sugars and cell wall components; this suggests that Onygenales, including dimorphic fungi, can degrade cellulosic plant material in the soil. In addition, U. reesii grew on gelatin and a wide range of dipeptides and amino acids, indicating a preference for proteinaceous growth substrates over carbohydrates, which may enable these fungi to also degrade animal biomass. These capabilities for degrading plant and animal substrates suggest a duality in lifestyle that could enable pathogenic species of Onygenales to transfer from soil to animal hosts.http://europepmc.org/articles/PMC3203195?pdf=render |
| spellingShingle | Christopher A Desjardins Mia D Champion Jason W Holder Anna Muszewska Jonathan Goldberg Alexandre M Bailão Marcelo Macedo Brigido Márcia Eliana da Silva Ferreira Ana Maria Garcia Marcin Grynberg Sharvari Gujja David I Heiman Matthew R Henn Chinnappa D Kodira Henry León-Narváez Larissa V G Longo Li-Jun Ma Iran Malavazi Alisson L Matsuo Flavia V Morais Maristela Pereira Sabrina Rodríguez-Brito Sharadha Sakthikumar Silvia M Salem-Izacc Sean M Sykes Marcus Melo Teixeira Milene C Vallejo Maria Emília Machado Telles Walter Chandri Yandava Sarah Young Qiandong Zeng Jeremy Zucker Maria Sueli Felipe Gustavo H Goldman Brian J Haas Juan G McEwen Gustavo Nino-Vega Rosana Puccia Gioconda San-Blas Celia Maria de Almeida Soares Bruce W Birren Christina A Cuomo Comparative genomic analysis of human fungal pathogens causing paracoccidioidomycosis. PLoS Genetics |
| title | Comparative genomic analysis of human fungal pathogens causing paracoccidioidomycosis. |
| title_full | Comparative genomic analysis of human fungal pathogens causing paracoccidioidomycosis. |
| title_fullStr | Comparative genomic analysis of human fungal pathogens causing paracoccidioidomycosis. |
| title_full_unstemmed | Comparative genomic analysis of human fungal pathogens causing paracoccidioidomycosis. |
| title_short | Comparative genomic analysis of human fungal pathogens causing paracoccidioidomycosis. |
| title_sort | comparative genomic analysis of human fungal pathogens causing paracoccidioidomycosis |
| url | http://europepmc.org/articles/PMC3203195?pdf=render |
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