The renal and hepatic distribution of Bence Jones proteins depends on glycosylation: a scintigraphic study in rats
The aim of the present study was to evaluate renal and liver distribution of two monoclonal immunoglobulin light chains. The chains were purified individually from the urine of patients with multiple myeloma and characterized as lambda light chains with a molecular mass of 28 kDa. They were named BJ...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
1997-07-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700008&tlng=en |
| _version_ | 1798014433490894848 |
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| author | M.J.B.A. Prado A.L. Nicastri P.L.A. Costa T. Rockman I.L.S. Tersariol H.B. Nader R.T. Barros E.B.A. Prado |
| author_facet | M.J.B.A. Prado A.L. Nicastri P.L.A. Costa T. Rockman I.L.S. Tersariol H.B. Nader R.T. Barros E.B.A. Prado |
| author_sort | M.J.B.A. Prado |
| collection | DOAJ |
| description | The aim of the present study was to evaluate renal and liver distribution of two monoclonal immunoglobulin light chains. The chains were purified individually from the urine of patients with multiple myeloma and characterized as lambda light chains with a molecular mass of 28 kDa. They were named BJg (high amount of galactose residues exposed) and BJs (sialic acid residues exposed) on the basis of carbohydrate content. A scintigraphic study was performed on male Wistar rats weighing 250 g for 60 min after iv administration of 1 mg of each protein (7.4 MBq), as the intact proteins and also after carbohydrate oxidation. Images were obtained with a Siemens gamma camera with a high-resolution collimator and processed with a MicroDelta system. Hepatic and renal distribution were established and are reported as percent of injected dose. Liver uptake of BJg was significantly higher than liver uptake of BJs (94.3 vs 81.4%) (P<0.05). This contributed to its greater removal from the intravascular compartment, and consequently lower kidney accumulation of BJg in comparison to BJs (5.7 vs 18.6%) (P<0.05). After carbohydrate oxidation, there was a decrease in hepatic accumulation of both proteins and consequently a higher renal overload. The tissue distribution of periodate-treated BJg was similar to that of native BJs: 82.7 vs 81.4% in the liver and 17.3 vs 18.6% in the kidneys. These observations indicate the important role of sugar residues of Bence Jones proteins for their recognition by specific membrane receptors, which leads to differential tissue accumulation and possible toxicity |
| first_indexed | 2024-04-11T15:17:23Z |
| format | Article |
| id | doaj.art-93d74c4b79264334b2aa78d6465d155c |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-04-11T15:17:23Z |
| publishDate | 1997-07-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-93d74c4b79264334b2aa78d6465d155c2022-12-22T04:16:27ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X1997-07-01307865872The renal and hepatic distribution of Bence Jones proteins depends on glycosylation: a scintigraphic study in ratsM.J.B.A. PradoA.L. NicastriP.L.A. CostaT. RockmanI.L.S. TersariolH.B. NaderR.T. BarrosE.B.A. PradoThe aim of the present study was to evaluate renal and liver distribution of two monoclonal immunoglobulin light chains. The chains were purified individually from the urine of patients with multiple myeloma and characterized as lambda light chains with a molecular mass of 28 kDa. They were named BJg (high amount of galactose residues exposed) and BJs (sialic acid residues exposed) on the basis of carbohydrate content. A scintigraphic study was performed on male Wistar rats weighing 250 g for 60 min after iv administration of 1 mg of each protein (7.4 MBq), as the intact proteins and also after carbohydrate oxidation. Images were obtained with a Siemens gamma camera with a high-resolution collimator and processed with a MicroDelta system. Hepatic and renal distribution were established and are reported as percent of injected dose. Liver uptake of BJg was significantly higher than liver uptake of BJs (94.3 vs 81.4%) (P<0.05). This contributed to its greater removal from the intravascular compartment, and consequently lower kidney accumulation of BJg in comparison to BJs (5.7 vs 18.6%) (P<0.05). After carbohydrate oxidation, there was a decrease in hepatic accumulation of both proteins and consequently a higher renal overload. The tissue distribution of periodate-treated BJg was similar to that of native BJs: 82.7 vs 81.4% in the liver and 17.3 vs 18.6% in the kidneys. These observations indicate the important role of sugar residues of Bence Jones proteins for their recognition by specific membrane receptors, which leads to differential tissue accumulation and possible toxicityhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700008&tlng=enBence Jones proteinsscintigraphic studymultiple myelomanephrotoxicity |
| spellingShingle | M.J.B.A. Prado A.L. Nicastri P.L.A. Costa T. Rockman I.L.S. Tersariol H.B. Nader R.T. Barros E.B.A. Prado The renal and hepatic distribution of Bence Jones proteins depends on glycosylation: a scintigraphic study in rats Brazilian Journal of Medical and Biological Research Bence Jones proteins scintigraphic study multiple myeloma nephrotoxicity |
| title | The renal and hepatic distribution of Bence Jones proteins depends on glycosylation: a scintigraphic study in rats |
| title_full | The renal and hepatic distribution of Bence Jones proteins depends on glycosylation: a scintigraphic study in rats |
| title_fullStr | The renal and hepatic distribution of Bence Jones proteins depends on glycosylation: a scintigraphic study in rats |
| title_full_unstemmed | The renal and hepatic distribution of Bence Jones proteins depends on glycosylation: a scintigraphic study in rats |
| title_short | The renal and hepatic distribution of Bence Jones proteins depends on glycosylation: a scintigraphic study in rats |
| title_sort | renal and hepatic distribution of bence jones proteins depends on glycosylation a scintigraphic study in rats |
| topic | Bence Jones proteins scintigraphic study multiple myeloma nephrotoxicity |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700008&tlng=en |
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