Isolation and Purification of metalloprotease produced by Aeromonas hydrophila
Background: The bacteria Aeromonas hydrophila was isolated from clinical specimens responsible of causing diarrhea in children and adults. Objective:To purify metalloprotease enzyme produced by Aeromonas hydrophila. Patients and Methods: This study involved 150 stool samples collected from pa...
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| Format: | Article |
| Language: | English |
| Published: |
College of medicine/ University of Diyala
2018-08-01
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| Series: | Diyala Journal of Medicine |
| Subjects: | |
| Online Access: | http://djm.uodiyala.edu.iq/index.php/djm/article/view/84 |
| _version_ | 1797454707884556288 |
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| author | Muhannad Moeen Abdallah Tahreer Hadi Saleh |
| author_facet | Muhannad Moeen Abdallah Tahreer Hadi Saleh |
| author_sort | Muhannad Moeen Abdallah |
| collection | DOAJ |
| description |
Background: The bacteria Aeromonas hydrophila was isolated from clinical specimens responsible of causing diarrhea in children and adults.
Objective:To purify metalloprotease enzyme produced by Aeromonas hydrophila.
Patients and Methods: This study involved 150 stool samples collected from patients suffering from diarrhea.
Results: Eight isolate of Aeromonas hydrophila was detected and metalloprotease was purified with 11.06 U/ml of enzyme activity.
Conclusion: The bacteria have ability to produce metalloprotease and enzyme was fully purified in three steps of purification.
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| first_indexed | 2024-03-09T15:41:57Z |
| format | Article |
| id | doaj.art-93db20ce585d461a9908ac723141d7c7 |
| institution | Directory Open Access Journal |
| issn | 2219-9764 2617-8982 |
| language | English |
| last_indexed | 2024-03-09T15:41:57Z |
| publishDate | 2018-08-01 |
| publisher | College of medicine/ University of Diyala |
| record_format | Article |
| series | Diyala Journal of Medicine |
| spelling | doaj.art-93db20ce585d461a9908ac723141d7c72023-11-24T23:24:55ZengCollege of medicine/ University of DiyalaDiyala Journal of Medicine2219-97642617-89822018-08-01142Isolation and Purification of metalloprotease produced by Aeromonas hydrophilaMuhannad Moeen AbdallahTahreer Hadi Saleh Background: The bacteria Aeromonas hydrophila was isolated from clinical specimens responsible of causing diarrhea in children and adults. Objective:To purify metalloprotease enzyme produced by Aeromonas hydrophila. Patients and Methods: This study involved 150 stool samples collected from patients suffering from diarrhea. Results: Eight isolate of Aeromonas hydrophila was detected and metalloprotease was purified with 11.06 U/ml of enzyme activity. Conclusion: The bacteria have ability to produce metalloprotease and enzyme was fully purified in three steps of purification. http://djm.uodiyala.edu.iq/index.php/djm/article/view/84Aeromonas hydrophila, Metalloprotease |
| spellingShingle | Muhannad Moeen Abdallah Tahreer Hadi Saleh Isolation and Purification of metalloprotease produced by Aeromonas hydrophila Diyala Journal of Medicine Aeromonas hydrophila, Metalloprotease |
| title | Isolation and Purification of metalloprotease produced by Aeromonas hydrophila |
| title_full | Isolation and Purification of metalloprotease produced by Aeromonas hydrophila |
| title_fullStr | Isolation and Purification of metalloprotease produced by Aeromonas hydrophila |
| title_full_unstemmed | Isolation and Purification of metalloprotease produced by Aeromonas hydrophila |
| title_short | Isolation and Purification of metalloprotease produced by Aeromonas hydrophila |
| title_sort | isolation and purification of metalloprotease produced by aeromonas hydrophila |
| topic | Aeromonas hydrophila, Metalloprotease |
| url | http://djm.uodiyala.edu.iq/index.php/djm/article/view/84 |
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