Effect of Lipopolysaccharides (LPS) and Lipoteichoic Acid (LTA) on the Inflammatory Response in Rumen Epithelial Cells (REC) and the Impact of LPS on Claw Explants
Endotoxins play a crucial role in ruminant health due to their deleterious effects on animal health. The study aimed to evaluate whether LPS and LTA can induce an inflammatory response in rumen epithelial cells. For this purpose, epithelial cells isolated from rumen tissue (REC) were stimulated with...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/7/2058 |
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| author | Nicole Reisinger Dominik Wendner Nora Schauerhuber Elisabeth Mayer |
| author_facet | Nicole Reisinger Dominik Wendner Nora Schauerhuber Elisabeth Mayer |
| author_sort | Nicole Reisinger |
| collection | DOAJ |
| description | Endotoxins play a crucial role in ruminant health due to their deleterious effects on animal health. The study aimed to evaluate whether LPS and LTA can induce an inflammatory response in rumen epithelial cells. For this purpose, epithelial cells isolated from rumen tissue (REC) were stimulated with LPS and LTA for 1, 2, 4, and 24 h. Thereafter, the expression of selected genes of the LPS and LTA pathway and inflammatory response were evaluated. Furthermore, it was assessed whether LPS affects inflammatory response and structural integrity of claw explants. Therefore, claw explants were incubated with LPS for 4 h to assess the expression of selected genes and for 24 h to evaluate tissue integrity via separation force. LPS strongly affected the expression of genes related to inflammation (NFkB, TNF-α, IL1B, IL6, CXCL8, MMP9) in REC. LTA induced a delayed and weaker inflammatory response than LPS. In claw explants, LPS affected tissue integrity, as there was a concentration-dependent decrease of separation force. Incubation time had a strong effect on inflammatory genes in claw explants. Our data suggest that endotoxins can induce a local inflammatory response in the rumen epithelium. Furthermore, translocation of LPS might negatively impact claw health. |
| first_indexed | 2024-03-10T09:48:06Z |
| format | Article |
| id | doaj.art-93db46938b5c4d5a83753fbe1a6b83ec |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T09:48:06Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-93db46938b5c4d5a83753fbe1a6b83ec2023-11-22T03:01:03ZengMDPI AGAnimals2076-26152021-07-01117205810.3390/ani11072058Effect of Lipopolysaccharides (LPS) and Lipoteichoic Acid (LTA) on the Inflammatory Response in Rumen Epithelial Cells (REC) and the Impact of LPS on Claw ExplantsNicole Reisinger0Dominik Wendner1Nora Schauerhuber2Elisabeth Mayer3BIOMIN Research Center, 3430 Tulln, AustriaBIOMIN Research Center, 3430 Tulln, AustriaBIOMIN Research Center, 3430 Tulln, AustriaBIOMIN Research Center, 3430 Tulln, AustriaEndotoxins play a crucial role in ruminant health due to their deleterious effects on animal health. The study aimed to evaluate whether LPS and LTA can induce an inflammatory response in rumen epithelial cells. For this purpose, epithelial cells isolated from rumen tissue (REC) were stimulated with LPS and LTA for 1, 2, 4, and 24 h. Thereafter, the expression of selected genes of the LPS and LTA pathway and inflammatory response were evaluated. Furthermore, it was assessed whether LPS affects inflammatory response and structural integrity of claw explants. Therefore, claw explants were incubated with LPS for 4 h to assess the expression of selected genes and for 24 h to evaluate tissue integrity via separation force. LPS strongly affected the expression of genes related to inflammation (NFkB, TNF-α, IL1B, IL6, CXCL8, MMP9) in REC. LTA induced a delayed and weaker inflammatory response than LPS. In claw explants, LPS affected tissue integrity, as there was a concentration-dependent decrease of separation force. Incubation time had a strong effect on inflammatory genes in claw explants. Our data suggest that endotoxins can induce a local inflammatory response in the rumen epithelium. Furthermore, translocation of LPS might negatively impact claw health.https://www.mdpi.com/2076-2615/11/7/2058rumen epithelial cellsendotoxinslipopolysaccharideslipoteichoic acidclaw explantsinflammation |
| spellingShingle | Nicole Reisinger Dominik Wendner Nora Schauerhuber Elisabeth Mayer Effect of Lipopolysaccharides (LPS) and Lipoteichoic Acid (LTA) on the Inflammatory Response in Rumen Epithelial Cells (REC) and the Impact of LPS on Claw Explants Animals rumen epithelial cells endotoxins lipopolysaccharides lipoteichoic acid claw explants inflammation |
| title | Effect of Lipopolysaccharides (LPS) and Lipoteichoic Acid (LTA) on the Inflammatory Response in Rumen Epithelial Cells (REC) and the Impact of LPS on Claw Explants |
| title_full | Effect of Lipopolysaccharides (LPS) and Lipoteichoic Acid (LTA) on the Inflammatory Response in Rumen Epithelial Cells (REC) and the Impact of LPS on Claw Explants |
| title_fullStr | Effect of Lipopolysaccharides (LPS) and Lipoteichoic Acid (LTA) on the Inflammatory Response in Rumen Epithelial Cells (REC) and the Impact of LPS on Claw Explants |
| title_full_unstemmed | Effect of Lipopolysaccharides (LPS) and Lipoteichoic Acid (LTA) on the Inflammatory Response in Rumen Epithelial Cells (REC) and the Impact of LPS on Claw Explants |
| title_short | Effect of Lipopolysaccharides (LPS) and Lipoteichoic Acid (LTA) on the Inflammatory Response in Rumen Epithelial Cells (REC) and the Impact of LPS on Claw Explants |
| title_sort | effect of lipopolysaccharides lps and lipoteichoic acid lta on the inflammatory response in rumen epithelial cells rec and the impact of lps on claw explants |
| topic | rumen epithelial cells endotoxins lipopolysaccharides lipoteichoic acid claw explants inflammation |
| url | https://www.mdpi.com/2076-2615/11/7/2058 |
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