Standard method for detecting Bombyx mori nucleopolyhedrovirus disease-resistant silkworm varieties
Bombyx mori nucleopolyhedrovirus (BmNPV) disease is one of the most serious silkworm diseases, and it has caused great economic losses to the sericulture industry. So far, the disease has not been controlled effectively by therapeutic agents. Breeding resistant silkworm varieties breeding may be an...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Entomologia
2018-01-01
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| Series: | Revista Brasileira de Entomologia |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S008556261730064X |
| _version_ | 1819074070623289344 |
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| author | Qiong Yang Dong Xu Xing Qing Rong Li Yang Xiao Ming Qiang Ye |
| author_facet | Qiong Yang Dong Xu Xing Qing Rong Li Yang Xiao Ming Qiang Ye |
| author_sort | Qiong Yang |
| collection | DOAJ |
| description | Bombyx mori nucleopolyhedrovirus (BmNPV) disease is one of the most serious silkworm diseases, and it has caused great economic losses to the sericulture industry. So far, the disease has not been controlled effectively by therapeutic agents. Breeding resistant silkworm varieties breeding may be an effective way to improve resistance to BmNPV and reduce economic losses. A precise resistance-detection method will help to accelerate the breeding process. For this purpose, here we described the individual inoculation method (IIM). Details of the IIM include pathogen BmNPV preparation, mulberry leaf size, pathogen volume, rearing conditions, course of infection, and breeding conditions. Finally, a resistance comparison experiment was performed using the IIM and the traditional group inoculation method (GIM). The incidence of BmNPV infection and the within-group variance results showed that the IIM was more precise and reliable than the GIM. Keywords: Bombyx mori nucleopolyhedrovirus, Group inoculation method, Individual inoculation method, Resistance detection, Silkworm |
| first_indexed | 2024-12-21T18:03:40Z |
| format | Article |
| id | doaj.art-93e23938559849bd9ca37b0d1748a2ed |
| institution | Directory Open Access Journal |
| issn | 0085-5626 |
| language | English |
| last_indexed | 2024-12-21T18:03:40Z |
| publishDate | 2018-01-01 |
| publisher | Sociedade Brasileira de Entomologia |
| record_format | Article |
| series | Revista Brasileira de Entomologia |
| spelling | doaj.art-93e23938559849bd9ca37b0d1748a2ed2022-12-21T18:55:00ZengSociedade Brasileira de EntomologiaRevista Brasileira de Entomologia0085-56262018-01-016211922Standard method for detecting Bombyx mori nucleopolyhedrovirus disease-resistant silkworm varietiesQiong Yang0Dong Xu Xing1Qing Rong Li2Yang Xiao3Ming Qiang Ye4Corresponding author.; Sericulture & Agri-food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, ChinaSericulture & Agri-food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, ChinaSericulture & Agri-food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, ChinaSericulture & Agri-food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, ChinaSericulture & Agri-food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, ChinaBombyx mori nucleopolyhedrovirus (BmNPV) disease is one of the most serious silkworm diseases, and it has caused great economic losses to the sericulture industry. So far, the disease has not been controlled effectively by therapeutic agents. Breeding resistant silkworm varieties breeding may be an effective way to improve resistance to BmNPV and reduce economic losses. A precise resistance-detection method will help to accelerate the breeding process. For this purpose, here we described the individual inoculation method (IIM). Details of the IIM include pathogen BmNPV preparation, mulberry leaf size, pathogen volume, rearing conditions, course of infection, and breeding conditions. Finally, a resistance comparison experiment was performed using the IIM and the traditional group inoculation method (GIM). The incidence of BmNPV infection and the within-group variance results showed that the IIM was more precise and reliable than the GIM. Keywords: Bombyx mori nucleopolyhedrovirus, Group inoculation method, Individual inoculation method, Resistance detection, Silkwormhttp://www.sciencedirect.com/science/article/pii/S008556261730064X |
| spellingShingle | Qiong Yang Dong Xu Xing Qing Rong Li Yang Xiao Ming Qiang Ye Standard method for detecting Bombyx mori nucleopolyhedrovirus disease-resistant silkworm varieties Revista Brasileira de Entomologia |
| title | Standard method for detecting Bombyx mori nucleopolyhedrovirus disease-resistant silkworm varieties |
| title_full | Standard method for detecting Bombyx mori nucleopolyhedrovirus disease-resistant silkworm varieties |
| title_fullStr | Standard method for detecting Bombyx mori nucleopolyhedrovirus disease-resistant silkworm varieties |
| title_full_unstemmed | Standard method for detecting Bombyx mori nucleopolyhedrovirus disease-resistant silkworm varieties |
| title_short | Standard method for detecting Bombyx mori nucleopolyhedrovirus disease-resistant silkworm varieties |
| title_sort | standard method for detecting bombyx mori nucleopolyhedrovirus disease resistant silkworm varieties |
| url | http://www.sciencedirect.com/science/article/pii/S008556261730064X |
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