Grass carp reovirus VP56 and VP35 induce formation of viral inclusion bodies for replication
Summary: Viral inclusion bodies (VIBs) are subcellular structures required for efficient viral replication. How type II grass carp reovirus (GCRV-II), the mainly prevalent strain, forms VIBs is unknown. In this study, we found that GCRV-II infection induced punctate VIBs in grass carp ovary (GCO) ce...
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Format: | Article |
Language: | English |
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Elsevier
2024-01-01
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Series: | iScience |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S258900422302761X |
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author | Chu Zhang Hui Wu Hao Feng Yong-An Zhang Jiagang Tu |
author_facet | Chu Zhang Hui Wu Hao Feng Yong-An Zhang Jiagang Tu |
author_sort | Chu Zhang |
collection | DOAJ |
description | Summary: Viral inclusion bodies (VIBs) are subcellular structures required for efficient viral replication. How type II grass carp reovirus (GCRV-II), the mainly prevalent strain, forms VIBs is unknown. In this study, we found that GCRV-II infection induced punctate VIBs in grass carp ovary (GCO) cells and that non-structural protein 38 (NS38) functioned as a participant in VIB formation. Furthermore, VP56 and VP35 induced VIBs and recruited other viral proteins via the N-terminal of VP56 and the middle domain of VP35. Additionally, we found that the newly synthesized viral RNAs co-localized with VP56 and VP35 in VIBs during infection. Taken together, VP56 and VP35 induce VIB formation and recruit other viral proteins and viral RNAs to the VIBs for viral replication, which helps identify new targets for developing anti-GCRV-II drugs to disrupt viral replication. |
first_indexed | 2024-03-08T22:14:24Z |
format | Article |
id | doaj.art-93e353dfccaf4797b972b61bb7351a58 |
institution | Directory Open Access Journal |
issn | 2589-0042 |
language | English |
last_indexed | 2024-03-08T22:14:24Z |
publishDate | 2024-01-01 |
publisher | Elsevier |
record_format | Article |
series | iScience |
spelling | doaj.art-93e353dfccaf4797b972b61bb7351a582023-12-19T04:17:19ZengElsevieriScience2589-00422024-01-01271108684Grass carp reovirus VP56 and VP35 induce formation of viral inclusion bodies for replicationChu Zhang0Hui Wu1Hao Feng2Yong-An Zhang3Jiagang Tu4State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, College of Fisheries, Huazhong Agricultural University, Wuhan, ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, ChinaState Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, ChinaState Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, College of Fisheries, Huazhong Agricultural University, Wuhan, China; Corresponding authorState Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, College of Fisheries, Huazhong Agricultural University, Wuhan, China; Corresponding authorSummary: Viral inclusion bodies (VIBs) are subcellular structures required for efficient viral replication. How type II grass carp reovirus (GCRV-II), the mainly prevalent strain, forms VIBs is unknown. In this study, we found that GCRV-II infection induced punctate VIBs in grass carp ovary (GCO) cells and that non-structural protein 38 (NS38) functioned as a participant in VIB formation. Furthermore, VP56 and VP35 induced VIBs and recruited other viral proteins via the N-terminal of VP56 and the middle domain of VP35. Additionally, we found that the newly synthesized viral RNAs co-localized with VP56 and VP35 in VIBs during infection. Taken together, VP56 and VP35 induce VIB formation and recruit other viral proteins and viral RNAs to the VIBs for viral replication, which helps identify new targets for developing anti-GCRV-II drugs to disrupt viral replication.http://www.sciencedirect.com/science/article/pii/S258900422302761XVirologyFish culture |
spellingShingle | Chu Zhang Hui Wu Hao Feng Yong-An Zhang Jiagang Tu Grass carp reovirus VP56 and VP35 induce formation of viral inclusion bodies for replication iScience Virology Fish culture |
title | Grass carp reovirus VP56 and VP35 induce formation of viral inclusion bodies for replication |
title_full | Grass carp reovirus VP56 and VP35 induce formation of viral inclusion bodies for replication |
title_fullStr | Grass carp reovirus VP56 and VP35 induce formation of viral inclusion bodies for replication |
title_full_unstemmed | Grass carp reovirus VP56 and VP35 induce formation of viral inclusion bodies for replication |
title_short | Grass carp reovirus VP56 and VP35 induce formation of viral inclusion bodies for replication |
title_sort | grass carp reovirus vp56 and vp35 induce formation of viral inclusion bodies for replication |
topic | Virology Fish culture |
url | http://www.sciencedirect.com/science/article/pii/S258900422302761X |
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