Mycobacterial Populations Partly Change the Proportions of the Cells Undergoing Asymmetric/Symmetric Divisions in Response to Glycerol Levels in Growth Medium
Twenty to thirty percent of the septating mycobacterial cells of the mid-log phase population showed highly deviated asymmetric constriction during division (ACD), while the remaining underwent symmetric constriction during division (SCD). The ACD produced short-sized cells (SCs) and normal/long-siz...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-05-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/10/5/1160 |
| _version_ | 1797534678258810880 |
|---|---|
| author | Atul Pradhan Nagaraja Mukkayyan Kishor Jakkala Parthasarathi Ajitkumar |
| author_facet | Atul Pradhan Nagaraja Mukkayyan Kishor Jakkala Parthasarathi Ajitkumar |
| author_sort | Atul Pradhan |
| collection | DOAJ |
| description | Twenty to thirty percent of the septating mycobacterial cells of the mid-log phase population showed highly deviated asymmetric constriction during division (ACD), while the remaining underwent symmetric constriction during division (SCD). The ACD produced short-sized cells (SCs) and normal/long-sized cells (NCs) as the sister–daughter cells, but with significant differential susceptibility to antibiotic/oxidative/nitrite stress. Here we report that, at 0.2% glycerol, formulated in the Middlebrook 7H9 medium, a significantly high proportion of the cells were divided by SCD. When the glycerol concentration decreased to 0.1% due to cell-growth/division, the ACD proportion gradually increased until the ACD:SCD ratio reached ~50:50. With further decrease in the glycerol levels, the SCD proportion increased with concomitant decrease in the ACD proportion. Maintenance of glycerol at 0.1%, through replenishment, held the ACD:SCD proportion at ~50:50. Transfer of the cells from one culture with a specific glycerol level to the supernatant from another culture, with a different glycerol level, made the cells change the ACD:SCD proportion to that of the culture from which the supernatant was taken. RT-qPCR data showed the possibility of diadenosine tetraphosphate phosphorylase (<i>MSMEG_2932</i>), phosphatidylinositol synthase (<i>MSMEG_2933</i>), and a Nudix family hydrolase (<i>MSMEG_2936</i>) involved in the ACD:SCD proportion-change in response to glycerol levels. We also discussed its physiological significance. |
| first_indexed | 2024-03-10T11:32:52Z |
| format | Article |
| id | doaj.art-93e7369618af4e589c1f8cb7ae97bc41 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T11:32:52Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-93e7369618af4e589c1f8cb7ae97bc412023-11-21T19:06:29ZengMDPI AGCells2073-44092021-05-01105116010.3390/cells10051160Mycobacterial Populations Partly Change the Proportions of the Cells Undergoing Asymmetric/Symmetric Divisions in Response to Glycerol Levels in Growth MediumAtul Pradhan0Nagaraja Mukkayyan1Kishor Jakkala2Parthasarathi Ajitkumar3Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, Karnataka, IndiaDepartment of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, Karnataka, IndiaDepartment of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, Karnataka, IndiaDepartment of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560012, Karnataka, IndiaTwenty to thirty percent of the septating mycobacterial cells of the mid-log phase population showed highly deviated asymmetric constriction during division (ACD), while the remaining underwent symmetric constriction during division (SCD). The ACD produced short-sized cells (SCs) and normal/long-sized cells (NCs) as the sister–daughter cells, but with significant differential susceptibility to antibiotic/oxidative/nitrite stress. Here we report that, at 0.2% glycerol, formulated in the Middlebrook 7H9 medium, a significantly high proportion of the cells were divided by SCD. When the glycerol concentration decreased to 0.1% due to cell-growth/division, the ACD proportion gradually increased until the ACD:SCD ratio reached ~50:50. With further decrease in the glycerol levels, the SCD proportion increased with concomitant decrease in the ACD proportion. Maintenance of glycerol at 0.1%, through replenishment, held the ACD:SCD proportion at ~50:50. Transfer of the cells from one culture with a specific glycerol level to the supernatant from another culture, with a different glycerol level, made the cells change the ACD:SCD proportion to that of the culture from which the supernatant was taken. RT-qPCR data showed the possibility of diadenosine tetraphosphate phosphorylase (<i>MSMEG_2932</i>), phosphatidylinositol synthase (<i>MSMEG_2933</i>), and a Nudix family hydrolase (<i>MSMEG_2936</i>) involved in the ACD:SCD proportion-change in response to glycerol levels. We also discussed its physiological significance.https://www.mdpi.com/2073-4409/10/5/1160asymmetric constriction during divisionsymmetric constriction during divisionasymmetric/symmetric division switchingglycerol levels<i>Mycobacterium smegmatis</i>gene knockout mutants |
| spellingShingle | Atul Pradhan Nagaraja Mukkayyan Kishor Jakkala Parthasarathi Ajitkumar Mycobacterial Populations Partly Change the Proportions of the Cells Undergoing Asymmetric/Symmetric Divisions in Response to Glycerol Levels in Growth Medium Cells asymmetric constriction during division symmetric constriction during division asymmetric/symmetric division switching glycerol levels <i>Mycobacterium smegmatis</i> gene knockout mutants |
| title | Mycobacterial Populations Partly Change the Proportions of the Cells Undergoing Asymmetric/Symmetric Divisions in Response to Glycerol Levels in Growth Medium |
| title_full | Mycobacterial Populations Partly Change the Proportions of the Cells Undergoing Asymmetric/Symmetric Divisions in Response to Glycerol Levels in Growth Medium |
| title_fullStr | Mycobacterial Populations Partly Change the Proportions of the Cells Undergoing Asymmetric/Symmetric Divisions in Response to Glycerol Levels in Growth Medium |
| title_full_unstemmed | Mycobacterial Populations Partly Change the Proportions of the Cells Undergoing Asymmetric/Symmetric Divisions in Response to Glycerol Levels in Growth Medium |
| title_short | Mycobacterial Populations Partly Change the Proportions of the Cells Undergoing Asymmetric/Symmetric Divisions in Response to Glycerol Levels in Growth Medium |
| title_sort | mycobacterial populations partly change the proportions of the cells undergoing asymmetric symmetric divisions in response to glycerol levels in growth medium |
| topic | asymmetric constriction during division symmetric constriction during division asymmetric/symmetric division switching glycerol levels <i>Mycobacterium smegmatis</i> gene knockout mutants |
| url | https://www.mdpi.com/2073-4409/10/5/1160 |
| work_keys_str_mv | AT atulpradhan mycobacterialpopulationspartlychangetheproportionsofthecellsundergoingasymmetricsymmetricdivisionsinresponsetoglycerollevelsingrowthmedium AT nagarajamukkayyan mycobacterialpopulationspartlychangetheproportionsofthecellsundergoingasymmetricsymmetricdivisionsinresponsetoglycerollevelsingrowthmedium AT kishorjakkala mycobacterialpopulationspartlychangetheproportionsofthecellsundergoingasymmetricsymmetricdivisionsinresponsetoglycerollevelsingrowthmedium AT parthasarathiajitkumar mycobacterialpopulationspartlychangetheproportionsofthecellsundergoingasymmetricsymmetricdivisionsinresponsetoglycerollevelsingrowthmedium |