Fecal Microbial Composition and Predicted Functional Profile in Irritable Bowel Syndrome Differ between Subtypes and Geographical Locations
Increasing evidence suggests a microbial pathogenesis in irritable bowel syndrome (IBS) but the relationship remains elusive. Fecal DNA samples from 120 patients with IBS, 82 Mexican (IBS-C: n = 33, IBS-D: n = 24, IBS-M: n = 25) and 38 British (IBS-C: n = 6, IBS-D: n = 27, IBS-M: n = 5), were availa...
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| Format: | Article |
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MDPI AG
2023-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/10/2493 |
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| author | Jose F. Garcia-Mazcorro Mercedes Amieva-Balmori Arturo Triana-Romero Bridgette Wilson Leanne Smith Job Reyes-Huerta Megan Rossi Kevin Whelan Jose M. Remes-Troche |
| author_facet | Jose F. Garcia-Mazcorro Mercedes Amieva-Balmori Arturo Triana-Romero Bridgette Wilson Leanne Smith Job Reyes-Huerta Megan Rossi Kevin Whelan Jose M. Remes-Troche |
| author_sort | Jose F. Garcia-Mazcorro |
| collection | DOAJ |
| description | Increasing evidence suggests a microbial pathogenesis in irritable bowel syndrome (IBS) but the relationship remains elusive. Fecal DNA samples from 120 patients with IBS, 82 Mexican (IBS-C: n = 33, IBS-D: n = 24, IBS-M: n = 25) and 38 British (IBS-C: n = 6, IBS-D: n = 27, IBS-M: n = 5), were available for analysis using 16S rRNA gene sequencing. Firmicutes (mean: 82.1%), Actinobacteria (10.2%), and Bacteroidetes (4.4%) were the most abundant taxa. The analysis of all samples (n = 120), and females (n = 94) only, showed no significant differences in bacterial microbiota, but the analysis of Mexican patients (n = 82) showed several differences in key taxa (e.g., <i>Faecalibacterium</i>) among the different IBS subtypes. In IBS-D there were significantly higher Bacteroidetes in British patients (n = 27) than in Mexican patients (n = 24), suggesting unique fecal microbiota signatures within the same IBS subtype. These differences in IBS-D were also observed at lower phylogenetic levels (e.g., higher Enterobacteriaceae and <i>Streptococcus</i> in Mexican patients) and were accompanied by differences in several alpha diversity metrics. Beta diversity was not different among IBS subtypes when using all samples, but the analysis of IBS-D patients revealed consistent differences between Mexican and British patients. This study suggests that fecal microbiota is different between IBS subtypes and also within each subtype depending on geographical location. |
| first_indexed | 2024-03-10T21:01:46Z |
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| id | doaj.art-93e968f1059c4e988a93a221548b5d30 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T21:01:46Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-93e968f1059c4e988a93a221548b5d302023-11-19T17:27:36ZengMDPI AGMicroorganisms2076-26072023-10-011110249310.3390/microorganisms11102493Fecal Microbial Composition and Predicted Functional Profile in Irritable Bowel Syndrome Differ between Subtypes and Geographical LocationsJose F. Garcia-Mazcorro0Mercedes Amieva-Balmori1Arturo Triana-Romero2Bridgette Wilson3Leanne Smith4Job Reyes-Huerta5Megan Rossi6Kevin Whelan7Jose M. Remes-Troche8Research and Development, MNA de Mexico, San Nicolas de los Garza 66477, MexicoInstituto de Investigaciones Médico Biológicas, Universidad Veracruzana, Veracruz 91700, MexicoInstituto de Investigaciones Médico Biológicas, Universidad Veracruzana, Veracruz 91700, MexicoDepartment of Nutritional Sciences, King’s College London, London WC2R 2LS, UKDepartment of Nutritional Sciences, King’s College London, London WC2R 2LS, UKInstituto de Investigaciones Médico Biológicas, Universidad Veracruzana, Veracruz 91700, MexicoDepartment of Nutritional Sciences, King’s College London, London WC2R 2LS, UKDepartment of Nutritional Sciences, King’s College London, London WC2R 2LS, UKInstituto de Investigaciones Médico Biológicas, Universidad Veracruzana, Veracruz 91700, MexicoIncreasing evidence suggests a microbial pathogenesis in irritable bowel syndrome (IBS) but the relationship remains elusive. Fecal DNA samples from 120 patients with IBS, 82 Mexican (IBS-C: n = 33, IBS-D: n = 24, IBS-M: n = 25) and 38 British (IBS-C: n = 6, IBS-D: n = 27, IBS-M: n = 5), were available for analysis using 16S rRNA gene sequencing. Firmicutes (mean: 82.1%), Actinobacteria (10.2%), and Bacteroidetes (4.4%) were the most abundant taxa. The analysis of all samples (n = 120), and females (n = 94) only, showed no significant differences in bacterial microbiota, but the analysis of Mexican patients (n = 82) showed several differences in key taxa (e.g., <i>Faecalibacterium</i>) among the different IBS subtypes. In IBS-D there were significantly higher Bacteroidetes in British patients (n = 27) than in Mexican patients (n = 24), suggesting unique fecal microbiota signatures within the same IBS subtype. These differences in IBS-D were also observed at lower phylogenetic levels (e.g., higher Enterobacteriaceae and <i>Streptococcus</i> in Mexican patients) and were accompanied by differences in several alpha diversity metrics. Beta diversity was not different among IBS subtypes when using all samples, but the analysis of IBS-D patients revealed consistent differences between Mexican and British patients. This study suggests that fecal microbiota is different between IBS subtypes and also within each subtype depending on geographical location.https://www.mdpi.com/2076-2607/11/10/2493irritable bowel syndromemicrobiomegut microbiota |
| spellingShingle | Jose F. Garcia-Mazcorro Mercedes Amieva-Balmori Arturo Triana-Romero Bridgette Wilson Leanne Smith Job Reyes-Huerta Megan Rossi Kevin Whelan Jose M. Remes-Troche Fecal Microbial Composition and Predicted Functional Profile in Irritable Bowel Syndrome Differ between Subtypes and Geographical Locations Microorganisms irritable bowel syndrome microbiome gut microbiota |
| title | Fecal Microbial Composition and Predicted Functional Profile in Irritable Bowel Syndrome Differ between Subtypes and Geographical Locations |
| title_full | Fecal Microbial Composition and Predicted Functional Profile in Irritable Bowel Syndrome Differ between Subtypes and Geographical Locations |
| title_fullStr | Fecal Microbial Composition and Predicted Functional Profile in Irritable Bowel Syndrome Differ between Subtypes and Geographical Locations |
| title_full_unstemmed | Fecal Microbial Composition and Predicted Functional Profile in Irritable Bowel Syndrome Differ between Subtypes and Geographical Locations |
| title_short | Fecal Microbial Composition and Predicted Functional Profile in Irritable Bowel Syndrome Differ between Subtypes and Geographical Locations |
| title_sort | fecal microbial composition and predicted functional profile in irritable bowel syndrome differ between subtypes and geographical locations |
| topic | irritable bowel syndrome microbiome gut microbiota |
| url | https://www.mdpi.com/2076-2607/11/10/2493 |
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