MiR-23b Promotes Porcine Preadipocyte Differentiation via <i>SESN3</i> and <i>ACSL4</i>
Genetic improvement of pork quality is one of the hot topics in pig germplasm innovation. Backfat thickness and intramuscular fat content are important indexes of meat quality. MiRNAs are becoming recognized as a crucial regulator of adipose development. Therefore, it is crucial to understand how mi...
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MDPI AG
2022-07-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/11/15/2339 |
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| author | Meng Li Na Zhang Jiao Li Wanfeng Zhang Wei Hei Mengting Ji Yang Yang Guoqing Cao Xiaohong Guo Bugao Li |
| author_facet | Meng Li Na Zhang Jiao Li Wanfeng Zhang Wei Hei Mengting Ji Yang Yang Guoqing Cao Xiaohong Guo Bugao Li |
| author_sort | Meng Li |
| collection | DOAJ |
| description | Genetic improvement of pork quality is one of the hot topics in pig germplasm innovation. Backfat thickness and intramuscular fat content are important indexes of meat quality. MiRNAs are becoming recognized as a crucial regulator of adipose development. Therefore, it is crucial to understand how miR-23b regulates fat metabolism at the molecular level. In the present study, Oil Red O staining, and Western blot were used to evaluate the effect of miR-23b on the differentiation of porcine preadipocytes. Dual-luciferase reporter gene assay, pulldown, and RIP were used to reveal the mechanism of miR-23b regulating cell differentiation. The findings demonstrated that miR-23b promotes the expression of adipogenic factors and increases the content of lipid droplets, thus promoting the differentiation of preadipocytes. Further research found that miR-23b can directly bind to the 3’UTR of <i>SESN3</i> to regulate adipogenic differentiation. In addition, it was speculated that miR-23b controls cell differentiation by positively regulating the expression of <i>ACSL4</i> in other ways. Here, we demonstrate that miR-23b promotes the differentiation of porcine preadipocytes by targeting <i>SESN3</i> and promoting the expression of <i>ACSL4</i>. The present study is meaningful to the improvement of pork quality and the development of animal husbandry. |
| first_indexed | 2024-03-09T10:09:37Z |
| format | Article |
| id | doaj.art-93eafa2c1b76481b837ce75f77ebc245 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T10:09:37Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-93eafa2c1b76481b837ce75f77ebc2452023-12-01T22:52:45ZengMDPI AGCells2073-44092022-07-011115233910.3390/cells11152339MiR-23b Promotes Porcine Preadipocyte Differentiation via <i>SESN3</i> and <i>ACSL4</i>Meng Li0Na Zhang1Jiao Li2Wanfeng Zhang3Wei Hei4Mengting Ji5Yang Yang6Guoqing Cao7Xiaohong Guo8Bugao Li9College of Animal Science, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Animal Science, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Animal Science, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Animal Science, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Animal Science, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Animal Science, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Animal Science, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Animal Science, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Animal Science, Shanxi Agricultural University, Taigu 030801, ChinaCollege of Animal Science, Shanxi Agricultural University, Taigu 030801, ChinaGenetic improvement of pork quality is one of the hot topics in pig germplasm innovation. Backfat thickness and intramuscular fat content are important indexes of meat quality. MiRNAs are becoming recognized as a crucial regulator of adipose development. Therefore, it is crucial to understand how miR-23b regulates fat metabolism at the molecular level. In the present study, Oil Red O staining, and Western blot were used to evaluate the effect of miR-23b on the differentiation of porcine preadipocytes. Dual-luciferase reporter gene assay, pulldown, and RIP were used to reveal the mechanism of miR-23b regulating cell differentiation. The findings demonstrated that miR-23b promotes the expression of adipogenic factors and increases the content of lipid droplets, thus promoting the differentiation of preadipocytes. Further research found that miR-23b can directly bind to the 3’UTR of <i>SESN3</i> to regulate adipogenic differentiation. In addition, it was speculated that miR-23b controls cell differentiation by positively regulating the expression of <i>ACSL4</i> in other ways. Here, we demonstrate that miR-23b promotes the differentiation of porcine preadipocytes by targeting <i>SESN3</i> and promoting the expression of <i>ACSL4</i>. The present study is meaningful to the improvement of pork quality and the development of animal husbandry.https://www.mdpi.com/2073-4409/11/15/2339pigmiR-23b<i>SESN3</i><i>ACSL4</i>preadipocyteadipogenic differentiation |
| spellingShingle | Meng Li Na Zhang Jiao Li Wanfeng Zhang Wei Hei Mengting Ji Yang Yang Guoqing Cao Xiaohong Guo Bugao Li MiR-23b Promotes Porcine Preadipocyte Differentiation via <i>SESN3</i> and <i>ACSL4</i> Cells pig miR-23b <i>SESN3</i> <i>ACSL4</i> preadipocyte adipogenic differentiation |
| title | MiR-23b Promotes Porcine Preadipocyte Differentiation via <i>SESN3</i> and <i>ACSL4</i> |
| title_full | MiR-23b Promotes Porcine Preadipocyte Differentiation via <i>SESN3</i> and <i>ACSL4</i> |
| title_fullStr | MiR-23b Promotes Porcine Preadipocyte Differentiation via <i>SESN3</i> and <i>ACSL4</i> |
| title_full_unstemmed | MiR-23b Promotes Porcine Preadipocyte Differentiation via <i>SESN3</i> and <i>ACSL4</i> |
| title_short | MiR-23b Promotes Porcine Preadipocyte Differentiation via <i>SESN3</i> and <i>ACSL4</i> |
| title_sort | mir 23b promotes porcine preadipocyte differentiation via i sesn3 i and i acsl4 i |
| topic | pig miR-23b <i>SESN3</i> <i>ACSL4</i> preadipocyte adipogenic differentiation |
| url | https://www.mdpi.com/2073-4409/11/15/2339 |
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