Weed Suppressing Potential and Isolation of Potent Plant Growth Inhibitors from Castanea crenata Sieb. et Zucc
This study isolated, determined, and quantified plant growth inhibitors in Japanese chestnut (Castanea crenata Sieb. et Zucc), a deciduous species native to Japan and Korea. In laboratory assays, C. crenata leaves showed strong inhibition on germination and seedling growth of Echinochloa crus-galli...
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| Format: | Article |
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MDPI AG
2018-02-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/23/2/345 |
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| author | Phung Thi Tuyen Tran Dang Xuan Truong Thi Tu Anh Truong Mai Van Ateeque Ahmad Abdelnaser Abdelghany Elzaawely Tran Dang Khanh |
| author_facet | Phung Thi Tuyen Tran Dang Xuan Truong Thi Tu Anh Truong Mai Van Ateeque Ahmad Abdelnaser Abdelghany Elzaawely Tran Dang Khanh |
| author_sort | Phung Thi Tuyen |
| collection | DOAJ |
| description | This study isolated, determined, and quantified plant growth inhibitors in Japanese chestnut (Castanea crenata Sieb. et Zucc), a deciduous species native to Japan and Korea. In laboratory assays, C. crenata leaves showed strong inhibition on germination and seedling growth of Echinochloa crus-galli (barnyardgrass), Lactuca sativa (lettuce), and Raphanus sativus (radish). Laboratory and greenhouse trials showed that leaves of C. crenata appeared as a promising material to manage weeds, especially the dicot weeds. By GC-MS and HPLC analyses, gallic, protocatechuic, p-hydroxybenzoic, caffeic, ferulic, ellagic, and cinnamic acids were identified and quantified, of which ellagic acid was present in the highest quantity (2.36 mg/g dried leaves). By column chromatography and spectral data (1H- and 13C-NMR, IR, and LC-MS) analysis, a compound identified as 2α,3β,7β,23-tetrahydroxyurs-12-ene-28-oic acid (1) was purified from the methanolic leaf extract of C. crenata (0.93 mg/g dried leaves). This constituent showed potent inhibition on growth of E. crus-galli, a problematic weed in agricultural practice. The inhibition of the compound 1 (IC50 = 2.62 and 0.41 mM) was >5 fold greater than that of p-hydroxybenzoic acid (IC50 = 15.33 and 2.11 mM) on shoot and root growth of E. crus-galli, respectively. Results suggest that the isolated the compound 1 has potential to develop natural herbicides to manage E. crus-galli. This study is the first to isolate and identify 2α,3β,7β,23-tetrahydroxyurs-12-ene-28-oic acid in a plant and report its plant growth inhibitory potential. |
| first_indexed | 2024-12-10T19:23:22Z |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-10T19:23:22Z |
| publishDate | 2018-02-01 |
| publisher | MDPI AG |
| record_format | Article |
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| spelling | doaj.art-93ec5dcd00ec4b239efe6b657854f3242022-12-22T01:36:26ZengMDPI AGMolecules1420-30492018-02-0123234510.3390/molecules23020345molecules23020345Weed Suppressing Potential and Isolation of Potent Plant Growth Inhibitors from Castanea crenata Sieb. et ZuccPhung Thi Tuyen0Tran Dang Xuan1Truong Thi Tu Anh2Truong Mai Van3Ateeque Ahmad4Abdelnaser Abdelghany Elzaawely5Tran Dang Khanh6Faculty of Forest Resources and Environmental Management, Vietnam National University of Forestry, Xuan Mai, Hanoi 156200, VietnamGraduate School for International Development and Cooperation, Hiroshima University, Higashi Hiroshima, Hiroshima 739-829, JapanGraduate School for International Development and Cooperation, Hiroshima University, Higashi Hiroshima, Hiroshima 739-829, JapanGraduate School for International Development and Cooperation, Hiroshima University, Higashi Hiroshima, Hiroshima 739-829, JapanChemical Engineering, CSIR, CIMAP, Kukrail Picnic Spot Road, Lucknow 226015, IndiaDepartment of Agricultural Botany, Faculty of Agriculture, Tanta University, Tanta 31527, EgyptDepartment of Genetic Engineering, Agricultural Genetics Institute, Pham Van Dong Street, Hanoi 122300, VietnamThis study isolated, determined, and quantified plant growth inhibitors in Japanese chestnut (Castanea crenata Sieb. et Zucc), a deciduous species native to Japan and Korea. In laboratory assays, C. crenata leaves showed strong inhibition on germination and seedling growth of Echinochloa crus-galli (barnyardgrass), Lactuca sativa (lettuce), and Raphanus sativus (radish). Laboratory and greenhouse trials showed that leaves of C. crenata appeared as a promising material to manage weeds, especially the dicot weeds. By GC-MS and HPLC analyses, gallic, protocatechuic, p-hydroxybenzoic, caffeic, ferulic, ellagic, and cinnamic acids were identified and quantified, of which ellagic acid was present in the highest quantity (2.36 mg/g dried leaves). By column chromatography and spectral data (1H- and 13C-NMR, IR, and LC-MS) analysis, a compound identified as 2α,3β,7β,23-tetrahydroxyurs-12-ene-28-oic acid (1) was purified from the methanolic leaf extract of C. crenata (0.93 mg/g dried leaves). This constituent showed potent inhibition on growth of E. crus-galli, a problematic weed in agricultural practice. The inhibition of the compound 1 (IC50 = 2.62 and 0.41 mM) was >5 fold greater than that of p-hydroxybenzoic acid (IC50 = 15.33 and 2.11 mM) on shoot and root growth of E. crus-galli, respectively. Results suggest that the isolated the compound 1 has potential to develop natural herbicides to manage E. crus-galli. This study is the first to isolate and identify 2α,3β,7β,23-tetrahydroxyurs-12-ene-28-oic acid in a plant and report its plant growth inhibitory potential.http://www.mdpi.com/1420-3049/23/2/345Castanea crenataallelochemicals2α,3β,7β,23-tetrahydroxyurs-12-ene-28-oic acidinhibitory potentialE. crus-gallimonocot weedsdicot weeds |
| spellingShingle | Phung Thi Tuyen Tran Dang Xuan Truong Thi Tu Anh Truong Mai Van Ateeque Ahmad Abdelnaser Abdelghany Elzaawely Tran Dang Khanh Weed Suppressing Potential and Isolation of Potent Plant Growth Inhibitors from Castanea crenata Sieb. et Zucc Molecules Castanea crenata allelochemicals 2α,3β,7β,23-tetrahydroxyurs-12-ene-28-oic acid inhibitory potential E. crus-galli monocot weeds dicot weeds |
| title | Weed Suppressing Potential and Isolation of Potent Plant Growth Inhibitors from Castanea crenata Sieb. et Zucc |
| title_full | Weed Suppressing Potential and Isolation of Potent Plant Growth Inhibitors from Castanea crenata Sieb. et Zucc |
| title_fullStr | Weed Suppressing Potential and Isolation of Potent Plant Growth Inhibitors from Castanea crenata Sieb. et Zucc |
| title_full_unstemmed | Weed Suppressing Potential and Isolation of Potent Plant Growth Inhibitors from Castanea crenata Sieb. et Zucc |
| title_short | Weed Suppressing Potential and Isolation of Potent Plant Growth Inhibitors from Castanea crenata Sieb. et Zucc |
| title_sort | weed suppressing potential and isolation of potent plant growth inhibitors from castanea crenata sieb et zucc |
| topic | Castanea crenata allelochemicals 2α,3β,7β,23-tetrahydroxyurs-12-ene-28-oic acid inhibitory potential E. crus-galli monocot weeds dicot weeds |
| url | http://www.mdpi.com/1420-3049/23/2/345 |
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