A Proteomic Study for the Discovery of Beef Tenderness Biomarkers and Prediction of Warner–Bratzler Shear Force Measured on <i>Longissimus thoracis</i> Muscles of Young Limousin-Sired Bulls
Beef tenderness is of central importance in determining consumers’ overall liking. To better understand the underlying mechanisms of tenderness and be able to predict it, this study aimed to apply a proteomics approach on the <i>Longissimus thoracis</i> (LT) muscle of young Limousin-sire...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-04-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/10/5/952 |
| _version_ | 1797536191700008960 |
|---|---|
| author | Yao Zhu Mohammed Gagaoua Anne Maria Mullen Alan L. Kelly Torres Sweeney Jamie Cafferky Didier Viala Ruth M. Hamill |
| author_facet | Yao Zhu Mohammed Gagaoua Anne Maria Mullen Alan L. Kelly Torres Sweeney Jamie Cafferky Didier Viala Ruth M. Hamill |
| author_sort | Yao Zhu |
| collection | DOAJ |
| description | Beef tenderness is of central importance in determining consumers’ overall liking. To better understand the underlying mechanisms of tenderness and be able to predict it, this study aimed to apply a proteomics approach on the <i>Longissimus thoracis</i> (LT) muscle of young Limousin-sired bulls to identify candidate protein biomarkers. A total of 34 proteins showed differential abundance between the tender and tough groups. These proteins belong to biological pathways related to muscle structure, energy metabolism, heat shock proteins, response to oxidative stress, and apoptosis. Twenty-three putative protein biomarkers or their isoforms had previously been identified as beef tenderness biomarkers, while eleven were novel. Using regression analysis to predict shear force values, MYOZ3 (Myozenin 3), BIN1 (Bridging Integrator-1), and OGN (Mimecan) were the major proteins retained in the regression model, together explaining 79% of the variability. The results of this study confirmed the existing knowledge but also offered new insights enriching the previous biomarkers of tenderness proposed for <i>Longissimus</i> muscle. |
| first_indexed | 2024-03-10T11:56:08Z |
| format | Article |
| id | doaj.art-93ed74b37f6446c98d3b30db020051dc |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T11:56:08Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-93ed74b37f6446c98d3b30db020051dc2023-11-21T17:18:58ZengMDPI AGFoods2304-81582021-04-0110595210.3390/foods10050952A Proteomic Study for the Discovery of Beef Tenderness Biomarkers and Prediction of Warner–Bratzler Shear Force Measured on <i>Longissimus thoracis</i> Muscles of Young Limousin-Sired BullsYao Zhu0Mohammed Gagaoua1Anne Maria Mullen2Alan L. Kelly3Torres Sweeney4Jamie Cafferky5Didier Viala6Ruth M. Hamill7Food Quality and Sensory Science Department, Teagasc Food Research Centre, Ashtown, D15 KN3K Dublin 15, IrelandFood Quality and Sensory Science Department, Teagasc Food Research Centre, Ashtown, D15 KN3K Dublin 15, IrelandFood Quality and Sensory Science Department, Teagasc Food Research Centre, Ashtown, D15 KN3K Dublin 15, IrelandSchool of Food and Nutritional Sciences, University College Cork, T12 K8AF Cork, IrelandSchool of Veterinary Sciences, University College Dublin, D04 V1W8 Dublin 4, IrelandFood Quality and Sensory Science Department, Teagasc Food Research Centre, Ashtown, D15 KN3K Dublin 15, IrelandMetabolomic and Proteomic Exploration Facility (PFEM), INRAE, F-63122 Saint-Genès-Champanelle, FranceFood Quality and Sensory Science Department, Teagasc Food Research Centre, Ashtown, D15 KN3K Dublin 15, IrelandBeef tenderness is of central importance in determining consumers’ overall liking. To better understand the underlying mechanisms of tenderness and be able to predict it, this study aimed to apply a proteomics approach on the <i>Longissimus thoracis</i> (LT) muscle of young Limousin-sired bulls to identify candidate protein biomarkers. A total of 34 proteins showed differential abundance between the tender and tough groups. These proteins belong to biological pathways related to muscle structure, energy metabolism, heat shock proteins, response to oxidative stress, and apoptosis. Twenty-three putative protein biomarkers or their isoforms had previously been identified as beef tenderness biomarkers, while eleven were novel. Using regression analysis to predict shear force values, MYOZ3 (Myozenin 3), BIN1 (Bridging Integrator-1), and OGN (Mimecan) were the major proteins retained in the regression model, together explaining 79% of the variability. The results of this study confirmed the existing knowledge but also offered new insights enriching the previous biomarkers of tenderness proposed for <i>Longissimus</i> muscle.https://www.mdpi.com/2304-8158/10/5/952foodomicsbeef tendernessbovine biomarkersmuscleproteomeliquid chromatography-tandem mass spectrometry (LC-MS/MS) |
| spellingShingle | Yao Zhu Mohammed Gagaoua Anne Maria Mullen Alan L. Kelly Torres Sweeney Jamie Cafferky Didier Viala Ruth M. Hamill A Proteomic Study for the Discovery of Beef Tenderness Biomarkers and Prediction of Warner–Bratzler Shear Force Measured on <i>Longissimus thoracis</i> Muscles of Young Limousin-Sired Bulls Foods foodomics beef tenderness bovine biomarkers muscle proteome liquid chromatography-tandem mass spectrometry (LC-MS/MS) |
| title | A Proteomic Study for the Discovery of Beef Tenderness Biomarkers and Prediction of Warner–Bratzler Shear Force Measured on <i>Longissimus thoracis</i> Muscles of Young Limousin-Sired Bulls |
| title_full | A Proteomic Study for the Discovery of Beef Tenderness Biomarkers and Prediction of Warner–Bratzler Shear Force Measured on <i>Longissimus thoracis</i> Muscles of Young Limousin-Sired Bulls |
| title_fullStr | A Proteomic Study for the Discovery of Beef Tenderness Biomarkers and Prediction of Warner–Bratzler Shear Force Measured on <i>Longissimus thoracis</i> Muscles of Young Limousin-Sired Bulls |
| title_full_unstemmed | A Proteomic Study for the Discovery of Beef Tenderness Biomarkers and Prediction of Warner–Bratzler Shear Force Measured on <i>Longissimus thoracis</i> Muscles of Young Limousin-Sired Bulls |
| title_short | A Proteomic Study for the Discovery of Beef Tenderness Biomarkers and Prediction of Warner–Bratzler Shear Force Measured on <i>Longissimus thoracis</i> Muscles of Young Limousin-Sired Bulls |
| title_sort | proteomic study for the discovery of beef tenderness biomarkers and prediction of warner bratzler shear force measured on i longissimus thoracis i muscles of young limousin sired bulls |
| topic | foodomics beef tenderness bovine biomarkers muscle proteome liquid chromatography-tandem mass spectrometry (LC-MS/MS) |
| url | https://www.mdpi.com/2304-8158/10/5/952 |
| work_keys_str_mv | AT yaozhu aproteomicstudyforthediscoveryofbeeftendernessbiomarkersandpredictionofwarnerbratzlershearforcemeasuredonilongissimusthoracisimusclesofyounglimousinsiredbulls AT mohammedgagaoua aproteomicstudyforthediscoveryofbeeftendernessbiomarkersandpredictionofwarnerbratzlershearforcemeasuredonilongissimusthoracisimusclesofyounglimousinsiredbulls AT annemariamullen aproteomicstudyforthediscoveryofbeeftendernessbiomarkersandpredictionofwarnerbratzlershearforcemeasuredonilongissimusthoracisimusclesofyounglimousinsiredbulls AT alanlkelly aproteomicstudyforthediscoveryofbeeftendernessbiomarkersandpredictionofwarnerbratzlershearforcemeasuredonilongissimusthoracisimusclesofyounglimousinsiredbulls AT torressweeney aproteomicstudyforthediscoveryofbeeftendernessbiomarkersandpredictionofwarnerbratzlershearforcemeasuredonilongissimusthoracisimusclesofyounglimousinsiredbulls AT jamiecafferky aproteomicstudyforthediscoveryofbeeftendernessbiomarkersandpredictionofwarnerbratzlershearforcemeasuredonilongissimusthoracisimusclesofyounglimousinsiredbulls AT didierviala aproteomicstudyforthediscoveryofbeeftendernessbiomarkersandpredictionofwarnerbratzlershearforcemeasuredonilongissimusthoracisimusclesofyounglimousinsiredbulls AT ruthmhamill aproteomicstudyforthediscoveryofbeeftendernessbiomarkersandpredictionofwarnerbratzlershearforcemeasuredonilongissimusthoracisimusclesofyounglimousinsiredbulls AT yaozhu proteomicstudyforthediscoveryofbeeftendernessbiomarkersandpredictionofwarnerbratzlershearforcemeasuredonilongissimusthoracisimusclesofyounglimousinsiredbulls AT mohammedgagaoua proteomicstudyforthediscoveryofbeeftendernessbiomarkersandpredictionofwarnerbratzlershearforcemeasuredonilongissimusthoracisimusclesofyounglimousinsiredbulls AT annemariamullen proteomicstudyforthediscoveryofbeeftendernessbiomarkersandpredictionofwarnerbratzlershearforcemeasuredonilongissimusthoracisimusclesofyounglimousinsiredbulls AT alanlkelly proteomicstudyforthediscoveryofbeeftendernessbiomarkersandpredictionofwarnerbratzlershearforcemeasuredonilongissimusthoracisimusclesofyounglimousinsiredbulls AT torressweeney proteomicstudyforthediscoveryofbeeftendernessbiomarkersandpredictionofwarnerbratzlershearforcemeasuredonilongissimusthoracisimusclesofyounglimousinsiredbulls AT jamiecafferky proteomicstudyforthediscoveryofbeeftendernessbiomarkersandpredictionofwarnerbratzlershearforcemeasuredonilongissimusthoracisimusclesofyounglimousinsiredbulls AT didierviala proteomicstudyforthediscoveryofbeeftendernessbiomarkersandpredictionofwarnerbratzlershearforcemeasuredonilongissimusthoracisimusclesofyounglimousinsiredbulls AT ruthmhamill proteomicstudyforthediscoveryofbeeftendernessbiomarkersandpredictionofwarnerbratzlershearforcemeasuredonilongissimusthoracisimusclesofyounglimousinsiredbulls |