Analysis of Long Noncoding RNA and mRNA Expression Profiles in IL-9-Activated Astrocytes and EAE Mice
Background/Aims: Multiple sclerosis (MS) is an autoimmune disease in the central nervous system associated with demyelination and axonal injury. Astrocyte activation is involved in the pathogenesis of MS and experimental autoimmune encephalomyelitis (EAE), an animal model of MS. This study was desig...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Cell Physiol Biochem Press GmbH & Co KG
2018-03-01
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| Series: | Cellular Physiology and Biochemistry |
| Subjects: | |
| Online Access: | https://www.karger.com/Article/FullText/487975 |
| _version_ | 1818508754082070528 |
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| author | Xiaomei Liu Qing Zhang Weixiao Wang Dongjiao Zuo Jing Wang Feng Zhou Liping Niu Xiangyang Li Suping Qin Yanbo Kou Fanyun Kong Wei Pan Yugang Wang Dianshuai Gao Hong Sun Jessica M. Meves Kuiyang Zheng Renxian Tang |
| author_facet | Xiaomei Liu Qing Zhang Weixiao Wang Dongjiao Zuo Jing Wang Feng Zhou Liping Niu Xiangyang Li Suping Qin Yanbo Kou Fanyun Kong Wei Pan Yugang Wang Dianshuai Gao Hong Sun Jessica M. Meves Kuiyang Zheng Renxian Tang |
| author_sort | Xiaomei Liu |
| collection | DOAJ |
| description | Background/Aims: Multiple sclerosis (MS) is an autoimmune disease in the central nervous system associated with demyelination and axonal injury. Astrocyte activation is involved in the pathogenesis of MS and experimental autoimmune encephalomyelitis (EAE), an animal model of MS. This study was designed to find potential lncRNAs in EAE mice and activated astrocytes. Methods: we performed microarray analysis of lncRNAs from the brain tissues of EAE mice and primary mouse astrocytes treated with IL-9(50 ng/ml). 12 lncRNAs were validated through real-time PCR. Gene ontology and KEGG pathway analysis were applied to explore the potential functions of lncRNAs. Results: Differentially expressed 3300 lncRNAs and 3250 mRNAs were in the brain tissues of EAE mice, and 3748 lncRNAs and 3332 mRNAs were in activated astrocytes. Notably, there were 2 co-up-regulated lncRNAs and 3 co-down-regulated lncRNAs both in the brain tissues of EAE mice and in activated astrocytes, including Gm14005, Gm12478, mouselincRNA1117, AK080435, and mouselincRNA0681, which regulate the ER calcium flux kinetics, zinc finger protein and cell apoptosis. Similarly, there were 7 mRNAs co-up-regulated and 2 mRNAs co-down-regulated both in vivo and in vitro. Gene ontology and KEGG pathway analysis showed that the biological functions of differentially expressed mRNAs were associated with metabolism, development and inflammation. The results of realtime PCR validation were consistent with the data from the microarrays. Conclusions: Our data uncovered the expression profiles of lncRNAs and mRNAs in vivo and in vitro, which may help delineate the mechanisms of astrocyte activation during MS/EAE process. |
| first_indexed | 2024-12-10T22:36:18Z |
| format | Article |
| id | doaj.art-93f135c840cd49c58c5fc91704282f4c |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-10T22:36:18Z |
| publishDate | 2018-03-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-93f135c840cd49c58c5fc91704282f4c2022-12-22T01:30:53ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782018-03-014551986199810.1159/000487975487975Analysis of Long Noncoding RNA and mRNA Expression Profiles in IL-9-Activated Astrocytes and EAE MiceXiaomei LiuQing ZhangWeixiao WangDongjiao ZuoJing WangFeng ZhouLiping NiuXiangyang LiSuping QinYanbo KouFanyun KongWei PanYugang WangDianshuai GaoHong SunJessica M. MevesKuiyang ZhengRenxian TangBackground/Aims: Multiple sclerosis (MS) is an autoimmune disease in the central nervous system associated with demyelination and axonal injury. Astrocyte activation is involved in the pathogenesis of MS and experimental autoimmune encephalomyelitis (EAE), an animal model of MS. This study was designed to find potential lncRNAs in EAE mice and activated astrocytes. Methods: we performed microarray analysis of lncRNAs from the brain tissues of EAE mice and primary mouse astrocytes treated with IL-9(50 ng/ml). 12 lncRNAs were validated through real-time PCR. Gene ontology and KEGG pathway analysis were applied to explore the potential functions of lncRNAs. Results: Differentially expressed 3300 lncRNAs and 3250 mRNAs were in the brain tissues of EAE mice, and 3748 lncRNAs and 3332 mRNAs were in activated astrocytes. Notably, there were 2 co-up-regulated lncRNAs and 3 co-down-regulated lncRNAs both in the brain tissues of EAE mice and in activated astrocytes, including Gm14005, Gm12478, mouselincRNA1117, AK080435, and mouselincRNA0681, which regulate the ER calcium flux kinetics, zinc finger protein and cell apoptosis. Similarly, there were 7 mRNAs co-up-regulated and 2 mRNAs co-down-regulated both in vivo and in vitro. Gene ontology and KEGG pathway analysis showed that the biological functions of differentially expressed mRNAs were associated with metabolism, development and inflammation. The results of realtime PCR validation were consistent with the data from the microarrays. Conclusions: Our data uncovered the expression profiles of lncRNAs and mRNAs in vivo and in vitro, which may help delineate the mechanisms of astrocyte activation during MS/EAE process.https://www.karger.com/Article/FullText/487975Multiple sclerosisExperimental autoimmune encephalomyelitisLncRNAsAstrocytesIL-9 |
| spellingShingle | Xiaomei Liu Qing Zhang Weixiao Wang Dongjiao Zuo Jing Wang Feng Zhou Liping Niu Xiangyang Li Suping Qin Yanbo Kou Fanyun Kong Wei Pan Yugang Wang Dianshuai Gao Hong Sun Jessica M. Meves Kuiyang Zheng Renxian Tang Analysis of Long Noncoding RNA and mRNA Expression Profiles in IL-9-Activated Astrocytes and EAE Mice Cellular Physiology and Biochemistry Multiple sclerosis Experimental autoimmune encephalomyelitis LncRNAs Astrocytes IL-9 |
| title | Analysis of Long Noncoding RNA and mRNA Expression Profiles in IL-9-Activated Astrocytes and EAE Mice |
| title_full | Analysis of Long Noncoding RNA and mRNA Expression Profiles in IL-9-Activated Astrocytes and EAE Mice |
| title_fullStr | Analysis of Long Noncoding RNA and mRNA Expression Profiles in IL-9-Activated Astrocytes and EAE Mice |
| title_full_unstemmed | Analysis of Long Noncoding RNA and mRNA Expression Profiles in IL-9-Activated Astrocytes and EAE Mice |
| title_short | Analysis of Long Noncoding RNA and mRNA Expression Profiles in IL-9-Activated Astrocytes and EAE Mice |
| title_sort | analysis of long noncoding rna and mrna expression profiles in il 9 activated astrocytes and eae mice |
| topic | Multiple sclerosis Experimental autoimmune encephalomyelitis LncRNAs Astrocytes IL-9 |
| url | https://www.karger.com/Article/FullText/487975 |
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