Preparation, statistical optimization and in vitro characterization of solid lipid nanoparticles as a potential vehicle for transdermal delivery of tramadol hydrochloride as a hydrophilic Compound
As encapsulation of hydrophilic drugs in the solid lipid nanoparticles (SLNs) is still a challenging issue, the aim of this study was to prepare SLNs containing tramadol hydrochloride as a hydrophilic compound.The SLNs were prepared using glycerol monostearate (GMS), soy lecithin and tween 80 by dou...
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| Format: | Article |
| Language: | English |
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Iranian Society of Nanomedicine
2020-04-01
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| Series: | Nanomedicine Research Journal |
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| Online Access: | http://www.nanomedicine-rj.com/article_40563_1ee307fea7f1d2da01d1c319fc52ea9b.pdf |
| _version_ | 1798017381370429440 |
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| author | Mina Abbasnia Ali Reza Vatanara Reza Mahjub |
| author_facet | Mina Abbasnia Ali Reza Vatanara Reza Mahjub |
| author_sort | Mina Abbasnia |
| collection | DOAJ |
| description | As encapsulation of hydrophilic drugs in the solid lipid nanoparticles (SLNs) is still a challenging issue, the aim of this study was to prepare SLNs containing tramadol hydrochloride as a hydrophilic compound.The SLNs were prepared using glycerol monostearate (GMS), soy lecithin and tween 80 by double emulsification-solvent evaporation technique. The nanoparticles were optimized through a central-composite response surface (RSM) method. The independent variables were GMS/lecithin ratio and the amount of drug while dependent responses were size, polydispersity index (PdI) and zeta potential. The optimized nanoparticles were then freeze dried and their morphology was examined using transmission electron microscopy (TEM). Finally, the in vitro drug release profile from nanoparticles was evaluated and the kinetic of the release was determined. The particle size, PdI, zeta potential, entrapment efficiency and loading efficiency of the optimized SLNs were 13117.25 nm, 0.210.013, -11.2 1.04 mV, 89.42.38% and 9.49±0.14%, respectively. TEM images revealed de-agglomerated spherical nanoparticles. In vitro release studies showed sustained release of tramadol over 72 h and the release kinetic was best fitted to the first order and Korsmeyer-Peppas kinetic model. The obtained results indicated that tramadol as a hydrophilic drug can appropriately entrap in the solid lipid nanoparticles exhibiting favorable physico-chemical properties. |
| first_indexed | 2024-04-11T16:06:22Z |
| format | Article |
| id | doaj.art-93f17d7ebb144033bc27d8f7a64b3159 |
| institution | Directory Open Access Journal |
| issn | 2476-3489 2476-7123 |
| language | English |
| last_indexed | 2024-04-11T16:06:22Z |
| publishDate | 2020-04-01 |
| publisher | Iranian Society of Nanomedicine |
| record_format | Article |
| series | Nanomedicine Research Journal |
| spelling | doaj.art-93f17d7ebb144033bc27d8f7a64b31592022-12-22T04:14:49ZengIranian Society of NanomedicineNanomedicine Research Journal2476-34892476-71232020-04-015212013110.22034/nmrj.2020.02.00340563Preparation, statistical optimization and in vitro characterization of solid lipid nanoparticles as a potential vehicle for transdermal delivery of tramadol hydrochloride as a hydrophilic CompoundMina Abbasnia0Ali Reza Vatanara1Reza Mahjub2Department of Pharmaceutics, Faculty of pharmacy, Hamedan University of Medical Science, Hamedan, IranDepartment of Pharmaceutics, Faculty of Pharmacy, Tehran University of Medical Science, Tehran, IranDepartment of Pharmaceutics, Faculty of pharmacy, Hamedan University of Medical Science, Hamedan, IranAs encapsulation of hydrophilic drugs in the solid lipid nanoparticles (SLNs) is still a challenging issue, the aim of this study was to prepare SLNs containing tramadol hydrochloride as a hydrophilic compound.The SLNs were prepared using glycerol monostearate (GMS), soy lecithin and tween 80 by double emulsification-solvent evaporation technique. The nanoparticles were optimized through a central-composite response surface (RSM) method. The independent variables were GMS/lecithin ratio and the amount of drug while dependent responses were size, polydispersity index (PdI) and zeta potential. The optimized nanoparticles were then freeze dried and their morphology was examined using transmission electron microscopy (TEM). Finally, the in vitro drug release profile from nanoparticles was evaluated and the kinetic of the release was determined. The particle size, PdI, zeta potential, entrapment efficiency and loading efficiency of the optimized SLNs were 13117.25 nm, 0.210.013, -11.2 1.04 mV, 89.42.38% and 9.49±0.14%, respectively. TEM images revealed de-agglomerated spherical nanoparticles. In vitro release studies showed sustained release of tramadol over 72 h and the release kinetic was best fitted to the first order and Korsmeyer-Peppas kinetic model. The obtained results indicated that tramadol as a hydrophilic drug can appropriately entrap in the solid lipid nanoparticles exhibiting favorable physico-chemical properties.http://www.nanomedicine-rj.com/article_40563_1ee307fea7f1d2da01d1c319fc52ea9b.pdftramadol hydrochloridehydrophilic drugsolid lipid nanoparticles (sln)double emulsification-solvent evaporation techniquecentral-composite response surface methodologytransdermal delivery |
| spellingShingle | Mina Abbasnia Ali Reza Vatanara Reza Mahjub Preparation, statistical optimization and in vitro characterization of solid lipid nanoparticles as a potential vehicle for transdermal delivery of tramadol hydrochloride as a hydrophilic Compound Nanomedicine Research Journal tramadol hydrochloride hydrophilic drug solid lipid nanoparticles (sln) double emulsification-solvent evaporation technique central-composite response surface methodology transdermal delivery |
| title | Preparation, statistical optimization and in vitro characterization of solid lipid nanoparticles as a potential vehicle for transdermal delivery of tramadol hydrochloride as a hydrophilic Compound |
| title_full | Preparation, statistical optimization and in vitro characterization of solid lipid nanoparticles as a potential vehicle for transdermal delivery of tramadol hydrochloride as a hydrophilic Compound |
| title_fullStr | Preparation, statistical optimization and in vitro characterization of solid lipid nanoparticles as a potential vehicle for transdermal delivery of tramadol hydrochloride as a hydrophilic Compound |
| title_full_unstemmed | Preparation, statistical optimization and in vitro characterization of solid lipid nanoparticles as a potential vehicle for transdermal delivery of tramadol hydrochloride as a hydrophilic Compound |
| title_short | Preparation, statistical optimization and in vitro characterization of solid lipid nanoparticles as a potential vehicle for transdermal delivery of tramadol hydrochloride as a hydrophilic Compound |
| title_sort | preparation statistical optimization and in vitro characterization of solid lipid nanoparticles as a potential vehicle for transdermal delivery of tramadol hydrochloride as a hydrophilic compound |
| topic | tramadol hydrochloride hydrophilic drug solid lipid nanoparticles (sln) double emulsification-solvent evaporation technique central-composite response surface methodology transdermal delivery |
| url | http://www.nanomedicine-rj.com/article_40563_1ee307fea7f1d2da01d1c319fc52ea9b.pdf |
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