Response surface methodology to evaluation the recovery of amylases by hollow fiber membrane
This work aimed to study the pH and the transmembrane pressure effects during the recovery of alpha and beta amylases enzymes from corn malt (Zea mays) by hollow fiber membrane. The optimal condition was obtained for a statistical model, established by response surface methodology (RSM). The respons...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2007-07-01
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| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000400017 |
| _version_ | 1818695550108696576 |
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| author | João Baptista Severo Júnior Laura Sampaio de Sá Oliveira Fernanda Silva Sardeiro Roberto Rodrigues de Souza Francisco Luiz Gumes Lopes José Carlos Curvelo Santana Elias Basile Tambourgi |
| author_facet | João Baptista Severo Júnior Laura Sampaio de Sá Oliveira Fernanda Silva Sardeiro Roberto Rodrigues de Souza Francisco Luiz Gumes Lopes José Carlos Curvelo Santana Elias Basile Tambourgi |
| author_sort | João Baptista Severo Júnior |
| collection | DOAJ |
| description | This work aimed to study the pH and the transmembrane pressure effects during the recovery of alpha and beta amylases enzymes from corn malt (Zea mays) by hollow fiber membrane. The optimal condition was obtained for a statistical model, established by response surface methodology (RSM). The response surface analysis showed that the best operation condition for amylolitics enzymes recovery by hollow fiber membrane was 0.05 bar and pH 5.00, while the enzymes were purified about of 26 times.<br>Este trabalho objetivou estudar o efeito do pH e da pressão trans-membrana durante a recuperação das enzimas alfa e beta amilases do malte de milho (Zea mays) por membranas de fibras ocas, a obtenção das condições ótimas foi feita por um modelo estatístico, estabelecido pela metodologia de superfície de resposta (RSM). A análise da superfície de resposta mostrou que as melhores condições operacionais para a recuperação das enzimas amiloliticas por membranas de fibras ocas foi 0,05 bar e pH 5,00; onde as enzimas foram purificadas cerca de 26 vezes. |
| first_indexed | 2024-12-17T13:47:15Z |
| format | Article |
| id | doaj.art-93f1c33e28b84a9088b16eab9bf6eb02 |
| institution | Directory Open Access Journal |
| issn | 1516-8913 1678-4324 |
| language | English |
| last_indexed | 2024-12-17T13:47:15Z |
| publishDate | 2007-07-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-93f1c33e28b84a9088b16eab9bf6eb022022-12-21T21:46:07ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242007-07-0150471371810.1590/S1516-89132007000400017Response surface methodology to evaluation the recovery of amylases by hollow fiber membraneJoão Baptista Severo JúniorLaura Sampaio de Sá OliveiraFernanda Silva SardeiroRoberto Rodrigues de SouzaFrancisco Luiz Gumes LopesJosé Carlos Curvelo SantanaElias Basile TambourgiThis work aimed to study the pH and the transmembrane pressure effects during the recovery of alpha and beta amylases enzymes from corn malt (Zea mays) by hollow fiber membrane. The optimal condition was obtained for a statistical model, established by response surface methodology (RSM). The response surface analysis showed that the best operation condition for amylolitics enzymes recovery by hollow fiber membrane was 0.05 bar and pH 5.00, while the enzymes were purified about of 26 times.<br>Este trabalho objetivou estudar o efeito do pH e da pressão trans-membrana durante a recuperação das enzimas alfa e beta amilases do malte de milho (Zea mays) por membranas de fibras ocas, a obtenção das condições ótimas foi feita por um modelo estatístico, estabelecido pela metodologia de superfície de resposta (RSM). A análise da superfície de resposta mostrou que as melhores condições operacionais para a recuperação das enzimas amiloliticas por membranas de fibras ocas foi 0,05 bar e pH 5,00; onde as enzimas foram purificadas cerca de 26 vezes.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000400017Hollow fiber membranecorn maltalpha and beta amylaseRSM |
| spellingShingle | João Baptista Severo Júnior Laura Sampaio de Sá Oliveira Fernanda Silva Sardeiro Roberto Rodrigues de Souza Francisco Luiz Gumes Lopes José Carlos Curvelo Santana Elias Basile Tambourgi Response surface methodology to evaluation the recovery of amylases by hollow fiber membrane Brazilian Archives of Biology and Technology Hollow fiber membrane corn malt alpha and beta amylase RSM |
| title | Response surface methodology to evaluation the recovery of amylases by hollow fiber membrane |
| title_full | Response surface methodology to evaluation the recovery of amylases by hollow fiber membrane |
| title_fullStr | Response surface methodology to evaluation the recovery of amylases by hollow fiber membrane |
| title_full_unstemmed | Response surface methodology to evaluation the recovery of amylases by hollow fiber membrane |
| title_short | Response surface methodology to evaluation the recovery of amylases by hollow fiber membrane |
| title_sort | response surface methodology to evaluation the recovery of amylases by hollow fiber membrane |
| topic | Hollow fiber membrane corn malt alpha and beta amylase RSM |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000400017 |
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