Detection of the Xanthi Chryso-like Virus in New Geographical Area and a Novel Arthropod Carrier
Here, we report on a serendipitous finding of a chryso-like virus associated with <i>Culex pipiens</i> mosquitos in the course of study aimed to detect and characterize West Nile virus (WNV) circulating in mosquitos in Serbia, Southern Europe. Upon initial detection of unexpected product...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Tropical Medicine and Infectious Disease |
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| Online Access: | https://www.mdpi.com/2414-6366/8/4/225 |
| _version_ | 1827743525374001152 |
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| author | Marko Jankovic Valentina Cirkovic Gorana Stamenkovic Ana Loncar Marija Todorovic Maja Stanojevic Marina Siljic |
| author_facet | Marko Jankovic Valentina Cirkovic Gorana Stamenkovic Ana Loncar Marija Todorovic Maja Stanojevic Marina Siljic |
| author_sort | Marko Jankovic |
| collection | DOAJ |
| description | Here, we report on a serendipitous finding of a chryso-like virus associated with <i>Culex pipiens</i> mosquitos in the course of study aimed to detect and characterize West Nile virus (WNV) circulating in mosquitos in Serbia, Southern Europe. Upon initial detection of unexpected product in a PCR protocol for partial WNV <i>NS5</i> gene amplification, further confirmation and identification was obtained through additional PCR and Sanger sequencing experiments. Bioinformatic and phylogenetic analysis identified the obtained sequences as Xanthi chryso-like virus (XCLV). The finding is particular for the fact that it associates XCLV with a new potential vector species and documents a novel geographical area of its distribution. |
| first_indexed | 2024-03-11T04:28:14Z |
| format | Article |
| id | doaj.art-93f24ca1ee3d49188de9752b69a97c38 |
| institution | Directory Open Access Journal |
| issn | 2414-6366 |
| language | English |
| last_indexed | 2024-03-11T04:28:14Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Tropical Medicine and Infectious Disease |
| spelling | doaj.art-93f24ca1ee3d49188de9752b69a97c382023-11-17T21:39:51ZengMDPI AGTropical Medicine and Infectious Disease2414-63662023-04-018422510.3390/tropicalmed8040225Detection of the Xanthi Chryso-like Virus in New Geographical Area and a Novel Arthropod CarrierMarko Jankovic0Valentina Cirkovic1Gorana Stamenkovic2Ana Loncar3Marija Todorovic4Maja Stanojevic5Marina Siljic6Faculty of Medicine, Institute of Microbiology and Immunology, Department of Virology, University of Belgrade, 1 Dr Subotića Starijeg Street, 11000 Belgrade, SerbiaGroup for Medical Entomology, Centre of Excellence for Food and Vector Borne Zoonoses, Institute for Medical Research, University of Belgrade, 11000 Belgrade, SerbiaDepartment for Genetic Research, Institute for Biological Research “Siniša Stanković”—National Institute of Republic of Serbia, University of Belgrade, 11000 Belgrade, SerbiaInstitute for Biocides and Medical Ecology, 11000 Belgrade, SerbiaFaculty of Medicine, Institute of Microbiology and Immunology, Department of Virology, University of Belgrade, 1 Dr Subotića Starijeg Street, 11000 Belgrade, SerbiaFaculty of Medicine, Institute of Microbiology and Immunology, Department of Virology, University of Belgrade, 1 Dr Subotića Starijeg Street, 11000 Belgrade, SerbiaFaculty of Medicine, Institute of Microbiology and Immunology, Department of Virology, University of Belgrade, 1 Dr Subotića Starijeg Street, 11000 Belgrade, SerbiaHere, we report on a serendipitous finding of a chryso-like virus associated with <i>Culex pipiens</i> mosquitos in the course of study aimed to detect and characterize West Nile virus (WNV) circulating in mosquitos in Serbia, Southern Europe. Upon initial detection of unexpected product in a PCR protocol for partial WNV <i>NS5</i> gene amplification, further confirmation and identification was obtained through additional PCR and Sanger sequencing experiments. Bioinformatic and phylogenetic analysis identified the obtained sequences as Xanthi chryso-like virus (XCLV). The finding is particular for the fact that it associates XCLV with a new potential vector species and documents a novel geographical area of its distribution.https://www.mdpi.com/2414-6366/8/4/225<i>Culex pipiens</i>mosquitoarthropod-borneRNA virusmycoviruschrysovirus |
| spellingShingle | Marko Jankovic Valentina Cirkovic Gorana Stamenkovic Ana Loncar Marija Todorovic Maja Stanojevic Marina Siljic Detection of the Xanthi Chryso-like Virus in New Geographical Area and a Novel Arthropod Carrier Tropical Medicine and Infectious Disease <i>Culex pipiens</i> mosquito arthropod-borne RNA virus mycovirus chrysovirus |
| title | Detection of the Xanthi Chryso-like Virus in New Geographical Area and a Novel Arthropod Carrier |
| title_full | Detection of the Xanthi Chryso-like Virus in New Geographical Area and a Novel Arthropod Carrier |
| title_fullStr | Detection of the Xanthi Chryso-like Virus in New Geographical Area and a Novel Arthropod Carrier |
| title_full_unstemmed | Detection of the Xanthi Chryso-like Virus in New Geographical Area and a Novel Arthropod Carrier |
| title_short | Detection of the Xanthi Chryso-like Virus in New Geographical Area and a Novel Arthropod Carrier |
| title_sort | detection of the xanthi chryso like virus in new geographical area and a novel arthropod carrier |
| topic | <i>Culex pipiens</i> mosquito arthropod-borne RNA virus mycovirus chrysovirus |
| url | https://www.mdpi.com/2414-6366/8/4/225 |
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