A Sensitive Voltammetric Approach Employing a Bare Boron-Doped Diamond Electrode as a Sensor for the Determination of Hydroxocobalamin

The voltammetric behavior of hydroxocobalamin (OH-CBL) was firstly studied by employing a bare boron-doped diamond electrode as a working electrode. It was found that OH-CBL provided four anodic signals on BDDE in acidic supporting electrolytes and one cathodic signal. The anodic peak situated at +4...

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Main Authors: Lenka Janíková, Renáta Šelešovská, Iveta Stýblová, Jaromíra Chýlková
Format: Article
Language:English
Published: MDPI AG 2023-09-01
Series:Engineering Proceedings
Subjects:
Online Access:https://www.mdpi.com/2673-4591/48/1/7
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author Lenka Janíková
Renáta Šelešovská
Iveta Stýblová
Jaromíra Chýlková
author_facet Lenka Janíková
Renáta Šelešovská
Iveta Stýblová
Jaromíra Chýlková
author_sort Lenka Janíková
collection DOAJ
description The voltammetric behavior of hydroxocobalamin (OH-CBL) was firstly studied by employing a bare boron-doped diamond electrode as a working electrode. It was found that OH-CBL provided four anodic signals on BDDE in acidic supporting electrolytes and one cathodic signal. The anodic peak situated at +412 mV (<i>vs.</i> Ag|AgCl|KCl (sat.) recorded in 0.1 mol/L H<sub>2</sub>SO<sub>4</sub>) was found to be suitable for analytical purposes due to its position and shape. A novel voltammetric approach based on differential pulse voltammetry was developed and it was found as a sensitive analytical tool, with low limit of detection (LD = 13.2 nmol/L), applicable in analysis of vitamin preparations.
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spelling doaj.art-93f4bfd9d82246f3b63220c96377d4482024-03-27T13:36:02ZengMDPI AGEngineering Proceedings2673-45912023-09-01481710.3390/CSAC2023-14894A Sensitive Voltammetric Approach Employing a Bare Boron-Doped Diamond Electrode as a Sensor for the Determination of HydroxocobalaminLenka Janíková0Renáta Šelešovská1Iveta Stýblová2Jaromíra Chýlková3Institute of Environmental and Chemical Engineering, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 532 10 Pardubice, Czech RepublicInstitute of Environmental and Chemical Engineering, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 532 10 Pardubice, Czech RepublicInstitute of Environmental and Chemical Engineering, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 532 10 Pardubice, Czech RepublicInstitute of Environmental and Chemical Engineering, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 532 10 Pardubice, Czech RepublicThe voltammetric behavior of hydroxocobalamin (OH-CBL) was firstly studied by employing a bare boron-doped diamond electrode as a working electrode. It was found that OH-CBL provided four anodic signals on BDDE in acidic supporting electrolytes and one cathodic signal. The anodic peak situated at +412 mV (<i>vs.</i> Ag|AgCl|KCl (sat.) recorded in 0.1 mol/L H<sub>2</sub>SO<sub>4</sub>) was found to be suitable for analytical purposes due to its position and shape. A novel voltammetric approach based on differential pulse voltammetry was developed and it was found as a sensitive analytical tool, with low limit of detection (LD = 13.2 nmol/L), applicable in analysis of vitamin preparations.https://www.mdpi.com/2673-4591/48/1/7hydroxocobalaminvitamin B12boron-doped diamond electrodevoltammetryvitamin analysis
spellingShingle Lenka Janíková
Renáta Šelešovská
Iveta Stýblová
Jaromíra Chýlková
A Sensitive Voltammetric Approach Employing a Bare Boron-Doped Diamond Electrode as a Sensor for the Determination of Hydroxocobalamin
Engineering Proceedings
hydroxocobalamin
vitamin B12
boron-doped diamond electrode
voltammetry
vitamin analysis
title A Sensitive Voltammetric Approach Employing a Bare Boron-Doped Diamond Electrode as a Sensor for the Determination of Hydroxocobalamin
title_full A Sensitive Voltammetric Approach Employing a Bare Boron-Doped Diamond Electrode as a Sensor for the Determination of Hydroxocobalamin
title_fullStr A Sensitive Voltammetric Approach Employing a Bare Boron-Doped Diamond Electrode as a Sensor for the Determination of Hydroxocobalamin
title_full_unstemmed A Sensitive Voltammetric Approach Employing a Bare Boron-Doped Diamond Electrode as a Sensor for the Determination of Hydroxocobalamin
title_short A Sensitive Voltammetric Approach Employing a Bare Boron-Doped Diamond Electrode as a Sensor for the Determination of Hydroxocobalamin
title_sort sensitive voltammetric approach employing a bare boron doped diamond electrode as a sensor for the determination of hydroxocobalamin
topic hydroxocobalamin
vitamin B12
boron-doped diamond electrode
voltammetry
vitamin analysis
url https://www.mdpi.com/2673-4591/48/1/7
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