In Vivo Effect of Resveratrol-Loaded Solid Lipid Nanoparticles to Relieve Physical Fatigue for Sports Nutrition Supplements
Resveratrol (RSV) is a natural flavonoid polyphenol compound extracted from the plants which shows various biological activities. However, the clinical application of RSV is limited by its poor aqueous solubility, rapid metabolism and poor bioavailability. In this study, resveratrol-loaded solid lip...
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| Format: | Article |
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MDPI AG
2020-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/22/5302 |
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| author | Lili Qin Tianfeng Lu Yao Qin Yiwei He Ningxin Cui Ai Du Jingyu Sun |
| author_facet | Lili Qin Tianfeng Lu Yao Qin Yiwei He Ningxin Cui Ai Du Jingyu Sun |
| author_sort | Lili Qin |
| collection | DOAJ |
| description | Resveratrol (RSV) is a natural flavonoid polyphenol compound extracted from the plants which shows various biological activities. However, the clinical application of RSV is limited by its poor aqueous solubility, rapid metabolism and poor bioavailability. In this study, resveratrol-loaded solid lipid nanoparticles (RSV- SLNs) was design as a nano-antioxidant against the physical fatigue. The resultant RSV-SLNs were characterized by photon correlation spectroscopy (PCS), transmission electron micrographs (TEM), zeta potential, differential scanning calorimetry (DSC) and Raman spectroscopy pattern. Furthermore, the in vivo anti-fatigue effect assays showed that RSV-SLNs prolonged the mice exhausted time and running distance. The biochemical parameters of blood related to fatigue suggested that RSV-SLNs have potential applications to improve the antioxidant defense of the mice after extensive exercise and confer anti-fatigue capability. Furthermore, the molecular mechanisms of antioxidant by RSV-SLNs supplementation was investigated through the analysis of silent information regulator 2 homolog 1 (SIRT1) protein expression, which demonstrated that it could downregulate the expression of SIRT1 and increase autophagy markers, microtubule-associated protein 1 light chain 3-II (LC3-II) and sequestosome-1 (SQSTM1/p62). These results reveal that the RSV-SLNs may have great potential used as a novel anti-fatigue sports nutritional supplement. |
| first_indexed | 2024-03-10T14:51:59Z |
| format | Article |
| id | doaj.art-93f6c2ec67fa4df784dd210bc9b998e9 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T14:51:59Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-93f6c2ec67fa4df784dd210bc9b998e92023-11-20T20:53:30ZengMDPI AGMolecules1420-30492020-11-012522530210.3390/molecules25225302In Vivo Effect of Resveratrol-Loaded Solid Lipid Nanoparticles to Relieve Physical Fatigue for Sports Nutrition SupplementsLili Qin0Tianfeng Lu1Yao Qin2Yiwei He3Ningxin Cui4Ai Du5Jingyu Sun6Sports and Health Research Center, Department of Physical Education, Tongji University, Shanghai 200092, ChinaSports and Health Research Center, Department of Physical Education, Tongji University, Shanghai 200092, ChinaSports and Health Research Center, Department of Physical Education, Tongji University, Shanghai 200092, ChinaSports and Health Research Center, Department of Physical Education, Tongji University, Shanghai 200092, ChinaSports and Health Research Center, Department of Physical Education, Tongji University, Shanghai 200092, ChinaSchool of Physics Science and Engineering, Tongji University, Shanghai 200092, ChinaSports and Health Research Center, Department of Physical Education, Tongji University, Shanghai 200092, ChinaResveratrol (RSV) is a natural flavonoid polyphenol compound extracted from the plants which shows various biological activities. However, the clinical application of RSV is limited by its poor aqueous solubility, rapid metabolism and poor bioavailability. In this study, resveratrol-loaded solid lipid nanoparticles (RSV- SLNs) was design as a nano-antioxidant against the physical fatigue. The resultant RSV-SLNs were characterized by photon correlation spectroscopy (PCS), transmission electron micrographs (TEM), zeta potential, differential scanning calorimetry (DSC) and Raman spectroscopy pattern. Furthermore, the in vivo anti-fatigue effect assays showed that RSV-SLNs prolonged the mice exhausted time and running distance. The biochemical parameters of blood related to fatigue suggested that RSV-SLNs have potential applications to improve the antioxidant defense of the mice after extensive exercise and confer anti-fatigue capability. Furthermore, the molecular mechanisms of antioxidant by RSV-SLNs supplementation was investigated through the analysis of silent information regulator 2 homolog 1 (SIRT1) protein expression, which demonstrated that it could downregulate the expression of SIRT1 and increase autophagy markers, microtubule-associated protein 1 light chain 3-II (LC3-II) and sequestosome-1 (SQSTM1/p62). These results reveal that the RSV-SLNs may have great potential used as a novel anti-fatigue sports nutritional supplement.https://www.mdpi.com/1420-3049/25/22/5302solid lipid nanoparticles (SLNs)resveratroldrug deliveryanti-fatiguenutrition supplements |
| spellingShingle | Lili Qin Tianfeng Lu Yao Qin Yiwei He Ningxin Cui Ai Du Jingyu Sun In Vivo Effect of Resveratrol-Loaded Solid Lipid Nanoparticles to Relieve Physical Fatigue for Sports Nutrition Supplements Molecules solid lipid nanoparticles (SLNs) resveratrol drug delivery anti-fatigue nutrition supplements |
| title | In Vivo Effect of Resveratrol-Loaded Solid Lipid Nanoparticles to Relieve Physical Fatigue for Sports Nutrition Supplements |
| title_full | In Vivo Effect of Resveratrol-Loaded Solid Lipid Nanoparticles to Relieve Physical Fatigue for Sports Nutrition Supplements |
| title_fullStr | In Vivo Effect of Resveratrol-Loaded Solid Lipid Nanoparticles to Relieve Physical Fatigue for Sports Nutrition Supplements |
| title_full_unstemmed | In Vivo Effect of Resveratrol-Loaded Solid Lipid Nanoparticles to Relieve Physical Fatigue for Sports Nutrition Supplements |
| title_short | In Vivo Effect of Resveratrol-Loaded Solid Lipid Nanoparticles to Relieve Physical Fatigue for Sports Nutrition Supplements |
| title_sort | in vivo effect of resveratrol loaded solid lipid nanoparticles to relieve physical fatigue for sports nutrition supplements |
| topic | solid lipid nanoparticles (SLNs) resveratrol drug delivery anti-fatigue nutrition supplements |
| url | https://www.mdpi.com/1420-3049/25/22/5302 |
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