Long-term ethanol intoxication reduces inflammatory responses in rats
The anti-inflammatory effects of long-term ethanol intoxication were determined during ethanol treatment and withdrawal on the basis of neutrophil and eosinophil migration, hind paw edema and mast cell degranulation. Male Wistar rats (180-200 g, around 2 months of age) were exposed to increasing con...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2005-01-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100013 |
| _version_ | 1828737742402486272 |
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| author | E.M. Carvalho G.A.C. Brito B.B.G.P. Pessoa R.A. Ribeiro F.R. Capaz |
| author_facet | E.M. Carvalho G.A.C. Brito B.B.G.P. Pessoa R.A. Ribeiro F.R. Capaz |
| author_sort | E.M. Carvalho |
| collection | DOAJ |
| description | The anti-inflammatory effects of long-term ethanol intoxication were determined during ethanol treatment and withdrawal on the basis of neutrophil and eosinophil migration, hind paw edema and mast cell degranulation. Male Wistar rats (180-200 g, around 2 months of age) were exposed to increasing concentrations of ethanol vapor over a 10-day period. One group was evaluated immediately after exposure (treated group - intoxicated), and another was studied 7 h later (withdrawal group). Ethanol inhalation treatment significantly inhibited carrageenan- (62% for the intoxicated group, N = 5, and 35% for the withdrawal group, N = 6) and dextran-induced paw edema (32% for intoxicated rats and 26% for withdrawal rats, N = 5 per group). Ethanol inhalation significantly reduced carrageenan-induced neutrophil migration (95% for intoxicated rats and 41% for withdrawn rats, N = 6 per group) into a subcutaneous 6-day-old air pouch, and Sephadex-induced eosinophil migration to the rat peritoneal cavity (100% for intoxicated rats and 64% for withdrawn rats, N = 6 per group). A significant decrease of mast cell degranulation was also demonstrated (control, 82%; intoxicated, 49%; withdrawn, 51%, N = 6, 6 and 8, respectively). Total leukocyte and neutrophil counts in venous blood increased significantly during the 10 days of ethanol inhalation (leukocytes, 13, 27 and 40%; neutrophils, 42, 238 and 252%, respectively, on days 5, 9 and 10, N = 7, 6 and 6). The cell counts decreased during withdrawal, but were still significantly elevated (leukocytes, 10%; neutrophils, 246%, N = 6). These findings indicate that both the cellular and vascular components of the inflammatory response are compromised by long-term ethanol intoxication and remain reduced during the withdrawal period. |
| first_indexed | 2024-04-12T23:50:22Z |
| format | Article |
| id | doaj.art-93fc1555183a42b68961484d9cd6dacf |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-04-12T23:50:22Z |
| publishDate | 2005-01-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-93fc1555183a42b68961484d9cd6dacf2022-12-22T03:11:43ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2005-01-01381818910.1590/S0100-879X2005000100013Long-term ethanol intoxication reduces inflammatory responses in ratsE.M. CarvalhoG.A.C. BritoB.B.G.P. PessoaR.A. RibeiroF.R. CapazThe anti-inflammatory effects of long-term ethanol intoxication were determined during ethanol treatment and withdrawal on the basis of neutrophil and eosinophil migration, hind paw edema and mast cell degranulation. Male Wistar rats (180-200 g, around 2 months of age) were exposed to increasing concentrations of ethanol vapor over a 10-day period. One group was evaluated immediately after exposure (treated group - intoxicated), and another was studied 7 h later (withdrawal group). Ethanol inhalation treatment significantly inhibited carrageenan- (62% for the intoxicated group, N = 5, and 35% for the withdrawal group, N = 6) and dextran-induced paw edema (32% for intoxicated rats and 26% for withdrawal rats, N = 5 per group). Ethanol inhalation significantly reduced carrageenan-induced neutrophil migration (95% for intoxicated rats and 41% for withdrawn rats, N = 6 per group) into a subcutaneous 6-day-old air pouch, and Sephadex-induced eosinophil migration to the rat peritoneal cavity (100% for intoxicated rats and 64% for withdrawn rats, N = 6 per group). A significant decrease of mast cell degranulation was also demonstrated (control, 82%; intoxicated, 49%; withdrawn, 51%, N = 6, 6 and 8, respectively). Total leukocyte and neutrophil counts in venous blood increased significantly during the 10 days of ethanol inhalation (leukocytes, 13, 27 and 40%; neutrophils, 42, 238 and 252%, respectively, on days 5, 9 and 10, N = 7, 6 and 6). The cell counts decreased during withdrawal, but were still significantly elevated (leukocytes, 10%; neutrophils, 246%, N = 6). These findings indicate that both the cellular and vascular components of the inflammatory response are compromised by long-term ethanol intoxication and remain reduced during the withdrawal period.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100013EthanolInflammationCarrageenanDextranAir-pouchMast cells |
| spellingShingle | E.M. Carvalho G.A.C. Brito B.B.G.P. Pessoa R.A. Ribeiro F.R. Capaz Long-term ethanol intoxication reduces inflammatory responses in rats Brazilian Journal of Medical and Biological Research Ethanol Inflammation Carrageenan Dextran Air-pouch Mast cells |
| title | Long-term ethanol intoxication reduces inflammatory responses in rats |
| title_full | Long-term ethanol intoxication reduces inflammatory responses in rats |
| title_fullStr | Long-term ethanol intoxication reduces inflammatory responses in rats |
| title_full_unstemmed | Long-term ethanol intoxication reduces inflammatory responses in rats |
| title_short | Long-term ethanol intoxication reduces inflammatory responses in rats |
| title_sort | long term ethanol intoxication reduces inflammatory responses in rats |
| topic | Ethanol Inflammation Carrageenan Dextran Air-pouch Mast cells |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100013 |
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