Comparative Transcriptome Analysis Provides Insights into the Molecular Mechanism Underlying the Effect of MeJA Treatment on the Biosynthesis of Saikosaponins in <i>Bupleurum chinense</i> DC.
<i>Bupleurum chinense</i> DC. is a well-known traditional Chinese medicinal plant that produces saikosaponins (SSs), which possess hepatoprotective, antipyretic, and anti-inflammatory activities. Methyl jasmonate (MeJA) is a signalling phytohormone that can increase the accumulation of S...
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MDPI AG
2023-02-01
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| author | Yanping Mao Yuping Yang Yuchan Li Yiguan Zhang Ping Wei Hua Chen Dabin Hou |
| author_facet | Yanping Mao Yuping Yang Yuchan Li Yiguan Zhang Ping Wei Hua Chen Dabin Hou |
| author_sort | Yanping Mao |
| collection | DOAJ |
| description | <i>Bupleurum chinense</i> DC. is a well-known traditional Chinese medicinal plant that produces saikosaponins (SSs), which possess hepatoprotective, antipyretic, and anti-inflammatory activities. Methyl jasmonate (MeJA) is a signalling phytohormone that can increase the accumulation of SSs in the root of <i>Bupleurum</i> plants. However, the molecular understanding of MeJA-mediated SS biosynthesis is not clear. Therefore, it is necessary to explore the molecular mechanism underlying the response of <i>B. chinense</i> DC. to MeJA in roots. In this study, we performed comparative transcriptome analysis of <i>B. chinense</i> DC. roots with different MeJA treatment times. In total, 104,057 unigenes were identified, of which 4053 were differentially expressed genes (DEGs). Most of the DEGs were downregulated after MeJA treatment, and GO enrichment analysis showed that they were mainly related to biological processes involved in stress responses and development. A total of 88 DEGs encoding enzymes known to be involved in the SS synthesis pathway were found, and most were significantly downregulated within 24 h. Based on the DEGs, 99 transcription factors (TFs) belonging to the AP2/ERF, WRKY, bZIP, ZFP, and bHLH families with different expression patterns were also identified. Further integrated analysis indicated that 20 DEGs involved in the SS synthesis pathway and 12 DEGs encoding TFs presented strong correlations with the SS contents, and these DEGs may be critical for the biosynthesis and regulation of SSs. These findings will be critical for further study of the response of <i>B. chinense</i> DC. to MeJA for SS biosynthesis. |
| first_indexed | 2024-03-11T08:32:38Z |
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| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-11T08:32:38Z |
| publishDate | 2023-02-01 |
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| series | Life |
| spelling | doaj.art-93fcb8aa42ad4149912786f5e5f224652023-11-16T21:42:59ZengMDPI AGLife2075-17292023-02-0113256310.3390/life13020563Comparative Transcriptome Analysis Provides Insights into the Molecular Mechanism Underlying the Effect of MeJA Treatment on the Biosynthesis of Saikosaponins in <i>Bupleurum chinense</i> DC.Yanping Mao0Yuping Yang1Yuchan Li2Yiguan Zhang3Ping Wei4Hua Chen5Dabin Hou6School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaSichuan Institute for Translational Chinese Medicine, Chengdu 610041, ChinaSichuan Institute for Translational Chinese Medicine, Chengdu 610041, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, ChinaSchool of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China<i>Bupleurum chinense</i> DC. is a well-known traditional Chinese medicinal plant that produces saikosaponins (SSs), which possess hepatoprotective, antipyretic, and anti-inflammatory activities. Methyl jasmonate (MeJA) is a signalling phytohormone that can increase the accumulation of SSs in the root of <i>Bupleurum</i> plants. However, the molecular understanding of MeJA-mediated SS biosynthesis is not clear. Therefore, it is necessary to explore the molecular mechanism underlying the response of <i>B. chinense</i> DC. to MeJA in roots. In this study, we performed comparative transcriptome analysis of <i>B. chinense</i> DC. roots with different MeJA treatment times. In total, 104,057 unigenes were identified, of which 4053 were differentially expressed genes (DEGs). Most of the DEGs were downregulated after MeJA treatment, and GO enrichment analysis showed that they were mainly related to biological processes involved in stress responses and development. A total of 88 DEGs encoding enzymes known to be involved in the SS synthesis pathway were found, and most were significantly downregulated within 24 h. Based on the DEGs, 99 transcription factors (TFs) belonging to the AP2/ERF, WRKY, bZIP, ZFP, and bHLH families with different expression patterns were also identified. Further integrated analysis indicated that 20 DEGs involved in the SS synthesis pathway and 12 DEGs encoding TFs presented strong correlations with the SS contents, and these DEGs may be critical for the biosynthesis and regulation of SSs. These findings will be critical for further study of the response of <i>B. chinense</i> DC. to MeJA for SS biosynthesis.https://www.mdpi.com/2075-1729/13/2/563comparative transcriptomeMeJA<i>Bupleurum chinense</i> DC.saikosaponin biosynthesisdifferentially expressed gene |
| spellingShingle | Yanping Mao Yuping Yang Yuchan Li Yiguan Zhang Ping Wei Hua Chen Dabin Hou Comparative Transcriptome Analysis Provides Insights into the Molecular Mechanism Underlying the Effect of MeJA Treatment on the Biosynthesis of Saikosaponins in <i>Bupleurum chinense</i> DC. Life comparative transcriptome MeJA <i>Bupleurum chinense</i> DC. saikosaponin biosynthesis differentially expressed gene |
| title | Comparative Transcriptome Analysis Provides Insights into the Molecular Mechanism Underlying the Effect of MeJA Treatment on the Biosynthesis of Saikosaponins in <i>Bupleurum chinense</i> DC. |
| title_full | Comparative Transcriptome Analysis Provides Insights into the Molecular Mechanism Underlying the Effect of MeJA Treatment on the Biosynthesis of Saikosaponins in <i>Bupleurum chinense</i> DC. |
| title_fullStr | Comparative Transcriptome Analysis Provides Insights into the Molecular Mechanism Underlying the Effect of MeJA Treatment on the Biosynthesis of Saikosaponins in <i>Bupleurum chinense</i> DC. |
| title_full_unstemmed | Comparative Transcriptome Analysis Provides Insights into the Molecular Mechanism Underlying the Effect of MeJA Treatment on the Biosynthesis of Saikosaponins in <i>Bupleurum chinense</i> DC. |
| title_short | Comparative Transcriptome Analysis Provides Insights into the Molecular Mechanism Underlying the Effect of MeJA Treatment on the Biosynthesis of Saikosaponins in <i>Bupleurum chinense</i> DC. |
| title_sort | comparative transcriptome analysis provides insights into the molecular mechanism underlying the effect of meja treatment on the biosynthesis of saikosaponins in i bupleurum chinense i dc |
| topic | comparative transcriptome MeJA <i>Bupleurum chinense</i> DC. saikosaponin biosynthesis differentially expressed gene |
| url | https://www.mdpi.com/2075-1729/13/2/563 |
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